IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES
This study explored the impact of supplementation of L- carnitine on the performance of broiler chicken, biochemical parameters and expression of cationic amino acid transporter (CAT2), myogenic determining factor (MYOD) and myogenic factor 5 (MYF5) genes. This study included two groups; the first...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2019-03-01
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| Series: | Slovenian Veterinary Research |
| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/805 |
| _version_ | 1827294446139473920 |
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| author | Tarek Abouzed Doaa Dorghamm Khaled Kahilo Azza Elkattawy Eldsokey Nassef Hanan El-sawy |
| author_facet | Tarek Abouzed Doaa Dorghamm Khaled Kahilo Azza Elkattawy Eldsokey Nassef Hanan El-sawy |
| author_sort | Tarek Abouzed |
| collection | DOAJ |
| description |
This study explored the impact of supplementation of L- carnitine on the performance of broiler chicken, biochemical parameters and expression of cationic amino acid transporter (CAT2), myogenic determining factor (MYOD) and myogenic factor 5 (MYF5) genes. This study included two groups; the first one is the control group and the second one received L-carnitine at a dose 50 mg/kg/day in drinking water. After 35 day, the sera and muscle tissue were obtained for biochemical and real time polymerase chain reaction (qPCR) analysis. The obtained results revealed a significant increase in the body weight, total body weight gain and a significant decrease in food consumption, food conversion rate in L-carnitine-supplemented group as compared to the control group. Moreover, there was no significant changes in serum cholesterol, triacylglycerol, AST, ALT, total protein and uric acid levels which showed a slight decrease in L-carnitine-supplemented group in comparison with the control group. This study also revealed a significant up regulation for the expression of CAT2, MYOD and MYF5 genes in muscles of broiler chicken-supplemented with L- carnitine as compared to the control one. The present study concluded that supplementation of L- carnitine to broiler chicken was useful to increase the total body weight gain and muscle mass and this beneficial effect was associated with up regulation of CAT2, MYOD, and MYF5 genes.
Key words: L-carnitine; broiler chicken; growth traits; CAT2; MYOD; MYF5
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| first_indexed | 2024-04-24T14:02:16Z |
| format | Article |
| id | doaj.art-74b740886ab94d7aa5015c9b137ce96e |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:16Z |
| publishDate | 2019-03-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-74b740886ab94d7aa5015c9b137ce96e2024-04-03T12:39:21ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612019-03-015622-Suppl10.26873/SVR-805-2019IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENESTarek AbouzedDoaa DorghammKhaled KahiloAzza ElkattawyEldsokey NassefHanan El-sawy This study explored the impact of supplementation of L- carnitine on the performance of broiler chicken, biochemical parameters and expression of cationic amino acid transporter (CAT2), myogenic determining factor (MYOD) and myogenic factor 5 (MYF5) genes. This study included two groups; the first one is the control group and the second one received L-carnitine at a dose 50 mg/kg/day in drinking water. After 35 day, the sera and muscle tissue were obtained for biochemical and real time polymerase chain reaction (qPCR) analysis. The obtained results revealed a significant increase in the body weight, total body weight gain and a significant decrease in food consumption, food conversion rate in L-carnitine-supplemented group as compared to the control group. Moreover, there was no significant changes in serum cholesterol, triacylglycerol, AST, ALT, total protein and uric acid levels which showed a slight decrease in L-carnitine-supplemented group in comparison with the control group. This study also revealed a significant up regulation for the expression of CAT2, MYOD and MYF5 genes in muscles of broiler chicken-supplemented with L- carnitine as compared to the control one. The present study concluded that supplementation of L- carnitine to broiler chicken was useful to increase the total body weight gain and muscle mass and this beneficial effect was associated with up regulation of CAT2, MYOD, and MYF5 genes. Key words: L-carnitine; broiler chicken; growth traits; CAT2; MYOD; MYF5 https://www.slovetres.si/index.php/SVR/article/view/805 |
| spellingShingle | Tarek Abouzed Doaa Dorghamm Khaled Kahilo Azza Elkattawy Eldsokey Nassef Hanan El-sawy IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES Slovenian Veterinary Research |
| title | IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES |
| title_full | IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES |
| title_fullStr | IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES |
| title_full_unstemmed | IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES |
| title_short | IMPACT OF L-CARNITINE SUPPLEMENTATION ON GROWTH OF BROILER CHICKEN THROUGH DETERMINATION OF CHANGES IN THE EXPRESSION OF CAT2, MYOD AND MYF5 GENES |
| title_sort | impact of l carnitine supplementation on growth of broiler chicken through determination of changes in the expression of cat2 myod and myf5 genes |
| url | https://www.slovetres.si/index.php/SVR/article/view/805 |
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