Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates

Introduction: Escherichia coli is one of the most common bacteria causing urinary tract infection. Accurate identification of urinary isolates is highly desirable and frequently challenging. Our study aims at identifying the “atypical” phenotype of E. coli by conventional, automated, and molecular m...

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Main Authors: Trupti Bajpai, Maneesha Pandey, Meena Varma, Ganesh Bhatambare
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:CHRISMED Journal of Health and Research
Subjects:
Online Access:http://www.cjhr.org/article.asp?issn=2348-3334;year=2016;volume=3;issue=4;spage=288;epage=290;aulast=Bajpai
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author Trupti Bajpai
Maneesha Pandey
Meena Varma
Ganesh Bhatambare
author_facet Trupti Bajpai
Maneesha Pandey
Meena Varma
Ganesh Bhatambare
author_sort Trupti Bajpai
collection DOAJ
description Introduction: Escherichia coli is one of the most common bacteria causing urinary tract infection. Accurate identification of urinary isolates is highly desirable and frequently challenging. Our study aims at identifying the “atypical” phenotype of E. coli by conventional, automated, and molecular methods and studying its prevalence among all the urinary isolates of E. coli. Materials and Methods: The present prospective study was conducted in the Department of Microbiology of a teaching tertiary care hospital of Central India for 6 months during the year 2014–2015. A total of 592 urine samples were processed. Identification of different urinary isolates was done by conventional and automated methods and by molecular method in special cases. Results: Two hundred and sixty-one uropathogens were isolated during the study. Among these, 110 (42.1%) isolates were E. coli, of which 4 (3.6%) isolates were confirmed as “atypical” E. coli by automated and molecular method. Conclusions: Our study highlights the challenges in the identification of atypical urinary isolates of E. coli. Accurate identification is essential for implicating proper antibiotic treatment.
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spelling doaj.art-74903357edc343c9aca6061aa4974e5a2022-12-21T19:14:35ZengWolters Kluwer Medknow PublicationsCHRISMED Journal of Health and Research2348-33342348-506X2016-01-013428829010.4103/2348-3334.190581Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolatesTrupti BajpaiManeesha PandeyMeena VarmaGanesh BhatambareIntroduction: Escherichia coli is one of the most common bacteria causing urinary tract infection. Accurate identification of urinary isolates is highly desirable and frequently challenging. Our study aims at identifying the “atypical” phenotype of E. coli by conventional, automated, and molecular methods and studying its prevalence among all the urinary isolates of E. coli. Materials and Methods: The present prospective study was conducted in the Department of Microbiology of a teaching tertiary care hospital of Central India for 6 months during the year 2014–2015. A total of 592 urine samples were processed. Identification of different urinary isolates was done by conventional and automated methods and by molecular method in special cases. Results: Two hundred and sixty-one uropathogens were isolated during the study. Among these, 110 (42.1%) isolates were E. coli, of which 4 (3.6%) isolates were confirmed as “atypical” E. coli by automated and molecular method. Conclusions: Our study highlights the challenges in the identification of atypical urinary isolates of E. coli. Accurate identification is essential for implicating proper antibiotic treatment.http://www.cjhr.org/article.asp?issn=2348-3334;year=2016;volume=3;issue=4;spage=288;epage=290;aulast=Bajpai16S ribosomal RNA sequencingatypical Escherichia coliVITEK-2 Compact
spellingShingle Trupti Bajpai
Maneesha Pandey
Meena Varma
Ganesh Bhatambare
Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
CHRISMED Journal of Health and Research
16S ribosomal RNA sequencing
atypical Escherichia coli
VITEK-2 Compact
title Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
title_full Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
title_fullStr Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
title_full_unstemmed Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
title_short Importance of identification of lactose nonfermenting Escherichia coli and their prevalence in urinary isolates
title_sort importance of identification of lactose nonfermenting escherichia coli and their prevalence in urinary isolates
topic 16S ribosomal RNA sequencing
atypical Escherichia coli
VITEK-2 Compact
url http://www.cjhr.org/article.asp?issn=2348-3334;year=2016;volume=3;issue=4;spage=288;epage=290;aulast=Bajpai
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AT meenavarma importanceofidentificationoflactosenonfermentingescherichiacoliandtheirprevalenceinurinaryisolates
AT ganeshbhatambare importanceofidentificationoflactosenonfermentingescherichiacoliandtheirprevalenceinurinaryisolates