Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents
Recently, there have been urgent economic and scientific demands to decrease the use of chemical fungicides during the treatment of phytopathogens, due to their human health and environmental impacts. This study explored the biocontrol efficacy of novel and eco-friendly preen (uropygial) oil and end...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2737 |
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| author | Elhagag A. Hassan Yasser S. Mostafa Saad Alamri Mohamed Hashem Nivien A. Nafady |
| author_facet | Elhagag A. Hassan Yasser S. Mostafa Saad Alamri Mohamed Hashem Nivien A. Nafady |
| author_sort | Elhagag A. Hassan |
| collection | DOAJ |
| description | Recently, there have been urgent economic and scientific demands to decrease the use of chemical fungicides during the treatment of phytopathogens, due to their human health and environmental impacts. This study explored the biocontrol efficacy of novel and eco-friendly preen (uropygial) oil and endophytic <i>Bacillus safensis</i> in managing postharvest <i>Botrytis</i> grey mold in strawberry fruit. The preen oil (25 μL/mL) showed high antifungal activity against <i>B</i>. <i>cinerea</i> Str5 in terms of the reduction in the fungal radial growth (41.3%) and the fungal colony-forming units (28.6%) compared to the control. A new strain of <i>Bacillus safensis</i> B3 had a good potential to produce chitinase enzymes (3.69 ± 0.31 U/mL), hydrolytic lipase (10.65 ± 0.51 U/mL), and protease enzymes (13.28 ± 0.65 U/mL), which are responsible for the hydrolysis of the <i>B</i>. <i>cinerea</i> Str5 cell wall and, consequently, restrict fungal growth. The in vivo experiment on strawberry fruit showed that preen (uropygial) oil reduced the disease severity by 87.25%, while the endophytic bacteria <i>B</i>. <i>safensis</i> B3 reduced it by 86.52%. This study reports the efficiency of individually applied bioagents in the control of phytopathogenic fungi for the first time and, consequently, encourages their application as a new and innovative strategy for prospective agricultural technology and food safety. |
| first_indexed | 2024-03-10T03:16:34Z |
| format | Article |
| id | doaj.art-74aa367a87e8441f8a043b8ea1a95ce8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:16:34Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-74aa367a87e8441f8a043b8ea1a95ce82023-11-23T10:12:26ZengMDPI AGPlants2223-77472021-12-011012273710.3390/plants10122737Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel BioagentsElhagag A. Hassan0Yasser S. Mostafa1Saad Alamri2Mohamed Hashem3Nivien A. Nafady4Botany Department, Faculty of Science, Assiut University, Assiut 71516, EgyptBiology Department, Faculty of Science, King Khalid University, P.O. Box 10255, Abha 61321, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, P.O. Box 10255, Abha 61321, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, P.O. Box 10255, Abha 61321, Saudi ArabiaBotany Department, Faculty of Science, Assiut University, Assiut 71516, EgyptRecently, there have been urgent economic and scientific demands to decrease the use of chemical fungicides during the treatment of phytopathogens, due to their human health and environmental impacts. This study explored the biocontrol efficacy of novel and eco-friendly preen (uropygial) oil and endophytic <i>Bacillus safensis</i> in managing postharvest <i>Botrytis</i> grey mold in strawberry fruit. The preen oil (25 μL/mL) showed high antifungal activity against <i>B</i>. <i>cinerea</i> Str5 in terms of the reduction in the fungal radial growth (41.3%) and the fungal colony-forming units (28.6%) compared to the control. A new strain of <i>Bacillus safensis</i> B3 had a good potential to produce chitinase enzymes (3.69 ± 0.31 U/mL), hydrolytic lipase (10.65 ± 0.51 U/mL), and protease enzymes (13.28 ± 0.65 U/mL), which are responsible for the hydrolysis of the <i>B</i>. <i>cinerea</i> Str5 cell wall and, consequently, restrict fungal growth. The in vivo experiment on strawberry fruit showed that preen (uropygial) oil reduced the disease severity by 87.25%, while the endophytic bacteria <i>B</i>. <i>safensis</i> B3 reduced it by 86.52%. This study reports the efficiency of individually applied bioagents in the control of phytopathogenic fungi for the first time and, consequently, encourages their application as a new and innovative strategy for prospective agricultural technology and food safety.https://www.mdpi.com/2223-7747/10/12/2737<i>Botrytis cinerea</i><i>Bacillus safensis</i>preen (uropygail) oilstrawberrypostharvest diseaseinnovative strategy |
| spellingShingle | Elhagag A. Hassan Yasser S. Mostafa Saad Alamri Mohamed Hashem Nivien A. Nafady Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents Plants <i>Botrytis cinerea</i> <i>Bacillus safensis</i> preen (uropygail) oil strawberry postharvest disease innovative strategy |
| title | Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents |
| title_full | Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents |
| title_fullStr | Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents |
| title_full_unstemmed | Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents |
| title_short | Biosafe Management of <i>Botrytis</i> Grey Mold of Strawberry Fruit by Novel Bioagents |
| title_sort | biosafe management of i botrytis i grey mold of strawberry fruit by novel bioagents |
| topic | <i>Botrytis cinerea</i> <i>Bacillus safensis</i> preen (uropygail) oil strawberry postharvest disease innovative strategy |
| url | https://www.mdpi.com/2223-7747/10/12/2737 |
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