Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts
Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits prolif...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/2/309 |
| _version_ | 1797416061995319296 |
|---|---|
| author | Romina L. Ferrero Carmen Soto-Maldonado Caroline Weinstein-Oppenheimer Zaida Cabrera-Muñoz María Elvira Zúñiga-Hansen |
| author_facet | Romina L. Ferrero Carmen Soto-Maldonado Caroline Weinstein-Oppenheimer Zaida Cabrera-Muñoz María Elvira Zúñiga-Hansen |
| author_sort | Romina L. Ferrero |
| collection | DOAJ |
| description | Defatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent. |
| first_indexed | 2024-03-09T05:58:20Z |
| format | Article |
| id | doaj.art-74a5859bde164941a38122981128f0f9 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T05:58:20Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-74a5859bde164941a38122981128f0f92023-12-03T12:11:46ZengMDPI AGFoods2304-81582021-02-0110230910.3390/foods10020309Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human FibroblastsRomina L. Ferrero0Carmen Soto-Maldonado1Caroline Weinstein-Oppenheimer2Zaida Cabrera-Muñoz3María Elvira Zúñiga-Hansen4Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, ChileEscuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso 2360102, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, ChileEscuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, ChileDefatted rapeseed meal (DRM) is a sub-valorized agro-industrial by-product, with a high protein content whose peptides could have potential anticancer activity against cancer cell lines. The objective of the present study is to obtain an enzymatic hydrolysate of rapeseed protein that inhibits proliferation on a breast cancer cell line (MCF-7), but not healthy human fibroblast cells. The DRM was solubilized in an alkaline medium to obtain an alkaline rapeseed extract (RAE). Acid precipitation of the proteins contained in RAE recovered a rapeseed protein isolate (RPI). To produce protein hydrolysates, two alkaline protease and different enzyme/substrate ratios were used. All the protein hydrolysates showed antiproliferative activity on MCF-7 cells. However, only the hydrolysate recovered from the enzymatic hydrolysis of RPI (Degree of hydrolysis (DH ) between 8.5 and 9% (DH1)) did not affect human fibroblast cells, inhibiting 83.9% of MCF-7 cells’ proliferation and showing a mass yield of 22.9% (based on the initial DRM). The SDS-PAGE gel revealed that DH1 was composed mainly of 10 kDa peptides and, to a lesser extent, 5 and 2 kDa. It is concluded that DH1 is a promising peptide extract for future research as a putative anti-breast cancer agent.https://www.mdpi.com/2304-8158/10/2/309bioactive peptidesantiproliferative activitybreast canceranticancermeal rapeseed |
| spellingShingle | Romina L. Ferrero Carmen Soto-Maldonado Caroline Weinstein-Oppenheimer Zaida Cabrera-Muñoz María Elvira Zúñiga-Hansen Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts Foods bioactive peptides antiproliferative activity breast cancer anticancer meal rapeseed |
| title | Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts |
| title_full | Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts |
| title_fullStr | Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts |
| title_full_unstemmed | Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts |
| title_short | Antiproliferative Rapeseed Defatted Meal Protein and Their Hydrolysates on MCF-7 Breast Cancer Cells and Human Fibroblasts |
| title_sort | antiproliferative rapeseed defatted meal protein and their hydrolysates on mcf 7 breast cancer cells and human fibroblasts |
| topic | bioactive peptides antiproliferative activity breast cancer anticancer meal rapeseed |
| url | https://www.mdpi.com/2304-8158/10/2/309 |
| work_keys_str_mv | AT rominalferrero antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts AT carmensotomaldonado antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts AT carolineweinsteinoppenheimer antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts AT zaidacabreramunoz antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts AT mariaelvirazunigahansen antiproliferativerapeseeddefattedmealproteinandtheirhydrolysatesonmcf7breastcancercellsandhumanfibroblasts |