Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study
(1) Background: The present pilot in vitro study evaluated, physically and biologically, the effects produced by the wear of the titanium surface using different drill models. (2) Methods: Titanium disks were subjected to wear using four different burs and accordingly divided into the following test...
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/15/7920 |
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| author | Sergio Alexandre Gehrke Berenice Anina Dedavid Germán Odella Colla Piedad N. De Aza Leticia Pérez-Díaz |
| author_facet | Sergio Alexandre Gehrke Berenice Anina Dedavid Germán Odella Colla Piedad N. De Aza Leticia Pérez-Díaz |
| author_sort | Sergio Alexandre Gehrke |
| collection | DOAJ |
| description | (1) Background: The present pilot in vitro study evaluated, physically and biologically, the effects produced by the wear of the titanium surface using different drill models. (2) Methods: Titanium disks were subjected to wear using four different burs and accordingly divided into the following test groups (<i>n</i> = 12 disks per group): Tungsten Burs (TB group), Tungsten Carbide Burs (TCB group), Coarse-Grained Diamond Burs (CGB group), and Fine-Grained Diamond Burs (FGB group). As a control group (CON group), titanium disks with a smooth surface (machined) were used. The samples were subjected to atomic force microscopy (AFM), profilometry analysis, scanning electron microscopy (SEM), and cell viability and adhesion assessments. (3) Results: The values of the measured roughness parameters showed statistical differences among the five groups (<i>p</i> = 0.0035 for Ra and <i>p</i> = 0.0010 for Rz). All test groups showed an important difference statistically (<i>p</i> = 0.0032) to the CON group for the cell viability and adhesion analysis. The data of cell absorbance at 570 nm were 0.4122 ± 0.05 for the CON group, 0.1354 ± 0.02 for the TB group, 0.123 ± 0.01 for the TCB group, 0.1414 ± 0.02 for the CGB group, and 0.1216 ± 0.03 for the FGB group. Additionally, the cell count showed the following adherence percentages: 57.6 ± 4.6% for the CON group, 22.9 ± 3.3% for the TB group, 23.4 ± 2.9% for the TCB group, 22.5 ± 3.1% for the CGB group, and 23.7 ± 3.3% for the FGB group. However, no statistical differences were found among the four test groups analyzed (<i>p</i> = 0.3916). (4) Conclusions: The results showed that the changes produced on the surface by the four different bur models altered the topography characteristics and affected the cell viability and adhesion in comparison with the control group. |
| first_indexed | 2024-03-09T12:45:53Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T12:45:53Z |
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| spelling | doaj.art-74ae852c11fc4e858e29ce4b97a6875d2023-11-30T22:12:24ZengMDPI AGApplied Sciences2076-34172022-08-011215792010.3390/app12157920Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental StudySergio Alexandre Gehrke0Berenice Anina Dedavid1Germán Odella Colla2Piedad N. De Aza3Leticia Pérez-Díaz4Bioface Institute, Postgrado em Odontologia UCAM, Montevideo 11100, UruguayDepartment of Materials Engineering, Pontificial Catholic University of Rio Grande do Sul, Porto Alegre 90619-900, BrazilBioface Institute, Postgrado em Odontologia UCAM, Montevideo 11100, UruguayInstituto de Bioingenieria, Universidad Miguel Hernández, Avda. Ferrocarril s/n., 03202 Elche, SpainLaboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la Republica, Montevideo 11400, Uruguay(1) Background: The present pilot in vitro study evaluated, physically and biologically, the effects produced by the wear of the titanium surface using different drill models. (2) Methods: Titanium disks were subjected to wear using four different burs and accordingly divided into the following test groups (<i>n</i> = 12 disks per group): Tungsten Burs (TB group), Tungsten Carbide Burs (TCB group), Coarse-Grained Diamond Burs (CGB group), and Fine-Grained Diamond Burs (FGB group). As a control group (CON group), titanium disks with a smooth surface (machined) were used. The samples were subjected to atomic force microscopy (AFM), profilometry analysis, scanning electron microscopy (SEM), and cell viability and adhesion assessments. (3) Results: The values of the measured roughness parameters showed statistical differences among the five groups (<i>p</i> = 0.0035 for Ra and <i>p</i> = 0.0010 for Rz). All test groups showed an important difference statistically (<i>p</i> = 0.0032) to the CON group for the cell viability and adhesion analysis. The data of cell absorbance at 570 nm were 0.4122 ± 0.05 for the CON group, 0.1354 ± 0.02 for the TB group, 0.123 ± 0.01 for the TCB group, 0.1414 ± 0.02 for the CGB group, and 0.1216 ± 0.03 for the FGB group. Additionally, the cell count showed the following adherence percentages: 57.6 ± 4.6% for the CON group, 22.9 ± 3.3% for the TB group, 23.4 ± 2.9% for the TCB group, 22.5 ± 3.1% for the CGB group, and 23.7 ± 3.3% for the FGB group. However, no statistical differences were found among the four test groups analyzed (<i>p</i> = 0.3916). (4) Conclusions: The results showed that the changes produced on the surface by the four different bur models altered the topography characteristics and affected the cell viability and adhesion in comparison with the control group.https://www.mdpi.com/2076-3417/12/15/7920titanium surfaceimplantoplastycell viability and adhesionperi-implantitistitanium instrumentationfibroblast cells |
| spellingShingle | Sergio Alexandre Gehrke Berenice Anina Dedavid Germán Odella Colla Piedad N. De Aza Leticia Pérez-Díaz Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study Applied Sciences titanium surface implantoplasty cell viability and adhesion peri-implantitis titanium instrumentation fibroblast cells |
| title | Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study |
| title_full | Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study |
| title_fullStr | Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study |
| title_full_unstemmed | Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study |
| title_short | Titanium Surface Analysis after Instrumentation with Different Burs Simulating the Implantoplasty Technique: A Pilot In Vitro Experimental Study |
| title_sort | titanium surface analysis after instrumentation with different burs simulating the implantoplasty technique a pilot in vitro experimental study |
| topic | titanium surface implantoplasty cell viability and adhesion peri-implantitis titanium instrumentation fibroblast cells |
| url | https://www.mdpi.com/2076-3417/12/15/7920 |
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