Dietary Safety Assessment of Flk1-Transgenic Fish
Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00008/full |
| _version_ | 1818334406455066624 |
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| author | Yalan Wei Ling Huang Jinghui Cao Chenghui Wang Jizhou Yan |
| author_facet | Yalan Wei Ling Huang Jinghui Cao Chenghui Wang Jizhou Yan |
| author_sort | Yalan Wei |
| collection | DOAJ |
| description | Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding Flk1-transgenic larvae model to examine the predatory fish's histology in multiple tissues, and the global gene expression profile in the liver. 180 days of feeding trial show that there are no significantly morphological changes in intestine, liver, kidney, and sex gonads between fish fed with Flk1 transgenic fish diet (TFD) and fish fed with regular food meal (RFM). However, a characteristic skin spot and autofluorescence increase in the theca of follicle are observed in F1 generation of TFD fish. Liver RNA-sequencing analyses demonstrate that 53 out of 56712 genes or isoforms are differentially transcribed, and mostly involved in proteolysis in extracellular region. According to GO enrichment terms these deregulated genes function in catalytic activity, steroid storing, lipid metabolic process and N-Glycan biosynthesis. These results suggest that a long term of Flk1-transgenic fish diet could alter certain metabolic pathways and possibly cause related tissue deformation. Compared to the previous reports, our feasible transgenic dietary assess system could evaluate subchronic and potential health impact of transgenic fish diet by combining multi-tissue histology and liver transcriptome analyses. |
| first_indexed | 2024-12-13T14:07:02Z |
| format | Article |
| id | doaj.art-74940a7250ed46c9af46437ccb26a2fe |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-13T14:07:02Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-74940a7250ed46c9af46437ccb26a2fe2022-12-21T23:42:35ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-01-01910.3389/fphys.2018.00008332676Dietary Safety Assessment of Flk1-Transgenic FishYalan WeiLing HuangJinghui CaoChenghui WangJizhou YanGenetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding Flk1-transgenic larvae model to examine the predatory fish's histology in multiple tissues, and the global gene expression profile in the liver. 180 days of feeding trial show that there are no significantly morphological changes in intestine, liver, kidney, and sex gonads between fish fed with Flk1 transgenic fish diet (TFD) and fish fed with regular food meal (RFM). However, a characteristic skin spot and autofluorescence increase in the theca of follicle are observed in F1 generation of TFD fish. Liver RNA-sequencing analyses demonstrate that 53 out of 56712 genes or isoforms are differentially transcribed, and mostly involved in proteolysis in extracellular region. According to GO enrichment terms these deregulated genes function in catalytic activity, steroid storing, lipid metabolic process and N-Glycan biosynthesis. These results suggest that a long term of Flk1-transgenic fish diet could alter certain metabolic pathways and possibly cause related tissue deformation. Compared to the previous reports, our feasible transgenic dietary assess system could evaluate subchronic and potential health impact of transgenic fish diet by combining multi-tissue histology and liver transcriptome analyses.http://journal.frontiersin.org/article/10.3389/fphys.2018.00008/fullgenetic modificationtransgenic fishdietary safetyRNA-sequencingzebrafish |
| spellingShingle | Yalan Wei Ling Huang Jinghui Cao Chenghui Wang Jizhou Yan Dietary Safety Assessment of Flk1-Transgenic Fish Frontiers in Physiology genetic modification transgenic fish dietary safety RNA-sequencing zebrafish |
| title | Dietary Safety Assessment of Flk1-Transgenic Fish |
| title_full | Dietary Safety Assessment of Flk1-Transgenic Fish |
| title_fullStr | Dietary Safety Assessment of Flk1-Transgenic Fish |
| title_full_unstemmed | Dietary Safety Assessment of Flk1-Transgenic Fish |
| title_short | Dietary Safety Assessment of Flk1-Transgenic Fish |
| title_sort | dietary safety assessment of flk1 transgenic fish |
| topic | genetic modification transgenic fish dietary safety RNA-sequencing zebrafish |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00008/full |
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