Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats
Diabetes mellitus is a chronic metabolic disorder defined as hyperglycemia and pancreatic β-cell deterioration, leading to other complications such as cardiomyopathy. The current study assessed the therapeutic effects of phenolic acids extracted from <i>Jasminum sambac</i> phenols of lea...
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MDPI AG
2023-07-01
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| author | Urooj Umar Sibtain Ahmed Asra Iftikhar Maryam Iftikhar Wafa Majeed Atika Liaqat Sana Shahzad Mateen Abbas Tahir Mehmood Farooq Anwar |
| author_facet | Urooj Umar Sibtain Ahmed Asra Iftikhar Maryam Iftikhar Wafa Majeed Atika Liaqat Sana Shahzad Mateen Abbas Tahir Mehmood Farooq Anwar |
| author_sort | Urooj Umar |
| collection | DOAJ |
| description | Diabetes mellitus is a chronic metabolic disorder defined as hyperglycemia and pancreatic β-cell deterioration, leading to other complications such as cardiomyopathy. The current study assessed the therapeutic effects of phenolic acids extracted from <i>Jasminum sambac</i> phenols of leaves (JSP) against diabetes-induced cardiomyopathy in rats. The rats were divided into four groups, with each group consisting of 20 rats. The rats were given intraperitoneal injections of alloxan monohydrate (150 mg/kg) to induce diabetes. The diabetes-induced groups (III and IV) received treatment for six weeks that included 250 and 500 mg/kg of JSP extract, respectively. In the treated rats, the results demonstrated that JSP extract restored fasting glucose, serum glucose, and hyperlipidemia. Alloxan induced cardiomyopathy, promoted oxidative stress, and altered cardiac function biomarkers, including cardiac troponin I, proBNP, CK-MB, LDH, and IMA. The JSP extract-treated rats showed improved cardiac function indicators, apoptosis, and oxidative stress. In diabetic rats, the mRNA expression of caspase-3, BAX, and Bcl-2 was significantly higher, while Bcl-2, Nrf-2, and HO-,1 was significantly lower. In the treated groups, the expression levels of the BAX, Nrf-2, HO-1, Caspase-3, and Bcl-2 genes were dramatically returned to normal level. According to our findings, the JSP extract prevented cardiomyopathy and heart failure in the hyperglycemic rats by improving cardiac biomarkers and lowering the levels of hyperlipidemia, oxidative stress, apoptosis, hyperglycemia, and hyperlipidemia. |
| first_indexed | 2024-03-11T00:46:58Z |
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| id | doaj.art-74963a935b514aebb7d721af80d3f6c9 |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:46:58Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-74963a935b514aebb7d721af80d3f6c92023-11-18T20:42:20ZengMDPI AGMolecules1420-30492023-07-012814545310.3390/molecules28145453Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic RatsUrooj Umar0Sibtain Ahmed1Asra Iftikhar2Maryam Iftikhar3Wafa Majeed4Atika Liaqat5Sana Shahzad6Mateen Abbas7Tahir Mehmood8Farooq Anwar9Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of Faisalabad, Faisalabad 38000, PakistanDepartment of Biochemistry, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Pharmacy, Faculty of Pharmaceutical Sciences, University of Faisalabad, Faisalabad 38000, PakistanInstitute of Home & Food Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmacy, University of Agriculture, Faisalabad 38000, PakistanDepartment of Pharmacy, Faculty of Pharmaceutical Sciences, University of Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmacy, Faculty of Pharmaceutical Sciences, University of Faisalabad, Faisalabad 38000, PakistanQuality Operations Laboratory, Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, PakistanCentre for Applied Molecular Biology (CAMB), University of the Punjab, Lahore 53700, PakistanDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, MalaysiaDiabetes mellitus is a chronic metabolic disorder defined as hyperglycemia and pancreatic β-cell deterioration, leading to other complications such as cardiomyopathy. The current study assessed the therapeutic effects of phenolic acids extracted from <i>Jasminum sambac</i> phenols of leaves (JSP) against diabetes-induced cardiomyopathy in rats. The rats were divided into four groups, with each group consisting of 20 rats. The rats were given intraperitoneal injections of alloxan monohydrate (150 mg/kg) to induce diabetes. The diabetes-induced groups (III and IV) received treatment for six weeks that included 250 and 500 mg/kg of JSP extract, respectively. In the treated rats, the results demonstrated that JSP extract restored fasting glucose, serum glucose, and hyperlipidemia. Alloxan induced cardiomyopathy, promoted oxidative stress, and altered cardiac function biomarkers, including cardiac troponin I, proBNP, CK-MB, LDH, and IMA. The JSP extract-treated rats showed improved cardiac function indicators, apoptosis, and oxidative stress. In diabetic rats, the mRNA expression of caspase-3, BAX, and Bcl-2 was significantly higher, while Bcl-2, Nrf-2, and HO-,1 was significantly lower. In the treated groups, the expression levels of the BAX, Nrf-2, HO-1, Caspase-3, and Bcl-2 genes were dramatically returned to normal level. According to our findings, the JSP extract prevented cardiomyopathy and heart failure in the hyperglycemic rats by improving cardiac biomarkers and lowering the levels of hyperlipidemia, oxidative stress, apoptosis, hyperglycemia, and hyperlipidemia.https://www.mdpi.com/1420-3049/28/14/5453<i>Jasminum sambac</i>alloxan monohydratecardiomyopathyoxidative stresscardiac function markers |
| spellingShingle | Urooj Umar Sibtain Ahmed Asra Iftikhar Maryam Iftikhar Wafa Majeed Atika Liaqat Sana Shahzad Mateen Abbas Tahir Mehmood Farooq Anwar Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats Molecules <i>Jasminum sambac</i> alloxan monohydrate cardiomyopathy oxidative stress cardiac function markers |
| title | Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats |
| title_full | Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats |
| title_fullStr | Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats |
| title_full_unstemmed | Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats |
| title_short | Phenolics Extracted from <i>Jasminum sambac</i> Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats |
| title_sort | phenolics extracted from i jasminum sambac i mitigates diabetic cardiomyopathy by modulating oxidative stress apoptotic mediators and the nfr 2 ho 1 pathway in alloxan induced diabetic rats |
| topic | <i>Jasminum sambac</i> alloxan monohydrate cardiomyopathy oxidative stress cardiac function markers |
| url | https://www.mdpi.com/1420-3049/28/14/5453 |
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