Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em>
Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of &...
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MDPI AG
2012-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/13/6/7648 |
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| author | Carlos M. Silva Armando J. D. Silvestre Ricardo M. Couto Carlos P. Neto Pedro C. Simões Carmen S. R. Freire Eduardo L. G. Oliveira Rui M. A. Domingues |
| author_facet | Carlos M. Silva Armando J. D. Silvestre Ricardo M. Couto Carlos P. Neto Pedro C. Simões Carmen S. R. Freire Eduardo L. G. Oliveira Rui M. A. Domingues |
| author_sort | Carlos M. Silva |
| collection | DOAJ |
| description | Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of <em>Eucalyptus</em> <em>globulus</em> deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by<strong> </strong>several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold. |
| first_indexed | 2024-04-12T09:36:47Z |
| format | Article |
| id | doaj.art-74af61451628470ab9d62e59bb01031b |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T09:36:47Z |
| publishDate | 2012-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-74af61451628470ab9d62e59bb01031b2022-12-22T03:38:13ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-06-011367648766210.3390/ijms13067648Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em>Carlos M. SilvaArmando J. D. SilvestreRicardo M. CoutoCarlos P. NetoPedro C. SimõesCarmen S. R. FreireEduardo L. G. OliveiraRui M. A. DominguesEucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of <em>Eucalyptus</em> <em>globulus</em> deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by<strong> </strong>several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.http://www.mdpi.com/1422-0067/13/6/7648<em>Eucalyptus globulus</em>barktriterpenoidstriterpenic acidssupercritical fluid extractioncarbon dioxidebiorefinery |
| spellingShingle | Carlos M. Silva Armando J. D. Silvestre Ricardo M. Couto Carlos P. Neto Pedro C. Simões Carmen S. R. Freire Eduardo L. G. Oliveira Rui M. A. Domingues Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> International Journal of Molecular Sciences <em>Eucalyptus globulus</em> bark triterpenoids triterpenic acids supercritical fluid extraction carbon dioxide biorefinery |
| title | Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> |
| title_full | Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> |
| title_fullStr | Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> |
| title_full_unstemmed | Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> |
| title_short | Supercritical Fluid Extraction of <em>Eucalyptus globulus</em> Bark—A Promising Approach for Triterpenoid Production<em></em> |
| title_sort | supercritical fluid extraction of lt em gt eucalyptus globulus lt em gt bark a promising approach for triterpenoid production lt em gt lt em gt |
| topic | <em>Eucalyptus globulus</em> bark triterpenoids triterpenic acids supercritical fluid extraction carbon dioxide biorefinery |
| url | http://www.mdpi.com/1422-0067/13/6/7648 |
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