Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers
Postharvest diseases of potato lead to significant food and economic losses worldwide. The exogenous application of eco-friendly methods plays an important role in the control of postharvest decay. In this work the effects of endophytic bacteria <i>B. subtilis</i> (10-4, 26D) were studie...
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MDPI AG
2020-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/1/76 |
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| author | Oksana Lastochkina Andrey Baymiev Aysylu Shayahmetova Darya Garshina Igor Koryakov Irina Shpirnaya Liudmila Pusenkova Il’dar Mardanshin Cemal Kasnak Recep Palamutoglu |
| author_facet | Oksana Lastochkina Andrey Baymiev Aysylu Shayahmetova Darya Garshina Igor Koryakov Irina Shpirnaya Liudmila Pusenkova Il’dar Mardanshin Cemal Kasnak Recep Palamutoglu |
| author_sort | Oksana Lastochkina |
| collection | DOAJ |
| description | Postharvest diseases of potato lead to significant food and economic losses worldwide. The exogenous application of eco-friendly methods plays an important role in the control of postharvest decay. In this work the effects of endophytic bacteria <i>B. subtilis</i> (10-4, 26D) were studied in the context of two application parameters: concentration, with a range between 10<sup>3</sup>−10<sup>8</sup> CFU/mL tested, and synergistic effects of the signal molecule salicylic acid (SA) (0.05 mM) on potato tubers’ resistance to <i>Phytophthora infestans</i> and <i>Fusarium oxysporum</i> during storage. The experiments were carried out on hydroponically grown potato (<i>Solanum tuberosum</i> L.) mini-tubers. This study demonstrates the suppressive effect of <i>B. subtilis</i> (10-4, 26D) on diseases of potato during storage and reveals that this effect happens in a dose-dependent manner, both individually and in combination with SA. The most effective concentrations of <i>B. subtilis</i> for suppression of both <i>Ph.</i> <i>infestans</i> and <i>F. oxysporum</i> are 10<sup>8</sup> CFU/mL (10-4 and 26D), 10<sup>7</sup> CFU/mL (10-4 + SA) and 10<sup>6</sup> CFU/mL (26D + SA). The ability of <i>B. subtilis</i> (10-4, 26D) to effectively penetrate and colonize the internal tubers’ tissues when applied immediately prior to storage, and the ability of SA to accelerate these processes, have been proven. <i>B. subtilis</i> (10-4, 26D), individually and in compositions with SA, increased ascorbic acid content and decreased pathogen-induced proline accumulation and lipid peroxidation in tubers. This indicates a protective effect conferred to cells against reactive oxygen and an extension of aging processes, manifested by a prolonged shelf life and extended preservation of fresh appearance. |
| first_indexed | 2024-04-11T16:01:29Z |
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| last_indexed | 2024-04-11T16:01:29Z |
| publishDate | 2020-01-01 |
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| spelling | doaj.art-7488fb7c05264ed1bcf9fe3c85627e7c2022-12-22T04:15:01ZengMDPI AGPlants2223-77472020-01-01917610.3390/plants9010076plants9010076Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato TubersOksana Lastochkina0Andrey Baymiev1Aysylu Shayahmetova2Darya Garshina3Igor Koryakov4Irina Shpirnaya5Liudmila Pusenkova6Il’dar Mardanshin7Cemal Kasnak8Recep Palamutoglu9Ufa Federal Research Centre of the Russian Academy of Sciences, Bashkir Research Institute of Agriculture, Ufa 450059, RussiaInstitute of Biochemistry and Genetics, Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaUfa Federal Research Centre of the Russian Academy of Sciences, Bashkir Research Institute of Agriculture, Ufa 450059, RussiaUfa Federal Research Centre of the Russian Academy of Sciences, Bashkir Research Institute of Agriculture, Ufa 450059, RussiaInstitute of Biochemistry and Genetics, Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa 450054, RussiaDepartment of Biology, Bashkir State University, Ufa 450074, RussiaUfa Federal Research Centre of the Russian Academy of Sciences, Bashkir Research Institute of Agriculture, Ufa 450059, RussiaUfa Federal Research Centre of the Russian Academy of Sciences, Bashkir Research Institute of Agriculture, Ufa 450059, RussiaDepartment of Nutrition and Dietetics, Health Science Faculty, Afyonkarahisar Health Science University, 2078 Afyon, TurkeyDepartment of Nutrition and Dietetics, Health Science Faculty, Afyonkarahisar Health Science University, 2078 Afyon, TurkeyPostharvest diseases of potato lead to significant food and economic losses worldwide. The exogenous application of eco-friendly methods plays an important role in the control of postharvest decay. In this work the effects of endophytic bacteria <i>B. subtilis</i> (10-4, 26D) were studied in the context of two application parameters: concentration, with a range between 10<sup>3</sup>−10<sup>8</sup> CFU/mL tested, and synergistic effects of the signal molecule salicylic acid (SA) (0.05 mM) on potato tubers’ resistance to <i>Phytophthora infestans</i> and <i>Fusarium oxysporum</i> during storage. The experiments were carried out on hydroponically grown potato (<i>Solanum tuberosum</i> L.) mini-tubers. This study demonstrates the suppressive effect of <i>B. subtilis</i> (10-4, 26D) on diseases of potato during storage and reveals that this effect happens in a dose-dependent manner, both individually and in combination with SA. The most effective concentrations of <i>B. subtilis</i> for suppression of both <i>Ph.</i> <i>infestans</i> and <i>F. oxysporum</i> are 10<sup>8</sup> CFU/mL (10-4 and 26D), 10<sup>7</sup> CFU/mL (10-4 + SA) and 10<sup>6</sup> CFU/mL (26D + SA). The ability of <i>B. subtilis</i> (10-4, 26D) to effectively penetrate and colonize the internal tubers’ tissues when applied immediately prior to storage, and the ability of SA to accelerate these processes, have been proven. <i>B. subtilis</i> (10-4, 26D), individually and in compositions with SA, increased ascorbic acid content and decreased pathogen-induced proline accumulation and lipid peroxidation in tubers. This indicates a protective effect conferred to cells against reactive oxygen and an extension of aging processes, manifested by a prolonged shelf life and extended preservation of fresh appearance.https://www.mdpi.com/2223-7747/9/1/76endophytic <i>bacillus subtilis</i>salicylic acidpostharvest diseases<i>phytophthora infestans</i><i>fusarium oxysporum</i>resistancepotatoes |
| spellingShingle | Oksana Lastochkina Andrey Baymiev Aysylu Shayahmetova Darya Garshina Igor Koryakov Irina Shpirnaya Liudmila Pusenkova Il’dar Mardanshin Cemal Kasnak Recep Palamutoglu Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers Plants endophytic <i>bacillus subtilis</i> salicylic acid postharvest diseases <i>phytophthora infestans</i> <i>fusarium oxysporum</i> resistance potatoes |
| title | Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers |
| title_full | Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers |
| title_fullStr | Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers |
| title_full_unstemmed | Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers |
| title_short | Effects of Endophytic <i>Bacillus Subtilis</i> and Salicylic Acid on Postharvest Diseases (<i>Phytophthora infestans, Fusarium oxysporum</i>) Development in Stored Potato Tubers |
| title_sort | effects of endophytic i bacillus subtilis i and salicylic acid on postharvest diseases i phytophthora infestans fusarium oxysporum i development in stored potato tubers |
| topic | endophytic <i>bacillus subtilis</i> salicylic acid postharvest diseases <i>phytophthora infestans</i> <i>fusarium oxysporum</i> resistance potatoes |
| url | https://www.mdpi.com/2223-7747/9/1/76 |
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