Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy
Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing e...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/21/10151 |
| _version_ | 1797512783650095104 |
|---|---|
| author | Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li |
| author_facet | Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li |
| author_sort | Hung-Shih Lin |
| collection | DOAJ |
| description | Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing embryo during mouse pregnancy. Immunohistochemical staining was used to reveal the localization of QSOX1 protein, and HistoQuest was applied to quantify protein levels. The expression level of QSOX1 in the decidua and muscle cells of the pregnant uterus fluctuated dramatically during pregnancy. QSOX1 was ubiquitously expressed in the labyrinth, junction zone, and chorionic plate in the placenta. The quantitative analysis found that this protein was highly expressed in the spinal cord, lens, midbrain, cerebellum, medulla oblongata, and tooth of mouse embryos, followed by the heart, intercostal muscle, diaphragm, intermediate zone, extrinsic ocular muscle, spine, pons, epidermis, tongue, ganglion, vomeronasal organ, thoracic vertebrae, and thymus. Interestingly, QSOX1 was also markedly expressed in olfactory system tissues. This comprehensive spatiotemporal study of QSOX1 protein expression will provide a basis for further investigations of the QSOX1 physiological function in the pregnant uterus, placenta, and developing embryo. |
| first_indexed | 2024-03-10T06:06:34Z |
| format | Article |
| id | doaj.art-749e8c6f185647bbb513466b04ab8eb1 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T06:06:34Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-749e8c6f185647bbb513466b04ab8eb12023-11-22T20:28:35ZengMDPI AGApplied Sciences2076-34172021-10-0111211015110.3390/app112110151Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during PregnancyHung-Shih Lin0Robert Kuo-Kuang Lee1Tsung-Hsien Yang2Hsu-Wei Fang3Sheng-Hsiang Li4Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, TaiwanDepartment of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei 104, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Tamsui District, New Taipei 251, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Tamsui District, New Taipei 251, TaiwanQuiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing embryo during mouse pregnancy. Immunohistochemical staining was used to reveal the localization of QSOX1 protein, and HistoQuest was applied to quantify protein levels. The expression level of QSOX1 in the decidua and muscle cells of the pregnant uterus fluctuated dramatically during pregnancy. QSOX1 was ubiquitously expressed in the labyrinth, junction zone, and chorionic plate in the placenta. The quantitative analysis found that this protein was highly expressed in the spinal cord, lens, midbrain, cerebellum, medulla oblongata, and tooth of mouse embryos, followed by the heart, intercostal muscle, diaphragm, intermediate zone, extrinsic ocular muscle, spine, pons, epidermis, tongue, ganglion, vomeronasal organ, thoracic vertebrae, and thymus. Interestingly, QSOX1 was also markedly expressed in olfactory system tissues. This comprehensive spatiotemporal study of QSOX1 protein expression will provide a basis for further investigations of the QSOX1 physiological function in the pregnant uterus, placenta, and developing embryo.https://www.mdpi.com/2076-3417/11/21/10151quiescin sulfhydryl oxidase 1uterusplacentaembryomaternal-fetal interfacetissue distribution |
| spellingShingle | Hung-Shih Lin Robert Kuo-Kuang Lee Tsung-Hsien Yang Hsu-Wei Fang Sheng-Hsiang Li Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy Applied Sciences quiescin sulfhydryl oxidase 1 uterus placenta embryo maternal-fetal interface tissue distribution |
| title | Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy |
| title_full | Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy |
| title_fullStr | Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy |
| title_full_unstemmed | Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy |
| title_short | Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy |
| title_sort | spatiotemporal protein expression profiles of qsox1 in the murine uterus placenta and embryo during pregnancy |
| topic | quiescin sulfhydryl oxidase 1 uterus placenta embryo maternal-fetal interface tissue distribution |
| url | https://www.mdpi.com/2076-3417/11/21/10151 |
| work_keys_str_mv | AT hungshihlin spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT robertkuokuanglee spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT tsunghsienyang spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT hsuweifang spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy AT shenghsiangli spatiotemporalproteinexpressionprofilesofqsox1inthemurineuterusplacentaandembryoduringpregnancy |