KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo
Summary: Kinesin 1 (KIF5) is one major type of motor protein in neurons, but its members’ function in the intact brain remains less studied. Using in vivo two-photon imaging, we find that conditional knockout of Kif5b (KIF5B cKO) in CaMKIIα-Cre-expressing neurons shows heightened turnover and lower...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724002341 |
| _version_ | 1797268980412448768 |
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| author | Albert Hiu Ka Fok Yuhua Huang Beth Wing Lam So Qiyu Zheng Chun Sing Carlos Tse Xiaoyang Li Kenneth Kin-Yip Wong Jiandong Huang Kwok-On Lai Cora Sau Wan Lai |
| author_facet | Albert Hiu Ka Fok Yuhua Huang Beth Wing Lam So Qiyu Zheng Chun Sing Carlos Tse Xiaoyang Li Kenneth Kin-Yip Wong Jiandong Huang Kwok-On Lai Cora Sau Wan Lai |
| author_sort | Albert Hiu Ka Fok |
| collection | DOAJ |
| description | Summary: Kinesin 1 (KIF5) is one major type of motor protein in neurons, but its members’ function in the intact brain remains less studied. Using in vivo two-photon imaging, we find that conditional knockout of Kif5b (KIF5B cKO) in CaMKIIα-Cre-expressing neurons shows heightened turnover and lower stability of dendritic spines in layer 2/3 pyramidal neurons with reduced spine postsynaptic density protein 95 acquisition in the mouse cortex. Furthermore, the RNA-binding protein fragile X mental retardation protein (FMRP) is translocated to the proximity of newly formed spines several hours before the spine formation events in vivo in control mice, but this preceding transport of FMRP is abolished in KIF5B cKO mice. We further find that FMRP is localized closer to newly formed spines after fear extinction, but this learning-dependent localization is disrupted in KIF5B cKO mice. Our findings provide the crucial in vivo evidence that KIF5B is involved in the dendritic targeting of synaptic proteins that underlies dendritic spine plasticity. |
| first_indexed | 2024-04-25T01:41:06Z |
| format | Article |
| id | doaj.art-74aa4e2ce3e544d9814de6b1a079c9d3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-25T01:41:06Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-74aa4e2ce3e544d9814de6b1a079c9d32024-03-08T05:18:34ZengElsevierCell Reports2211-12472024-03-01433113906KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivoAlbert Hiu Ka Fok0Yuhua Huang1Beth Wing Lam So2Qiyu Zheng3Chun Sing Carlos Tse4Xiaoyang Li5Kenneth Kin-Yip Wong6Jiandong Huang7Kwok-On Lai8Cora Sau Wan Lai9School of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China; Advanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, ChinaAdvanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, New Territories, Hong Kong SAR, China; Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China; Chinese Academy of Sciences (CAS) Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China; Clinical Oncology Center, Shenzhen Key Laboratory for Cancer Metastasis and Personalized Therapy, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China; Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen University, Guangzhou, China; State Key Laboratory of Cognitive and Brain Research, The University of Hong Kong, Hong Kong SAR, ChinaDepartment of Neuroscience, City University of Hong Kong, Hong Kong SAR, China; Hong Kong Institute for Advanced Study, City University of Hong Kong, Hong Kong SAR, China; Corresponding authorSchool of Biomedical Sciences, The University of Hong Kong, Hong Kong SAR, China; State Key Laboratory of Cognitive and Brain Research, The University of Hong Kong, Hong Kong SAR, China; Corresponding authorSummary: Kinesin 1 (KIF5) is one major type of motor protein in neurons, but its members’ function in the intact brain remains less studied. Using in vivo two-photon imaging, we find that conditional knockout of Kif5b (KIF5B cKO) in CaMKIIα-Cre-expressing neurons shows heightened turnover and lower stability of dendritic spines in layer 2/3 pyramidal neurons with reduced spine postsynaptic density protein 95 acquisition in the mouse cortex. Furthermore, the RNA-binding protein fragile X mental retardation protein (FMRP) is translocated to the proximity of newly formed spines several hours before the spine formation events in vivo in control mice, but this preceding transport of FMRP is abolished in KIF5B cKO mice. We further find that FMRP is localized closer to newly formed spines after fear extinction, but this learning-dependent localization is disrupted in KIF5B cKO mice. Our findings provide the crucial in vivo evidence that KIF5B is involved in the dendritic targeting of synaptic proteins that underlies dendritic spine plasticity.http://www.sciencedirect.com/science/article/pii/S2211124724002341CP: Neuroscience |
| spellingShingle | Albert Hiu Ka Fok Yuhua Huang Beth Wing Lam So Qiyu Zheng Chun Sing Carlos Tse Xiaoyang Li Kenneth Kin-Yip Wong Jiandong Huang Kwok-On Lai Cora Sau Wan Lai KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo Cell Reports CP: Neuroscience |
| title | KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo |
| title_full | KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo |
| title_fullStr | KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo |
| title_full_unstemmed | KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo |
| title_short | KIF5B plays important roles in dendritic spine plasticity and dendritic localization of PSD95 and FMRP in the mouse cortex in vivo |
| title_sort | kif5b plays important roles in dendritic spine plasticity and dendritic localization of psd95 and fmrp in the mouse cortex in vivo |
| topic | CP: Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724002341 |
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