Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits
This study aimed to investigate the effect of feeding insoluble fiber on the microbiota and metabolites of the caecum and feces of rabbits recovering from epizootic rabbit enteropathy relative to non-infected rabbits. Rabbits that had either recovered from epizootic rabbit enteropathy or ones that h...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/5/571 |
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| author | Xiao-Haitzi Daniel Puón-Peláez Neil Ross McEwan Roberto Carlos Álvarez-Martínez Gerardo Mariscal-Landín Gerardo Manuel Nava-Morales Juan Mosqueda Andrea Margarita Olvera-Ramírez |
| author_facet | Xiao-Haitzi Daniel Puón-Peláez Neil Ross McEwan Roberto Carlos Álvarez-Martínez Gerardo Mariscal-Landín Gerardo Manuel Nava-Morales Juan Mosqueda Andrea Margarita Olvera-Ramírez |
| author_sort | Xiao-Haitzi Daniel Puón-Peláez |
| collection | DOAJ |
| description | This study aimed to investigate the effect of feeding insoluble fiber on the microbiota and metabolites of the caecum and feces of rabbits recovering from epizootic rabbit enteropathy relative to non-infected rabbits. Rabbits that had either recovered from epizootic rabbit enteropathy or ones that had never had epizootic rabbit enteropathy were fed on a diet of 32% or 36% neutral detergent fiber until they were 70 days of age. At this point, the short-chain fatty acid and ammonia levels were measured in caecotroph and fecal samples and compared using 2 × 2 ANOVA. The microbial composition of the samples was also analyzed using next-generation sequencing and compared by PERMANOVA. Caecotrophic samples from previously affected rabbits on lower fiber diets had higher short-chain fatty acid contents and higher species diversity index values for some indices (<i>p</i> < 0.05), although the fecal samples showed lower species diversity levels (<i>p</i> < 0.05). In addition, the PERMANOVA analyses demonstrated that differences were detected in the microbial composition of both fecal and caecotrophic samples, depending on the disease status at the outset of the experiment (<i>p</i> < 0.05). The results of this work show that, although there is some potential in the use of high-fiber diets for the treatment of rabbits that have had epizootic rabbit enteropathy, they are not able to produce the same digestive tract properties as those seen in rabbits that have never had the condition. This is true even after the rabbits have recovered from epizootic rabbit enteropathy. |
| first_indexed | 2024-03-10T03:11:28Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:11:28Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-74b58da493114ed787c964fb59ed4fb32023-11-23T12:32:45ZengMDPI AGPathogens2076-08172022-05-0111557110.3390/pathogens11050571Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected RabbitsXiao-Haitzi Daniel Puón-Peláez0Neil Ross McEwan1Roberto Carlos Álvarez-Martínez2Gerardo Mariscal-Landín3Gerardo Manuel Nava-Morales4Juan Mosqueda5Andrea Margarita Olvera-Ramírez6Doctorado en Ciencias Biológicas, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, Querétaro C.P. 76230, MexicoSchool of Pharmacy & Life Sciences, Robert Gordon University, Garthdee House, Garthdee Rd, Aberdeen AB10 7GJ, UKFacultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av. Junipero Serra, Antiguo Aeropuerto, Campus Aeropuerto S/N, Santiago de Querétaro, Querétaro C.P. 76140, MexicoCentro Nacional de Investigación en Fisiología Animal INIFAP, Km. 1, Carretera a Colón, Col. Ajuchitlán Colón, Querétaro C.P. 76280, MexicoFacultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Santiago de Querétaro, Querétaro C.P. 76010, MexicoCuerpo Académico Salud Animal y Microbiología Ambiental, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, Querétaro C.P. 76230, MexicoCuerpo Académico Salud Animal y Microbiología Ambiental, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias S/N, Juriquilla, Delegación Santa Rosa Jáuregui, Querétaro C.P. 76230, MexicoThis study aimed to investigate the effect of feeding insoluble fiber on the microbiota and metabolites of the caecum and feces of rabbits recovering from epizootic rabbit enteropathy relative to non-infected rabbits. Rabbits that had either recovered from epizootic rabbit enteropathy or ones that had never had epizootic rabbit enteropathy were fed on a diet of 32% or 36% neutral detergent fiber until they were 70 days of age. At this point, the short-chain fatty acid and ammonia levels were measured in caecotroph and fecal samples and compared using 2 × 2 ANOVA. The microbial composition of the samples was also analyzed using next-generation sequencing and compared by PERMANOVA. Caecotrophic samples from previously affected rabbits on lower fiber diets had higher short-chain fatty acid contents and higher species diversity index values for some indices (<i>p</i> < 0.05), although the fecal samples showed lower species diversity levels (<i>p</i> < 0.05). In addition, the PERMANOVA analyses demonstrated that differences were detected in the microbial composition of both fecal and caecotrophic samples, depending on the disease status at the outset of the experiment (<i>p</i> < 0.05). The results of this work show that, although there is some potential in the use of high-fiber diets for the treatment of rabbits that have had epizootic rabbit enteropathy, they are not able to produce the same digestive tract properties as those seen in rabbits that have never had the condition. This is true even after the rabbits have recovered from epizootic rabbit enteropathy.https://www.mdpi.com/2076-0817/11/5/571rabbitenteropathyepizooticfiberfermentation |
| spellingShingle | Xiao-Haitzi Daniel Puón-Peláez Neil Ross McEwan Roberto Carlos Álvarez-Martínez Gerardo Mariscal-Landín Gerardo Manuel Nava-Morales Juan Mosqueda Andrea Margarita Olvera-Ramírez Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits Pathogens rabbit enteropathy epizootic fiber fermentation |
| title | Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits |
| title_full | Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits |
| title_fullStr | Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits |
| title_full_unstemmed | Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits |
| title_short | Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits |
| title_sort | effect of feeding insoluble fiber on the microbiota and metabolites of the caecum and feces of rabbits recovering from epizootic rabbit enteropathy relative to non infected rabbits |
| topic | rabbit enteropathy epizootic fiber fermentation |
| url | https://www.mdpi.com/2076-0817/11/5/571 |
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