Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease
Enteric glial cells (EGC) are known to regulate gastrointestinal functions; however, their role in Crohn’s disease (CD) is elusive. Microscopic erosions over the ileal Peyer’s patches are early signs of CD. The aim of this work was to assess the localization of EGC in the follicle and interfollicula...
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MDPI AG
2022-01-01
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| author | Olga Biskou Felipe Meira de-Faria Susanna M. Walter Martin E. Winberg Staffan Haapaniemi Pär Myrelid Johan D. Söderholm Åsa V. Keita |
| author_facet | Olga Biskou Felipe Meira de-Faria Susanna M. Walter Martin E. Winberg Staffan Haapaniemi Pär Myrelid Johan D. Söderholm Åsa V. Keita |
| author_sort | Olga Biskou |
| collection | DOAJ |
| description | Enteric glial cells (EGC) are known to regulate gastrointestinal functions; however, their role in Crohn’s disease (CD) is elusive. Microscopic erosions over the ileal Peyer’s patches are early signs of CD. The aim of this work was to assess the localization of EGC in the follicle and interfollicular region of the Peyer’s patches and in the lamina propria and study the effects of EGC mediators on barrier function in CD patients and non-inflammatory bowel disease (non-IBD) controls. EGC markers, glial fibrillary acidic protein (GFAP), and S100 calcium-binding protein β (S100β) were quantified by immunofluorescence and Western blotting. Both markers showed significantly more EGC in the Peyer’s patches and lamina propria of CD patients compared to the non-IBD controls. In CD patients there were significantly more EGC in Peyer’s patches compared to lamina propria, while the opposite pattern was seen in controls. Barrier function studies using Ussing chambers showed increased paracellular permeability by EGC mediators in CD patients, whereas permeability decreased by the mediators in controls. We show the accumulation of EGC in Peyer’s patches of CD patients. Moreover, EGC mediators induced barrier dysfunction in CD patients. Thus, EGC might have harmful impacts on ongoing inflammation and contribute to the pathophysiology of the disease. |
| first_indexed | 2024-03-10T00:05:20Z |
| format | Article |
| id | doaj.art-7498af27187d40b4ab6ff61980dde0e1 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:05:20Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-7498af27187d40b4ab6ff61980dde0e12023-11-23T16:10:10ZengMDPI AGCells2073-44092022-01-0111333510.3390/cells11030335Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s DiseaseOlga Biskou0Felipe Meira de-Faria1Susanna M. Walter2Martin E. Winberg3Staffan Haapaniemi4Pär Myrelid5Johan D. Söderholm6Åsa V. Keita7Department of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Surgery, Vrinnevi Hospital, 60182 Norrköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenDepartment of Biomedical and Clinical Sciences, Linköping University, 58185 Linköping, SwedenEnteric glial cells (EGC) are known to regulate gastrointestinal functions; however, their role in Crohn’s disease (CD) is elusive. Microscopic erosions over the ileal Peyer’s patches are early signs of CD. The aim of this work was to assess the localization of EGC in the follicle and interfollicular region of the Peyer’s patches and in the lamina propria and study the effects of EGC mediators on barrier function in CD patients and non-inflammatory bowel disease (non-IBD) controls. EGC markers, glial fibrillary acidic protein (GFAP), and S100 calcium-binding protein β (S100β) were quantified by immunofluorescence and Western blotting. Both markers showed significantly more EGC in the Peyer’s patches and lamina propria of CD patients compared to the non-IBD controls. In CD patients there were significantly more EGC in Peyer’s patches compared to lamina propria, while the opposite pattern was seen in controls. Barrier function studies using Ussing chambers showed increased paracellular permeability by EGC mediators in CD patients, whereas permeability decreased by the mediators in controls. We show the accumulation of EGC in Peyer’s patches of CD patients. Moreover, EGC mediators induced barrier dysfunction in CD patients. Thus, EGC might have harmful impacts on ongoing inflammation and contribute to the pathophysiology of the disease.https://www.mdpi.com/2073-4409/11/3/335neuro-immune interactionsgut inflammationenteric nervous systemfollicle-associated epithelium |
| spellingShingle | Olga Biskou Felipe Meira de-Faria Susanna M. Walter Martin E. Winberg Staffan Haapaniemi Pär Myrelid Johan D. Söderholm Åsa V. Keita Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease Cells neuro-immune interactions gut inflammation enteric nervous system follicle-associated epithelium |
| title | Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease |
| title_full | Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease |
| title_fullStr | Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease |
| title_full_unstemmed | Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease |
| title_short | Increased Numbers of Enteric Glial Cells in the Peyer’s Patches and Enhanced Intestinal Permeability by Glial Cell Mediators in Patients with Ileal Crohn’s Disease |
| title_sort | increased numbers of enteric glial cells in the peyer s patches and enhanced intestinal permeability by glial cell mediators in patients with ileal crohn s disease |
| topic | neuro-immune interactions gut inflammation enteric nervous system follicle-associated epithelium |
| url | https://www.mdpi.com/2073-4409/11/3/335 |
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