Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol
Abstract Background For decades, various cardiovascular symptoms have been relieved by the use of Ya-Hom Navakot, which is a formulation comprising 54 herbal medicines. The Thailand Ministry of Public Health listed Ya-Hom Navakot’s nine active principle and nomenclative herbal ingredients and termed...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2327-1 |
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| author | Napatara Tirawanchai Sudarat Supapornhemin Anchaleekorn Somkasetrin Bhoom Suktitipat Sumate Ampawong |
| author_facet | Napatara Tirawanchai Sudarat Supapornhemin Anchaleekorn Somkasetrin Bhoom Suktitipat Sumate Ampawong |
| author_sort | Napatara Tirawanchai |
| collection | DOAJ |
| description | Abstract Background For decades, various cardiovascular symptoms have been relieved by the use of Ya-Hom Navakot, which is a formulation comprising 54 herbal medicines. The Thailand Ministry of Public Health listed Ya-Hom Navakot’s nine active principle and nomenclative herbal ingredients and termed them ‘Phikud Navakot’ (PN). Several reports have confirmed that PN has cardiovascular benefits similar to Ya-Hom Navakot. However, whether PN facilitates lipid-lowering activity remains unclear. Methods The present study investigated an in vitro model for examining the gene expression levels of 3-hydroxyl-3-methylglutaryl-CoA reductase (HMGCR) and low-density lipoprotein receptor (LDL-R) in HepG2 cells using qRT-PCR. The ethanol and water extractions of Ya-Hom Navakot, PN and Ya-Hom Navakot without PN were compared. Results One mg/ml of both NYEF and NYWF were found to significantly lower cholesterol by either the up-regulation of LDL-R or down-regulation of HMGCR compared with negative controls and 1 mg/ml simvastatin (p < 0.05). PNEF also up-regulated LDL-R gene expression, even more than NYEF (p < 0.05). In addition, the ethanol and water extracts of PN significantly down-regulated HMGCR gene expression compared with those of Ya-Hom Navakot without PN (p < 0.05). Conclusion The use of Ya-Hom Navakot or PN may provide an alternative treatment to lower cholesterol through HMGCR gene inhibition and LDL-R gene enhancement. |
| first_indexed | 2024-12-14T21:00:53Z |
| format | Article |
| id | doaj.art-749bd705b22d4d81afa7c4993e10deea |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-14T21:00:53Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-749bd705b22d4d81afa7c4993e10deea2022-12-21T22:47:35ZengBMCBMC Complementary and Alternative Medicine1472-68822018-09-011811810.1186/s12906-018-2327-1Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterolNapatara Tirawanchai0Sudarat Supapornhemin1Anchaleekorn Somkasetrin2Bhoom Suktitipat3Sumate Ampawong4Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol UniversityAbstract Background For decades, various cardiovascular symptoms have been relieved by the use of Ya-Hom Navakot, which is a formulation comprising 54 herbal medicines. The Thailand Ministry of Public Health listed Ya-Hom Navakot’s nine active principle and nomenclative herbal ingredients and termed them ‘Phikud Navakot’ (PN). Several reports have confirmed that PN has cardiovascular benefits similar to Ya-Hom Navakot. However, whether PN facilitates lipid-lowering activity remains unclear. Methods The present study investigated an in vitro model for examining the gene expression levels of 3-hydroxyl-3-methylglutaryl-CoA reductase (HMGCR) and low-density lipoprotein receptor (LDL-R) in HepG2 cells using qRT-PCR. The ethanol and water extractions of Ya-Hom Navakot, PN and Ya-Hom Navakot without PN were compared. Results One mg/ml of both NYEF and NYWF were found to significantly lower cholesterol by either the up-regulation of LDL-R or down-regulation of HMGCR compared with negative controls and 1 mg/ml simvastatin (p < 0.05). PNEF also up-regulated LDL-R gene expression, even more than NYEF (p < 0.05). In addition, the ethanol and water extracts of PN significantly down-regulated HMGCR gene expression compared with those of Ya-Hom Navakot without PN (p < 0.05). Conclusion The use of Ya-Hom Navakot or PN may provide an alternative treatment to lower cholesterol through HMGCR gene inhibition and LDL-R gene enhancement.http://link.springer.com/article/10.1186/s12906-018-2327-1CholesterolHMG-CoA reductaseLow-density lipoprotein receptorPhikud NavakotYa-Hom Navakot |
| spellingShingle | Napatara Tirawanchai Sudarat Supapornhemin Anchaleekorn Somkasetrin Bhoom Suktitipat Sumate Ampawong Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol BMC Complementary and Alternative Medicine Cholesterol HMG-CoA reductase Low-density lipoprotein receptor Phikud Navakot Ya-Hom Navakot |
| title | Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol |
| title_full | Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol |
| title_fullStr | Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol |
| title_full_unstemmed | Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol |
| title_short | Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol |
| title_sort | regulatory effect of phikud navakot extract on hmg coa reductase and ldl r potential and alternate agents for lowering blood cholesterol |
| topic | Cholesterol HMG-CoA reductase Low-density lipoprotein receptor Phikud Navakot Ya-Hom Navakot |
| url | http://link.springer.com/article/10.1186/s12906-018-2327-1 |
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