Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage
The purple tubers of <i>Dioscorea alata</i> L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of <i>Dioscorea alata</i> L. and its crude extracts on cells prior...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/8/1401 |
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| author | Yanhong Yun Huiyu Shi Yanyu Wang Fengyuan Yang Yuanxin Zhang Haibo Feng Junpu Chen Xuemei Wang |
| author_facet | Yanhong Yun Huiyu Shi Yanyu Wang Fengyuan Yang Yuanxin Zhang Haibo Feng Junpu Chen Xuemei Wang |
| author_sort | Yanhong Yun |
| collection | DOAJ |
| description | The purple tubers of <i>Dioscorea alata</i> L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of <i>Dioscorea alata</i> L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H<sub>2</sub>O<sub>2</sub> as the oxidant. Specifically, when the concentration of H<sub>2</sub>O<sub>2</sub> was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of <i>Dioscorea alata</i> L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P<sub>X</sub>) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, <i>Dioscorea alata</i> L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL. |
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| language | English |
| last_indexed | 2024-03-11T05:18:57Z |
| publishDate | 2023-04-01 |
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| series | Animals |
| spelling | doaj.art-74ac53fd82bd4a3b8e47a8bfc2f2f20b2023-11-17T18:01:18ZengMDPI AGAnimals2076-26152023-04-01138140110.3390/ani13081401Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative DamageYanhong Yun0Huiyu Shi1Yanyu Wang2Fengyuan Yang3Yuanxin Zhang4Haibo Feng5Junpu Chen6Xuemei Wang7College of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaCollege of Animal Science and Technology, Hainan University, Haikou 570228, ChinaThe purple tubers of <i>Dioscorea alata</i> L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of <i>Dioscorea alata</i> L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H<sub>2</sub>O<sub>2</sub> as the oxidant. Specifically, when the concentration of H<sub>2</sub>O<sub>2</sub> was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of <i>Dioscorea alata</i> L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P<sub>X</sub>) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, <i>Dioscorea alata</i> L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL.https://www.mdpi.com/2076-2615/13/8/1401<i>Dioscorea alata</i> L.proanthocyanidinsanthocyaninsIPEC-J2 cellsH<sub>2</sub>O<sub>2</sub>pre-protection |
| spellingShingle | Yanhong Yun Huiyu Shi Yanyu Wang Fengyuan Yang Yuanxin Zhang Haibo Feng Junpu Chen Xuemei Wang Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage Animals <i>Dioscorea alata</i> L. proanthocyanidins anthocyanins IPEC-J2 cells H<sub>2</sub>O<sub>2</sub> pre-protection |
| title | Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage |
| title_full | Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage |
| title_fullStr | Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage |
| title_full_unstemmed | Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage |
| title_short | Pre-Protection and Mechanism of Crude Extracts from <i>Dioscorea alata</i> L. on H<sub>2</sub>O<sub>2</sub>-Induced IPEC-J2 Cells Oxidative Damage |
| title_sort | pre protection and mechanism of crude extracts from i dioscorea alata i l on h sub 2 sub o sub 2 sub induced ipec j2 cells oxidative damage |
| topic | <i>Dioscorea alata</i> L. proanthocyanidins anthocyanins IPEC-J2 cells H<sub>2</sub>O<sub>2</sub> pre-protection |
| url | https://www.mdpi.com/2076-2615/13/8/1401 |
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