Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis
Renal cell carcinoma (RCC) originates from the epithelial cells of the renal tubules and has a high degree of malignancy and heterogeneity. Recent studies have found that exosomes regulate intercellular communication via transferring various bioactive molecules, such as circular RNAs (circRNAs), whi...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/1/123 |
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| author | Yiguan Qian Yang Li Luwei Xu Ke Chen Ning Liu Xiaobing Yang Qian Lv Rongfei Li Changcheng Zhou Zheng Xu Ruipeng Jia Yu-Zheng Ge |
| author_facet | Yiguan Qian Yang Li Luwei Xu Ke Chen Ning Liu Xiaobing Yang Qian Lv Rongfei Li Changcheng Zhou Zheng Xu Ruipeng Jia Yu-Zheng Ge |
| author_sort | Yiguan Qian |
| collection | DOAJ |
| description | Renal cell carcinoma (RCC) originates from the epithelial cells of the renal tubules and has a high degree of malignancy and heterogeneity. Recent studies have found that exosomes regulate intercellular communication via transferring various bioactive molecules, such as circular RNAs (circRNAs), which are critical for cancer progression. However, the role of tumor cell-derived exosomal circRNAs in RCC remains unclear. In this study, we reported the high expression of circ-PRKCI in RCC tissues and serum exosomes. We also found that circ-PRKCI could be transferred exosomally from highly malignant RCC cells to relatively less malignant RCC cells. Tumor cell-derived exosomal circ-PRKCI promoted the proliferation, migration, and invasion of RCC cells, while inhibiting their apoptosis. Mechanistically, we found that circ-PRKCI promoted the proliferation of RCC via the miR-545-3p/CCND1 signaling pathway. Our study is the first to report the potential mechanisms of tumor cell-derived exosomal circ-PRKCI in RCC. In conclusion, this study will provide a new understanding about the molecular mechanisms of RCC progression. |
| first_indexed | 2024-03-11T10:06:52Z |
| format | Article |
| id | doaj.art-74b8564ac934434fa459ba3b56860330 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T10:06:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-74b8564ac934434fa459ba3b568603302023-11-16T15:01:56ZengMDPI AGCancers2072-66942022-12-0115112310.3390/cancers15010123Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 AxisYiguan Qian0Yang Li1Luwei Xu2Ke Chen3Ning Liu4Xiaobing Yang5Qian Lv6Rongfei Li7Changcheng Zhou8Zheng Xu9Ruipeng Jia10Yu-Zheng Ge11Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaDepartment of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210012, ChinaRenal cell carcinoma (RCC) originates from the epithelial cells of the renal tubules and has a high degree of malignancy and heterogeneity. Recent studies have found that exosomes regulate intercellular communication via transferring various bioactive molecules, such as circular RNAs (circRNAs), which are critical for cancer progression. However, the role of tumor cell-derived exosomal circRNAs in RCC remains unclear. In this study, we reported the high expression of circ-PRKCI in RCC tissues and serum exosomes. We also found that circ-PRKCI could be transferred exosomally from highly malignant RCC cells to relatively less malignant RCC cells. Tumor cell-derived exosomal circ-PRKCI promoted the proliferation, migration, and invasion of RCC cells, while inhibiting their apoptosis. Mechanistically, we found that circ-PRKCI promoted the proliferation of RCC via the miR-545-3p/CCND1 signaling pathway. Our study is the first to report the potential mechanisms of tumor cell-derived exosomal circ-PRKCI in RCC. In conclusion, this study will provide a new understanding about the molecular mechanisms of RCC progression.https://www.mdpi.com/2072-6694/15/1/123renal cell carcinomaexosomecirc-PRKCImicroRNA-545-3pcyclin D1proliferation |
| spellingShingle | Yiguan Qian Yang Li Luwei Xu Ke Chen Ning Liu Xiaobing Yang Qian Lv Rongfei Li Changcheng Zhou Zheng Xu Ruipeng Jia Yu-Zheng Ge Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis Cancers renal cell carcinoma exosome circ-PRKCI microRNA-545-3p cyclin D1 proliferation |
| title | Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis |
| title_full | Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis |
| title_fullStr | Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis |
| title_full_unstemmed | Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis |
| title_short | Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis |
| title_sort | tumor cell derived exosomal circ prkci promotes proliferation of renal cell carcinoma via regulating mir 545 3p ccnd1 axis |
| topic | renal cell carcinoma exosome circ-PRKCI microRNA-545-3p cyclin D1 proliferation |
| url | https://www.mdpi.com/2072-6694/15/1/123 |
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