Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function
Summary: Very-long-chain polyunsaturated fatty acids (VLCPUFAs; C24-38) constitute a unique class of PUFA that have important biological roles, but the lack of a suitable dietary source has limited research in this field. We produced an n-3 C24-28-rich VLCPUFA-oil concentrated from fish oil to study...
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223024884 |
| _version_ | 1797388745307062272 |
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| author | Zhi-Hong Yang Aruna Gorusupudi Todd A. Lydic Anupam K. Mondal Seizo Sato Isao Yamazaki Hideaki Yamaguchi Jingrong Tang Krishna Vamsi Rojulpote Anna B. Lin Hannah Decot Hannah Koch Daniel C. Brock Ranganathan Arunkumar Zhen-Dan Shi Zu-Xi Yu Milton Pryor Julia F. Kun Rolf E. Swenson Anand Swaroop Paul S. Bernstein Alan T. Remaley |
| author_facet | Zhi-Hong Yang Aruna Gorusupudi Todd A. Lydic Anupam K. Mondal Seizo Sato Isao Yamazaki Hideaki Yamaguchi Jingrong Tang Krishna Vamsi Rojulpote Anna B. Lin Hannah Decot Hannah Koch Daniel C. Brock Ranganathan Arunkumar Zhen-Dan Shi Zu-Xi Yu Milton Pryor Julia F. Kun Rolf E. Swenson Anand Swaroop Paul S. Bernstein Alan T. Remaley |
| author_sort | Zhi-Hong Yang |
| collection | DOAJ |
| description | Summary: Very-long-chain polyunsaturated fatty acids (VLCPUFAs; C24-38) constitute a unique class of PUFA that have important biological roles, but the lack of a suitable dietary source has limited research in this field. We produced an n-3 C24-28-rich VLCPUFA-oil concentrated from fish oil to study its bioavailability and physiological functions in C57BL/6J mice. The serum and retinal C24:5 levels increased significantly compared to control after a single-dose gavage, and VLCPUFAs were incorporated into the liver, brain, and eyes after 8-week supplementation. Dietary VLCPUFAs resulted in favorable cardiometabolic changes, and improved electroretinography responses and visual performance. VLCPUFA supplementation changed the expression of genes involved in PPAR signaling pathways. Further in vitro studies demonstrated that the VLCPUFA-oil and chemically synthesized C24:5 are potent agonists for PPARs. The multiple potential beneficial effects of fish oil-derived VLCPUFAs on cardiometabolic risk and eye health in mice support future efforts to develop VLCPUFA-oil into a supplemental therapy. |
| first_indexed | 2024-03-08T22:45:16Z |
| format | Article |
| id | doaj.art-74a7d220a4254a238343bbd4e714de34 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:45:16Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-74a7d220a4254a238343bbd4e714de342023-12-17T06:40:41ZengElsevieriScience2589-00422023-12-012612108411Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal functionZhi-Hong Yang0Aruna Gorusupudi1Todd A. Lydic2Anupam K. Mondal3Seizo Sato4Isao Yamazaki5Hideaki Yamaguchi6Jingrong Tang7Krishna Vamsi Rojulpote8Anna B. Lin9Hannah Decot10Hannah Koch11Daniel C. Brock12Ranganathan Arunkumar13Zhen-Dan Shi14Zu-Xi Yu15Milton Pryor16Julia F. Kun17Rolf E. Swenson18Anand Swaroop19Paul S. Bernstein20Alan T. Remaley21Lipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USADepartment of Ophthalmology and Visual Sciences, John A. Moran Eye Center, Salt Lake City, UT 84132, USADepartment of Physiology, Collaborative Mass Spectrometry Core, Michigan State University, East Lansing, MI 48824, USANeurobiology, Neurodegeneration and Repair Laboratory, National Eye Institute, NIH, Bethesda, MD 20892, USACentral Research Laboratory, Nissui Corporation, 1-32-3 Nanakuni, Hachioji, Tokyo 192-0991, JapanCentral Research Laboratory, Nissui Corporation, 1-32-3 Nanakuni, Hachioji, Tokyo 192-0991, JapanCentral Research Laboratory, Nissui Corporation, 1-32-3 Nanakuni, Hachioji, Tokyo 192-0991, JapanLipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USALipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USADepartment of Physiology, Collaborative Mass Spectrometry Core, Michigan State University, East Lansing, MI 48824, USADepartment of Physiology, Collaborative Mass Spectrometry Core, Michigan State University, East Lansing, MI 48824, USADepartment of Physiology, Collaborative Mass Spectrometry Core, Michigan State University, East Lansing, MI 48824, USANeurobiology, Neurodegeneration and Repair Laboratory, National Eye Institute, NIH, Bethesda, MD 20892, USADepartment of Ophthalmology and Visual Sciences, John A. Moran Eye Center, Salt Lake City, UT 84132, USAChemistry and Synthesis Center, NHLBI, NIH, Bethesda, MD 20892, USAPathology Core, NHLBI, NIH, Bethesda, MD 20892, USALipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USALipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USAChemistry and Synthesis Center, NHLBI, NIH, Bethesda, MD 20892, USANeurobiology, Neurodegeneration and Repair Laboratory, National Eye Institute, NIH, Bethesda, MD 20892, USADepartment of Ophthalmology and Visual Sciences, John A. Moran Eye Center, Salt Lake City, UT 84132, USA; Corresponding authorLipoprotein Metabolism Section, Translational Vascular Medicine Branch, National Heart, Lung and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Corresponding authorSummary: Very-long-chain polyunsaturated fatty acids (VLCPUFAs; C24-38) constitute a unique class of PUFA that have important biological roles, but the lack of a suitable dietary source has limited research in this field. We produced an n-3 C24-28-rich VLCPUFA-oil concentrated from fish oil to study its bioavailability and physiological functions in C57BL/6J mice. The serum and retinal C24:5 levels increased significantly compared to control after a single-dose gavage, and VLCPUFAs were incorporated into the liver, brain, and eyes after 8-week supplementation. Dietary VLCPUFAs resulted in favorable cardiometabolic changes, and improved electroretinography responses and visual performance. VLCPUFA supplementation changed the expression of genes involved in PPAR signaling pathways. Further in vitro studies demonstrated that the VLCPUFA-oil and chemically synthesized C24:5 are potent agonists for PPARs. The multiple potential beneficial effects of fish oil-derived VLCPUFAs on cardiometabolic risk and eye health in mice support future efforts to develop VLCPUFA-oil into a supplemental therapy.http://www.sciencedirect.com/science/article/pii/S2589004223024884Dietary supplementPhysiologyCell biology |
| spellingShingle | Zhi-Hong Yang Aruna Gorusupudi Todd A. Lydic Anupam K. Mondal Seizo Sato Isao Yamazaki Hideaki Yamaguchi Jingrong Tang Krishna Vamsi Rojulpote Anna B. Lin Hannah Decot Hannah Koch Daniel C. Brock Ranganathan Arunkumar Zhen-Dan Shi Zu-Xi Yu Milton Pryor Julia F. Kun Rolf E. Swenson Anand Swaroop Paul S. Bernstein Alan T. Remaley Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function iScience Dietary supplement Physiology Cell biology |
| title | Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| title_full | Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| title_fullStr | Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| title_full_unstemmed | Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| title_short | Dietary fish oil enriched in very-long-chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| title_sort | dietary fish oil enriched in very long chain polyunsaturated fatty acid reduces cardiometabolic risk factors and improves retinal function |
| topic | Dietary supplement Physiology Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223024884 |
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