Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the al...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721005785 |
| _version_ | 1819099699882229760 |
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| author | Elisa Boutet-Robinet Maria M. Haykal Saleha Hashim Teresa Frisan Océane C.B. Martin |
| author_facet | Elisa Boutet-Robinet Maria M. Haykal Saleha Hashim Teresa Frisan Océane C.B. Martin |
| author_sort | Elisa Boutet-Robinet |
| collection | DOAJ |
| description | Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]).For complete details on the use and execution of this protocol, please refer to Martin et al. (2021). |
| first_indexed | 2024-12-22T00:51:02Z |
| format | Article |
| id | doaj.art-74af7b9dad9c425286389f75f326f812 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:51:02Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-74af7b9dad9c425286389f75f326f8122022-12-21T18:44:26ZengElsevierSTAR Protocols2666-16672021-12-0124100872Detection of DNA damage by alkaline comet assay in mouse colonic mucosaElisa Boutet-Robinet0Maria M. Haykal1Saleha Hashim2Teresa Frisan3Océane C.B. Martin4Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, FranceINSERM U981, LabEx LERMIT, Université Paris 12 Sud, Gustave Roussy Research Center, Department of Molecular Medicine, Villejuif, FranceToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, FranceDepartment of Molecular Biology, Umeå University, Umeå, Sweden; Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, SwedenUniversity of Bordeaux, INSERM, BaRITOn, U1053, 33000 Bordeaux, France; Corresponding authorSummary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe the specific steps for assessing DNA damage by the alkaline comet assay of colonic mucosal samples. The description of the comet assay protocol follows the international guidelines (Minimum Information for Reporting on the Comet Assay [Moller et al., 2020]).For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721005785Cell isolationCell-based AssaysMicroscopy |
| spellingShingle | Elisa Boutet-Robinet Maria M. Haykal Saleha Hashim Teresa Frisan Océane C.B. Martin Detection of DNA damage by alkaline comet assay in mouse colonic mucosa STAR Protocols Cell isolation Cell-based Assays Microscopy |
| title | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
| title_full | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
| title_fullStr | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
| title_full_unstemmed | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
| title_short | Detection of DNA damage by alkaline comet assay in mouse colonic mucosa |
| title_sort | detection of dna damage by alkaline comet assay in mouse colonic mucosa |
| topic | Cell isolation Cell-based Assays Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721005785 |
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