Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria
Lactic acid bacteria (LAB) bacteriocins can be considered as a bio-preservatives and an alternative to antibiotics, but the high manufacturing costs limit their commercial application. The screening of LAB strains for bacteriocinogenic activity was carried out and the effect of rye bran enzymatic hy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/12/752 |
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| author | Julia M. Epishkina Maria V. Romanova Marina A. Chalenko Natalya Yu. Khromova Boris A. Karetkin Andrey V. Beloded Maria A. Kornienko Yulia M. Averina Irina V. Shakir Victor I. Panfilov |
| author_facet | Julia M. Epishkina Maria V. Romanova Marina A. Chalenko Natalya Yu. Khromova Boris A. Karetkin Andrey V. Beloded Maria A. Kornienko Yulia M. Averina Irina V. Shakir Victor I. Panfilov |
| author_sort | Julia M. Epishkina |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) bacteriocins can be considered as a bio-preservatives and an alternative to antibiotics, but the high manufacturing costs limit their commercial application. The screening of LAB strains for bacteriocinogenic activity was carried out and the effect of rye bran enzymatic hydrolysate (RBEH) on gene expression and bacteriocin production was evaluated. qPCR and RT-PCR was applied for bacteriocin gene detection and their expression quantification. The agar diffusion technique with the test strains of <i>Bacillus</i> spp., <i>Staphylococcus</i> spp. and <i>Salmonella enterica</i> was performed for antimicrobial activity assessment of LAB cultivated in MRS broth and RBEH (processed with proteases and cellulases). The genes of different bacteriocins were revealed for thirteen out of eighteen LAB strains, while the antimicrobial activity was detected only for four of them. The strains of <i>Lactobacillus paracasei</i> VKPM B-11657 and <i>L. salivarius</i> VKPM B-2214 with unnamed class IIb bacteriocin gene demonstrated the widest spectrum of activity. The growth patterns and bacteriocin gene expression differed between both strains and media. The activity of cell-free supernatants after cultivation in RBEH was slightly lower. However, the test strain of <i>S. epidermidis</i> was inhibited by <i>L. paracasei</i> cultivated in RBEH but not in MRS. Thus, rye bran can be applied as a sole source of nutrients for LAB fermentation and bacteriocin production. |
| first_indexed | 2024-03-09T16:45:57Z |
| format | Article |
| id | doaj.art-74929f1a3526405191feb0aaed365a9e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T16:45:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-74929f1a3526405191feb0aaed365a9e2023-11-24T14:46:15ZengMDPI AGFermentation2311-56372022-12-0181275210.3390/fermentation8120752Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid BacteriaJulia M. Epishkina0Maria V. Romanova1Marina A. Chalenko2Natalya Yu. Khromova3Boris A. Karetkin4Andrey V. Beloded5Maria A. Kornienko6Yulia M. Averina7Irina V. Shakir8Victor I. Panfilov9Department of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaDepartment of Logistics and Economic Information, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaDepartment of Biotechnology, Faculty of Biotechnology and Industrial Ecology, Mendeleev University of Chemical Technology, 125047 Moscow, RussiaLactic acid bacteria (LAB) bacteriocins can be considered as a bio-preservatives and an alternative to antibiotics, but the high manufacturing costs limit their commercial application. The screening of LAB strains for bacteriocinogenic activity was carried out and the effect of rye bran enzymatic hydrolysate (RBEH) on gene expression and bacteriocin production was evaluated. qPCR and RT-PCR was applied for bacteriocin gene detection and their expression quantification. The agar diffusion technique with the test strains of <i>Bacillus</i> spp., <i>Staphylococcus</i> spp. and <i>Salmonella enterica</i> was performed for antimicrobial activity assessment of LAB cultivated in MRS broth and RBEH (processed with proteases and cellulases). The genes of different bacteriocins were revealed for thirteen out of eighteen LAB strains, while the antimicrobial activity was detected only for four of them. The strains of <i>Lactobacillus paracasei</i> VKPM B-11657 and <i>L. salivarius</i> VKPM B-2214 with unnamed class IIb bacteriocin gene demonstrated the widest spectrum of activity. The growth patterns and bacteriocin gene expression differed between both strains and media. The activity of cell-free supernatants after cultivation in RBEH was slightly lower. However, the test strain of <i>S. epidermidis</i> was inhibited by <i>L. paracasei</i> cultivated in RBEH but not in MRS. Thus, rye bran can be applied as a sole source of nutrients for LAB fermentation and bacteriocin production.https://www.mdpi.com/2311-5637/8/12/752lactic acid bacteriabacteriocinsantimicrobial activityquantitative polymerase chain reactiongene expressiongreen raw materials |
| spellingShingle | Julia M. Epishkina Maria V. Romanova Marina A. Chalenko Natalya Yu. Khromova Boris A. Karetkin Andrey V. Beloded Maria A. Kornienko Yulia M. Averina Irina V. Shakir Victor I. Panfilov Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria Fermentation lactic acid bacteria bacteriocins antimicrobial activity quantitative polymerase chain reaction gene expression green raw materials |
| title | Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria |
| title_full | Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria |
| title_fullStr | Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria |
| title_full_unstemmed | Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria |
| title_short | Evaluation of Rye Bran Enzymatic Hydrolysate Effect on Gene Expression and Bacteriocinogenic Activity of Lactic Acid Bacteria |
| title_sort | evaluation of rye bran enzymatic hydrolysate effect on gene expression and bacteriocinogenic activity of lactic acid bacteria |
| topic | lactic acid bacteria bacteriocins antimicrobial activity quantitative polymerase chain reaction gene expression green raw materials |
| url | https://www.mdpi.com/2311-5637/8/12/752 |
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