Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases
Respiratory diseases remain a major health concern worldwide because they subject patients to considerable financial and psychosocial burdens and result in a high rate of morbidity and mortality. Although significant progress has been made in understanding the underlying pathologic mechanisms of sev...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1067422/full |
| _version_ | 1797869978181959680 |
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| author | Miquéias Lopes-Pacheco Patricia R. M. Rocco |
| author_facet | Miquéias Lopes-Pacheco Patricia R. M. Rocco |
| author_sort | Miquéias Lopes-Pacheco |
| collection | DOAJ |
| description | Respiratory diseases remain a major health concern worldwide because they subject patients to considerable financial and psychosocial burdens and result in a high rate of morbidity and mortality. Although significant progress has been made in understanding the underlying pathologic mechanisms of severe respiratory diseases, most therapies are supportive, aiming to mitigate symptoms and slow down their progressive course but cannot improve lung function or reverse tissue remodeling. Mesenchymal stromal cells (MSCs) are at the forefront of the regenerative medicine field due to their unique biomedical potential in promoting immunomodulation, anti-inflammatory, anti-apoptotic and antimicrobial activities, and tissue repair in various experimental models. However, despite several years of preclinical research on MSCs, therapeutic outcomes have fallen far short in early-stage clinical trials for respiratory diseases. This limited efficacy has been associated with several factors, such as reduced MSC homing, survival, and infusion in the late course of lung disease. Accordingly, genetic engineering and preconditioning methods have emerged as functional enhancement strategies to potentiate the therapeutic actions of MSCs and thus achieve better clinical outcomes. This narrative review describes various strategies that have been investigated in the experimental setting to functionally potentiate the therapeutic properties of MSCs for respiratory diseases. These include changes in culture conditions, exposure of MSCs to inflammatory environments, pharmacological agents or other substances, and genetic manipulation for enhanced and sustained expression of genes of interest. Future directions and challenges in efficiently translating MSC research into clinical practice are discussed. |
| first_indexed | 2024-04-10T00:20:05Z |
| format | Article |
| id | doaj.art-74b3b73afd7c4b349299b3c62d3e7798 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T00:20:05Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-74b3b73afd7c4b349299b3c62d3e77982023-03-16T04:25:25ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-03-011410.3389/fphar.2023.10674221067422Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseasesMiquéias Lopes-Pacheco0Patricia R. M. Rocco1Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Lisbon, PortugalLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, BrazilRespiratory diseases remain a major health concern worldwide because they subject patients to considerable financial and psychosocial burdens and result in a high rate of morbidity and mortality. Although significant progress has been made in understanding the underlying pathologic mechanisms of severe respiratory diseases, most therapies are supportive, aiming to mitigate symptoms and slow down their progressive course but cannot improve lung function or reverse tissue remodeling. Mesenchymal stromal cells (MSCs) are at the forefront of the regenerative medicine field due to their unique biomedical potential in promoting immunomodulation, anti-inflammatory, anti-apoptotic and antimicrobial activities, and tissue repair in various experimental models. However, despite several years of preclinical research on MSCs, therapeutic outcomes have fallen far short in early-stage clinical trials for respiratory diseases. This limited efficacy has been associated with several factors, such as reduced MSC homing, survival, and infusion in the late course of lung disease. Accordingly, genetic engineering and preconditioning methods have emerged as functional enhancement strategies to potentiate the therapeutic actions of MSCs and thus achieve better clinical outcomes. This narrative review describes various strategies that have been investigated in the experimental setting to functionally potentiate the therapeutic properties of MSCs for respiratory diseases. These include changes in culture conditions, exposure of MSCs to inflammatory environments, pharmacological agents or other substances, and genetic manipulation for enhanced and sustained expression of genes of interest. Future directions and challenges in efficiently translating MSC research into clinical practice are discussed.https://www.frontiersin.org/articles/10.3389/fphar.2023.1067422/fullcell therapygenetic engineeringimmunomodulationinflammationlungpreconditioning |
| spellingShingle | Miquéias Lopes-Pacheco Patricia R. M. Rocco Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases Frontiers in Pharmacology cell therapy genetic engineering immunomodulation inflammation lung preconditioning |
| title | Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| title_full | Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| title_fullStr | Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| title_full_unstemmed | Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| title_short | Functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| title_sort | functional enhancement strategies to potentiate the therapeutic properties of mesenchymal stromal cells for respiratory diseases |
| topic | cell therapy genetic engineering immunomodulation inflammation lung preconditioning |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1067422/full |
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