DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium
Phalaenopsis-type Dendrobium is a popular orchid with good ornamental and market value. Despite their popularity, molecular regulation of anthocyanin biosynthesis during flower development remains poorly understood. In this study, we systematically investigated the regulatory roles of the transcript...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1046134/full |
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| author | Yachen Wang Hantai Yin Hantai Yin Zongxing Long Zongxing Long Wenjuan Zhu Junmei Yin Junmei Yin Xiqiang Song Chonghui Li Chonghui Li |
| author_facet | Yachen Wang Hantai Yin Hantai Yin Zongxing Long Zongxing Long Wenjuan Zhu Junmei Yin Junmei Yin Xiqiang Song Chonghui Li Chonghui Li |
| author_sort | Yachen Wang |
| collection | DOAJ |
| description | Phalaenopsis-type Dendrobium is a popular orchid with good ornamental and market value. Despite their popularity, molecular regulation of anthocyanin biosynthesis during flower development remains poorly understood. In this study, we systematically investigated the regulatory roles of the transcription factors DhMYB2 and DhbHLH1 in anthocyanins biosynthesis. Gene expression analyses indicated that both DhMYB2 and DhbHLH1 are specifically expressed in flowers and have similar expression patterns, showing high expression in purple floral tissues with anthocyanin accumulation. Transcriptomic analyses showed 29 differentially expressed genes corresponding to eight enzymes in anthocyanin biosynthesis pathway have similar expression patterns to DhMYB2 and DhbHLH1, with higher expression in the purple lips than the yellow petals and sepals of Dendrobium ‘Suriya Gold’. Further gene expression analyses and Pearson correlation matrix analyses of Dendrobium hybrid progenies revealed expression profiles of DhMYB2 and DhbHLH1 were positively correlated with the structural genes DhF3’H1, DhF3’5’H2, DhDFR, DhANS, and DhGT4. Yeast one-hybrid and dual‐luciferase reporter assays revealed DhMYB2 and DhbHLH1 can bind to promoter regions of DhF3’H1, DhF3’5’H2, DhDFR, DhANS and DhGT4, suggesting a role as transcriptional activators. These results provide new evidence of the molecular mechanisms of DhMYB2 and DhbHLH1 in anthocyanin biosynthesis in Phalaenopsis-type Dendrobium. |
| first_indexed | 2024-04-12T07:46:41Z |
| format | Article |
| id | doaj.art-749f12eeab9f45749a68b63bec9bf3e3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T07:46:41Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-749f12eeab9f45749a68b63bec9bf3e32022-12-22T03:41:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10461341046134DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type DendrobiumYachen Wang0Hantai Yin1Hantai Yin2Zongxing Long3Zongxing Long4Wenjuan Zhu5Junmei Yin6Junmei Yin7Xiqiang Song8Chonghui Li9Chonghui Li10Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), School of Forestry, School of Life Sciences, Hainan University, Haikou, ChinaHaikou Experimental Station, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, ChinaThe Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, ChinaHaikou Experimental Station, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, ChinaThe Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, ChinaKey Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), School of Forestry, School of Life Sciences, Hainan University, Haikou, ChinaHaikou Experimental Station, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, ChinaThe Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, ChinaKey Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants (Ministry of Education), School of Forestry, School of Life Sciences, Hainan University, Haikou, ChinaHaikou Experimental Station, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou, ChinaThe Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Danzhou, ChinaPhalaenopsis-type Dendrobium is a popular orchid with good ornamental and market value. Despite their popularity, molecular regulation of anthocyanin biosynthesis during flower development remains poorly understood. In this study, we systematically investigated the regulatory roles of the transcription factors DhMYB2 and DhbHLH1 in anthocyanins biosynthesis. Gene expression analyses indicated that both DhMYB2 and DhbHLH1 are specifically expressed in flowers and have similar expression patterns, showing high expression in purple floral tissues with anthocyanin accumulation. Transcriptomic analyses showed 29 differentially expressed genes corresponding to eight enzymes in anthocyanin biosynthesis pathway have similar expression patterns to DhMYB2 and DhbHLH1, with higher expression in the purple lips than the yellow petals and sepals of Dendrobium ‘Suriya Gold’. Further gene expression analyses and Pearson correlation matrix analyses of Dendrobium hybrid progenies revealed expression profiles of DhMYB2 and DhbHLH1 were positively correlated with the structural genes DhF3’H1, DhF3’5’H2, DhDFR, DhANS, and DhGT4. Yeast one-hybrid and dual‐luciferase reporter assays revealed DhMYB2 and DhbHLH1 can bind to promoter regions of DhF3’H1, DhF3’5’H2, DhDFR, DhANS and DhGT4, suggesting a role as transcriptional activators. These results provide new evidence of the molecular mechanisms of DhMYB2 and DhbHLH1 in anthocyanin biosynthesis in Phalaenopsis-type Dendrobium.https://www.frontiersin.org/articles/10.3389/fpls.2022.1046134/fullPhalaenopsis-type Dendrobiumfloral coloranthocyanin biosynthesisMYB transcription factorbHLH transcription factor |
| spellingShingle | Yachen Wang Hantai Yin Hantai Yin Zongxing Long Zongxing Long Wenjuan Zhu Junmei Yin Junmei Yin Xiqiang Song Chonghui Li Chonghui Li DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium Frontiers in Plant Science Phalaenopsis-type Dendrobium floral color anthocyanin biosynthesis MYB transcription factor bHLH transcription factor |
| title | DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium |
| title_full | DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium |
| title_fullStr | DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium |
| title_full_unstemmed | DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium |
| title_short | DhMYB2 and DhbHLH1 regulates anthocyanin accumulation via activation of late biosynthesis genes in Phalaenopsis-type Dendrobium |
| title_sort | dhmyb2 and dhbhlh1 regulates anthocyanin accumulation via activation of late biosynthesis genes in phalaenopsis type dendrobium |
| topic | Phalaenopsis-type Dendrobium floral color anthocyanin biosynthesis MYB transcription factor bHLH transcription factor |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1046134/full |
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