Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide
QueF enzymes catalyze the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of the nitrile group of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) in the biosynthetic pathway to the tRNA modified nucleoside queuosine. The QueF-catalyzed reaction includes...
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MDPI AG
2017-03-01
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| Series: | Biomolecules |
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| Online Access: | http://www.mdpi.com/2218-273X/7/1/30 |
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| author | Adeba Mohammad Adriana Bon Ramos Bobby W. K. Lee Spencer W. Cohen Maryam K. Kiani Dirk Iwata-Reuyl Boguslaw Stec Manal A. Swairjo |
| author_facet | Adeba Mohammad Adriana Bon Ramos Bobby W. K. Lee Spencer W. Cohen Maryam K. Kiani Dirk Iwata-Reuyl Boguslaw Stec Manal A. Swairjo |
| author_sort | Adeba Mohammad |
| collection | DOAJ |
| description | QueF enzymes catalyze the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of the nitrile group of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) in the biosynthetic pathway to the tRNA modified nucleoside queuosine. The QueF-catalyzed reaction includes formation of a covalent thioimide intermediate with a conserved active site cysteine that is prone to oxidation in vivo. Here, we report the crystal structure of a mutant of Bacillus subtilis QueF, which reveals an unanticipated intramolecular disulfide formed between the catalytic Cys55 and a conserved Cys99 located near the active site. This structure is more symmetric than the substrate-bound structure and exhibits major rearrangement of the loops responsible for substrate binding. Mutation of Cys99 to Ala/Ser does not compromise enzyme activity, indicating that the disulfide does not play a catalytic role. Peroxide-induced inactivation of the wild-type enzyme is reversible with thioredoxin, while such inactivation of the Cys99Ala/Ser mutants is irreversible, consistent with protection of Cys55 from irreversible oxidation by disulfide formation with Cys99. Conservation of the cysteine pair, and the reported in vivo interaction of QueF with the thioredoxin-like hydroperoxide reductase AhpC in Escherichia coli suggest that regulation by the thioredoxin disulfide-thiol exchange system may constitute a general mechanism for protection of QueF from oxidative stress in vivo. |
| first_indexed | 2024-12-13T22:19:13Z |
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| id | doaj.art-7499677c37d347a3a4ca21b1160dcac0 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-13T22:19:13Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-7499677c37d347a3a4ca21b1160dcac02022-12-21T23:29:25ZengMDPI AGBiomolecules2218-273X2017-03-01713010.3390/biom7010030biom7010030Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular DisulfideAdeba Mohammad0Adriana Bon Ramos1Bobby W. K. Lee2Spencer W. Cohen3Maryam K. Kiani4Dirk Iwata-Reuyl5Boguslaw Stec6Manal A. Swairjo7Graduate College of Biomedical Sciences, Western University of Health Sciences, 309 E. Second Street, Pomona, CA 91766, USADepartment of Chemistry, Portland State University, P.O. Box 751, Portland, OR 97207, USADepartment of Chemistry, Portland State University, P.O. Box 751, Portland, OR 97207, USADepartment of Chemistry, Portland State University, P.O. Box 751, Portland, OR 97207, USAGraduate College of Biomedical Sciences, Western University of Health Sciences, 309 E. Second Street, Pomona, CA 91766, USADepartment of Chemistry, Portland State University, P.O. Box 751, Portland, OR 97207, USADepartment of Chemistry and Biochemistry, San Diego State University 5500 Campanile Drive, San Diego, CA 92182, USAGraduate College of Biomedical Sciences, Western University of Health Sciences, 309 E. Second Street, Pomona, CA 91766, USAQueF enzymes catalyze the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of the nitrile group of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) in the biosynthetic pathway to the tRNA modified nucleoside queuosine. The QueF-catalyzed reaction includes formation of a covalent thioimide intermediate with a conserved active site cysteine that is prone to oxidation in vivo. Here, we report the crystal structure of a mutant of Bacillus subtilis QueF, which reveals an unanticipated intramolecular disulfide formed between the catalytic Cys55 and a conserved Cys99 located near the active site. This structure is more symmetric than the substrate-bound structure and exhibits major rearrangement of the loops responsible for substrate binding. Mutation of Cys99 to Ala/Ser does not compromise enzyme activity, indicating that the disulfide does not play a catalytic role. Peroxide-induced inactivation of the wild-type enzyme is reversible with thioredoxin, while such inactivation of the Cys99Ala/Ser mutants is irreversible, consistent with protection of Cys55 from irreversible oxidation by disulfide formation with Cys99. Conservation of the cysteine pair, and the reported in vivo interaction of QueF with the thioredoxin-like hydroperoxide reductase AhpC in Escherichia coli suggest that regulation by the thioredoxin disulfide-thiol exchange system may constitute a general mechanism for protection of QueF from oxidative stress in vivo.http://www.mdpi.com/2218-273X/7/1/30tRNA modificationoxidoreductasetunneling fold |
| spellingShingle | Adeba Mohammad Adriana Bon Ramos Bobby W. K. Lee Spencer W. Cohen Maryam K. Kiani Dirk Iwata-Reuyl Boguslaw Stec Manal A. Swairjo Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide Biomolecules tRNA modification oxidoreductase tunneling fold |
| title | Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide |
| title_full | Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide |
| title_fullStr | Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide |
| title_full_unstemmed | Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide |
| title_short | Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide |
| title_sort | protection of the queuosine biosynthesis enzyme quef from irreversible oxidation by a conserved intramolecular disulfide |
| topic | tRNA modification oxidoreductase tunneling fold |
| url | http://www.mdpi.com/2218-273X/7/1/30 |
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