Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis
Background: Chemokine receptors have been shown to play an important role in the development and metastatic spread of various malignancies. In this study, the gene expression profile of some key chemokine receptors involved in metastasis has been investigated in esophageal and breast cancer cell lin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Iranian Journal of Public Health |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/4999 |
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| author | Mohammad Amir MISHAN Asieh HEIRANI-TABASI Neda MOKHBERIAN Malihe HASSANZADE Hamid KALALIAN MOGHADDAM Ahmad Reza BAHRAMI Naghmeh AHMADIANKIA |
| author_facet | Mohammad Amir MISHAN Asieh HEIRANI-TABASI Neda MOKHBERIAN Malihe HASSANZADE Hamid KALALIAN MOGHADDAM Ahmad Reza BAHRAMI Naghmeh AHMADIANKIA |
| author_sort | Mohammad Amir MISHAN |
| collection | DOAJ |
| description | Background: Chemokine receptors have been shown to play an important role in the development and metastatic spread of various malignancies. In this study, the gene expression profile of some key chemokine receptors involved in metastasis has been investigated in esophageal and breast cancer cell lines.
Methods: In a descriptive study, gene expression profile of CCR1, CCR6, CCR7, CCR9, CXCR1, and CXCR4 in human esophageal cancer cell line (KYSE-30) and human breast cancer cell line (MCF7) were analyzed using real-time PCR and their results were compared accordingly.
Results: We demonstrated for the first time the expression of CCR1, CCR6, CCR7, CCR9, CXCR1, and CXCR4 at transcriptional level in human esophageal cancer cell line. The expression of CCR1, CCR7 and CXCR4 were lower in esophageal compared with breast cancer cells, although without significant difference. CCR9 was highly expressed in esophageal cancer cells as compared to the breast cancer cells (P < 0.05). Similarly, the expression of CCR6 and CXCR1 were higher, although without significant difference.
Conclusion: Esophageal cancer cells like breast cancer express some key chemokine receptors involved in metastasis. Targeting of proposed receptors in esophageal cancer may be a novel strategy for prevention of cancer metastasis.
Keywords: Esophageal cancer, Breast cancer, Metastasis, Chemokine receptors, Targeted cancer therapy |
| first_indexed | 2024-12-19T14:46:20Z |
| format | Article |
| id | doaj.art-74acca83eca04813a9ba2619800ef761 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-19T14:46:20Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-74acca83eca04813a9ba2619800ef7612022-12-21T20:16:56ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932015-10-0144104715Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into MetastasisMohammad Amir MISHANAsieh HEIRANI-TABASINeda MOKHBERIANMalihe HASSANZADEHamid KALALIAN MOGHADDAMAhmad Reza BAHRAMINaghmeh AHMADIANKIABackground: Chemokine receptors have been shown to play an important role in the development and metastatic spread of various malignancies. In this study, the gene expression profile of some key chemokine receptors involved in metastasis has been investigated in esophageal and breast cancer cell lines. Methods: In a descriptive study, gene expression profile of CCR1, CCR6, CCR7, CCR9, CXCR1, and CXCR4 in human esophageal cancer cell line (KYSE-30) and human breast cancer cell line (MCF7) were analyzed using real-time PCR and their results were compared accordingly. Results: We demonstrated for the first time the expression of CCR1, CCR6, CCR7, CCR9, CXCR1, and CXCR4 at transcriptional level in human esophageal cancer cell line. The expression of CCR1, CCR7 and CXCR4 were lower in esophageal compared with breast cancer cells, although without significant difference. CCR9 was highly expressed in esophageal cancer cells as compared to the breast cancer cells (P < 0.05). Similarly, the expression of CCR6 and CXCR1 were higher, although without significant difference. Conclusion: Esophageal cancer cells like breast cancer express some key chemokine receptors involved in metastasis. Targeting of proposed receptors in esophageal cancer may be a novel strategy for prevention of cancer metastasis. Keywords: Esophageal cancer, Breast cancer, Metastasis, Chemokine receptors, Targeted cancer therapyhttps://ijph.tums.ac.ir/index.php/ijph/article/view/4999 |
| spellingShingle | Mohammad Amir MISHAN Asieh HEIRANI-TABASI Neda MOKHBERIAN Malihe HASSANZADE Hamid KALALIAN MOGHADDAM Ahmad Reza BAHRAMI Naghmeh AHMADIANKIA Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis Iranian Journal of Public Health |
| title | Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis |
| title_full | Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis |
| title_fullStr | Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis |
| title_full_unstemmed | Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis |
| title_short | Analysis of Chemokine Receptor Gene Expression in Esophageal Cancer Cells Compared with Breast Cancer with Insights into Metastasis |
| title_sort | analysis of chemokine receptor gene expression in esophageal cancer cells compared with breast cancer with insights into metastasis |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/4999 |
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