Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’
Leaf pubescence is an important trait closely associated with plant adaptability to specialized habitats. Baimaomai (BMM) is a wheat (<i>Triticum aestivum</i> L.) landrace originating from the high-altitude, drought-prone environment of Sichuan Province, China with long, dense leaf pubes...
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MDPI AG
2021-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/11/2237 |
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| author | Zehou Liu Qin Wang Hongshen Wan Fan Yang Shizhao Li Manyu Yang Jiangtao Luo Jianmin Zheng Qingyan Deng Hai Long Guangbing Deng Ning Yang Jun Li Wuyun Yang |
| author_facet | Zehou Liu Qin Wang Hongshen Wan Fan Yang Shizhao Li Manyu Yang Jiangtao Luo Jianmin Zheng Qingyan Deng Hai Long Guangbing Deng Ning Yang Jun Li Wuyun Yang |
| author_sort | Zehou Liu |
| collection | DOAJ |
| description | Leaf pubescence is an important trait closely associated with plant adaptability to specialized habitats. Baimaomai (BMM) is a wheat (<i>Triticum aestivum</i> L.) landrace originating from the high-altitude, drought-prone environment of Sichuan Province, China with long, dense leaf pubescence. A population of 234 recombinant inbred lines (F<sub>10</sub>) developed from the cross between Chuanmai104 (CM104), which lacks leaf pubescence, and BMM with pubescent leaves, was used to conduct a phenotypic evaluation of leaf pubescence. Three quantitative trait loci (QTLs) were detected on chromosome arms 7BS, 3DL and 3AL using a high-density wheat 50K single-nucleotide polymorphism array in four environments. The QTLs were designated <i>QLp.saas-7BS</i>, <i>QLp.saas-3DL</i> and <i>QLp.saas-3AL</i>. <i>QLp.saas-3AL</i>, derived from BMM, and <i>QLp.saas-3DL</i>, derived from CM104, were new minor-effect loci. <i>QLp.saas-7BS</i>, derived from BMM, was a novel major-effect locus detected in all environments and was localized in a 0.48 Mb interval on chromosome arm 7BS based on the wheat ‘Chinese Spring’ reference genome. <i>QLp.saas-7BS</i> explained up to 40.77% of the total phenotypic variance. KASP markers tightly linked to <i>QLp.saas-7BS</i> were developed and verified. The present results provide valuable information for further fine mapping, cloning, and marker-assisted selection with <i>QLp.saas-7BS</i> in wheat. |
| first_indexed | 2024-03-10T05:47:07Z |
| format | Article |
| id | doaj.art-748a4fd33a634f1bbfec05ec00e91ff7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:47:07Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-748a4fd33a634f1bbfec05ec00e91ff72023-11-22T22:02:36ZengMDPI AGAgronomy2073-43952021-11-011111223710.3390/agronomy11112237Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’Zehou Liu0Qin Wang1Hongshen Wan2Fan Yang3Shizhao Li4Manyu Yang5Jiangtao Luo6Jianmin Zheng7Qingyan Deng8Hai Long9Guangbing Deng10Ning Yang11Jun Li12Wuyun Yang13Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaKey Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture and Rural Affairs), Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaLeaf pubescence is an important trait closely associated with plant adaptability to specialized habitats. Baimaomai (BMM) is a wheat (<i>Triticum aestivum</i> L.) landrace originating from the high-altitude, drought-prone environment of Sichuan Province, China with long, dense leaf pubescence. A population of 234 recombinant inbred lines (F<sub>10</sub>) developed from the cross between Chuanmai104 (CM104), which lacks leaf pubescence, and BMM with pubescent leaves, was used to conduct a phenotypic evaluation of leaf pubescence. Three quantitative trait loci (QTLs) were detected on chromosome arms 7BS, 3DL and 3AL using a high-density wheat 50K single-nucleotide polymorphism array in four environments. The QTLs were designated <i>QLp.saas-7BS</i>, <i>QLp.saas-3DL</i> and <i>QLp.saas-3AL</i>. <i>QLp.saas-3AL</i>, derived from BMM, and <i>QLp.saas-3DL</i>, derived from CM104, were new minor-effect loci. <i>QLp.saas-7BS</i>, derived from BMM, was a novel major-effect locus detected in all environments and was localized in a 0.48 Mb interval on chromosome arm 7BS based on the wheat ‘Chinese Spring’ reference genome. <i>QLp.saas-7BS</i> explained up to 40.77% of the total phenotypic variance. KASP markers tightly linked to <i>QLp.saas-7BS</i> were developed and verified. The present results provide valuable information for further fine mapping, cloning, and marker-assisted selection with <i>QLp.saas-7BS</i> in wheat.https://www.mdpi.com/2073-4395/11/11/2237wheatlandraceBaimaomaileaf pubescenceQTL |
| spellingShingle | Zehou Liu Qin Wang Hongshen Wan Fan Yang Shizhao Li Manyu Yang Jiangtao Luo Jianmin Zheng Qingyan Deng Hai Long Guangbing Deng Ning Yang Jun Li Wuyun Yang Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ Agronomy wheat landrace Baimaomai leaf pubescence QTL |
| title | Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ |
| title_full | Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ |
| title_fullStr | Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ |
| title_full_unstemmed | Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ |
| title_short | Identification and Validation of a Novel Major QTL Controlling Leaf Pubescence in the Chinese Wheat Landrace ‘Baimaomai’ |
| title_sort | identification and validation of a novel major qtl controlling leaf pubescence in the chinese wheat landrace baimaomai |
| topic | wheat landrace Baimaomai leaf pubescence QTL |
| url | https://www.mdpi.com/2073-4395/11/11/2237 |
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