Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil
Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1996-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000200003 |
| _version_ | 1797702974377558016 |
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| author | Rita CN Cubel Aparecida GP Garcia Claudia S Pegado Hilda I Ramos Maria EF Fonseca Jonhatan P Clewley Bernard J Cohen Jussara P Nascimento |
| author_facet | Rita CN Cubel Aparecida GP Garcia Claudia S Pegado Hilda I Ramos Maria EF Fonseca Jonhatan P Clewley Bernard J Cohen Jussara P Nascimento |
| author_sort | Rita CN Cubel |
| collection | DOAJ |
| description | Formalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1982, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease. |
| first_indexed | 2024-03-12T04:58:44Z |
| format | Article |
| id | doaj.art-74b620f3be20465083063a7a8cefce1b |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:44Z |
| publishDate | 1996-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-74b620f3be20465083063a7a8cefce1b2023-09-03T09:14:35ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601996-04-0191214715110.1590/S0074-02761996000200003Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, BrazilRita CN CubelAparecida GP GarciaClaudia S PegadoHilda I RamosMaria EF FonsecaJonhatan P ClewleyBernard J CohenJussara P NascimentoFormalin-fixed paraffin embedded lung and liver tissue from 23 cases of non immune hydrops fetalis and five control cases, in which hydrops were due to syphilis (3) and genetic causes (2), were examined for the presence of human parvovirus B19 by DNA hybridisation. Using in situ hybridisation with a biotynilated probe one positive case was detected. Using 32P-labelled probes in a dot blot assay format, five further positives were obtained. These were all confirmed as positive by a nested polymerase chain reaction assay. Electron microscopy revealed virus in all these five positive cases. The six B19 DNA positive cases of hydrops fetalis were from 1974, 1980, 1982, 1987 and 1988, four of which occurred during the second half of the year, confirming the seasonality of the disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000200003human parvovirus B19hydrops fetalisvirus detection |
| spellingShingle | Rita CN Cubel Aparecida GP Garcia Claudia S Pegado Hilda I Ramos Maria EF Fonseca Jonhatan P Clewley Bernard J Cohen Jussara P Nascimento Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil Memorias do Instituto Oswaldo Cruz human parvovirus B19 hydrops fetalis virus detection |
| title | Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil |
| title_full | Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil |
| title_fullStr | Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil |
| title_full_unstemmed | Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil |
| title_short | Human parvovirus B19 infection and hydrops fetalis in Rio de Janeiro, Brazil |
| title_sort | human parvovirus b19 infection and hydrops fetalis in rio de janeiro brazil |
| topic | human parvovirus B19 hydrops fetalis virus detection |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000200003 |
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