Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain
Zika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of <...
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| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/2/130 |
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| author | Alioune Gaye Cheikh Fall Oumar Faye Myrielle Dupont-Rouzeyrol El Hadji Ndiaye Diawo Diallo Paolo Marinho de Andrade Zanotto Ibrahima Dia Scott C. Weaver Mawlouth Diallo |
| author_facet | Alioune Gaye Cheikh Fall Oumar Faye Myrielle Dupont-Rouzeyrol El Hadji Ndiaye Diawo Diallo Paolo Marinho de Andrade Zanotto Ibrahima Dia Scott C. Weaver Mawlouth Diallo |
| author_sort | Alioune Gaye |
| collection | DOAJ |
| description | Zika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of <i>A. aegypti</i> and <i>C. quinquefasciatus</i> to ZIKV strains from Senegal, Brazil, and New Caledonia. Vertical transmission was also investigated. Whole bodies, legs/wings and saliva samples were tested for ZIKV by real-time PCR to estimate infection, dissemination and transmission rates as well as the infection rate in the progeny of infected female <i>A. aegypti</i>. For <i>A. aegypti</i>, the Senegalese strain showed at 15 days post-exposure (dpe) a significantly higher infection rate (52.43%) than the Brazilian (10%) and New Caledonian (0%) strains. The Brazilian and Senegalese strains were disseminated but not detected in saliva. No <i>A. aegypti</i> offspring from females infected with Senegalese and Brazilian ZIKV strains tested positive. No infection was recorded for <i>C. quinquefasciatus</i>. We observed the incompetence of Senegalese <i>A. aegypti</i> to transmit ZIKV and the <i>C. quinquefasciatus</i> were completely refractory. The effect of freezing ZIKV had no significant impact on the vector competence of <i>Aedes aegypti</i> from Senegal, and vertical transmission was not reported in this study. |
| first_indexed | 2024-03-11T08:02:54Z |
| format | Article |
| id | doaj.art-748ec3c8e5fd446eb4e58bec4b5ea8f8 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-11T08:02:54Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-748ec3c8e5fd446eb4e58bec4b5ea8f82023-11-16T23:40:20ZengMDPI AGTropical Medicine and Infectious Disease2414-63662023-02-018213010.3390/tropicalmed8020130Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native StrainAlioune Gaye0Cheikh Fall1Oumar Faye2Myrielle Dupont-Rouzeyrol3El Hadji Ndiaye4Diawo Diallo5Paolo Marinho de Andrade Zanotto6Ibrahima Dia7Scott C. Weaver8Mawlouth Diallo9Pole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalPole de Microbiologie, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalPole de Virologie, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalURE Dengue et Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur, BP 61, CEDEX, 98845 Noumea, New CaledoniaPole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalPole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalMicrobiology Department, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo 05508020, SP, BrazilPole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalWorld Reference Center for Emerging Viruses and Arboviruses, Institute for Human Infections and Immunity, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USAPole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, SenegalZika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of <i>A. aegypti</i> and <i>C. quinquefasciatus</i> to ZIKV strains from Senegal, Brazil, and New Caledonia. Vertical transmission was also investigated. Whole bodies, legs/wings and saliva samples were tested for ZIKV by real-time PCR to estimate infection, dissemination and transmission rates as well as the infection rate in the progeny of infected female <i>A. aegypti</i>. For <i>A. aegypti</i>, the Senegalese strain showed at 15 days post-exposure (dpe) a significantly higher infection rate (52.43%) than the Brazilian (10%) and New Caledonian (0%) strains. The Brazilian and Senegalese strains were disseminated but not detected in saliva. No <i>A. aegypti</i> offspring from females infected with Senegalese and Brazilian ZIKV strains tested positive. No infection was recorded for <i>C. quinquefasciatus</i>. We observed the incompetence of Senegalese <i>A. aegypti</i> to transmit ZIKV and the <i>C. quinquefasciatus</i> were completely refractory. The effect of freezing ZIKV had no significant impact on the vector competence of <i>Aedes aegypti</i> from Senegal, and vertical transmission was not reported in this study.https://www.mdpi.com/2414-6366/8/2/130Zika virus<i>A. aegypti</i><i>C. quinquefasciatus</i>BrazilNew CaledoniaSenegal |
| spellingShingle | Alioune Gaye Cheikh Fall Oumar Faye Myrielle Dupont-Rouzeyrol El Hadji Ndiaye Diawo Diallo Paolo Marinho de Andrade Zanotto Ibrahima Dia Scott C. Weaver Mawlouth Diallo Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain Tropical Medicine and Infectious Disease Zika virus <i>A. aegypti</i> <i>C. quinquefasciatus</i> Brazil New Caledonia Senegal |
| title | Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain |
| title_full | Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain |
| title_fullStr | Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain |
| title_full_unstemmed | Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain |
| title_short | Assessment of the Risk of Exotic Zika Virus Strain Transmission by <i>Aedes aegypti</i> and <i>Culex quinquefasciatus</i> from Senegal Compared to a Native Strain |
| title_sort | assessment of the risk of exotic zika virus strain transmission by i aedes aegypti i and i culex quinquefasciatus i from senegal compared to a native strain |
| topic | Zika virus <i>A. aegypti</i> <i>C. quinquefasciatus</i> Brazil New Caledonia Senegal |
| url | https://www.mdpi.com/2414-6366/8/2/130 |
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