AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens
Quorum sensing (QS) is a signaling mechanism governed by bacteria used to converse at inter- and intra-species levels through small self-produced chemicals called N-acylhomoserine lactones (AHLs). Through QS, bacteria regulate and organize the virulence factors’ production, including biofilm formati...
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.634593/full |
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| author | Issac Abraham Sybiya Vasantha Packiavathy Arunachalam Kannappan Arunachalam Kannappan Sivaprakasam Thiyagarajan Ramanathan Srinivasan Ramanathan Srinivasan Ramanathan Srinivasan Danaraj Jeyapragash John Bosco John Paul Pazhanivel Velmurugan Pazhanivel Velmurugan Arumugam Veera Ravi |
| author_facet | Issac Abraham Sybiya Vasantha Packiavathy Arunachalam Kannappan Arunachalam Kannappan Sivaprakasam Thiyagarajan Ramanathan Srinivasan Ramanathan Srinivasan Ramanathan Srinivasan Danaraj Jeyapragash John Bosco John Paul Pazhanivel Velmurugan Pazhanivel Velmurugan Arumugam Veera Ravi |
| author_sort | Issac Abraham Sybiya Vasantha Packiavathy |
| collection | DOAJ |
| description | Quorum sensing (QS) is a signaling mechanism governed by bacteria used to converse at inter- and intra-species levels through small self-produced chemicals called N-acylhomoserine lactones (AHLs). Through QS, bacteria regulate and organize the virulence factors’ production, including biofilm formation. AHLs can be degraded by an action called quorum quenching (QQ) and hence QQ strategy can effectively be employed to combat biofilm-associated bacterial pathogenesis. The present study aimed to identify novel bacterial species with QQ potential. Screening of Palk Bay marine sediment bacteria for QQ activity ended up with the identification of marine bacterial isolate 28 (MSB-28), which exhibited a profound QQ activity against QS biomarker strain Chromobacterium violaceum ATCC 12472. The isolate MSB-28 was identified as Psychrobacter sp. through 16S-rRNA sequencing. Psychrobacter sp. also demonstrated a pronounced activity in controlling the biofilm formation in different bacteria and biofilm-associated virulence factors’ production in P. aeruginosa PAO1. Solvent extraction, heat inactivation, and proteinase K treatment assays clearly evidence the enzymatic nature of the bioactive lead. Furthermore, AHL’s lactone ring cleavage was confirmed with experiments including ring closure assay and chromatographic analysis, and thus the AHL-lactonase enzyme production in Psychrobacter sp. To conclude, this is the first report stating the AHL-lactonase mediated QQ activity from marine sediment bacteria Psychrobacter sp. Future work deals with the characterization, purification, and mass cultivation of the purified protein and should pave the way to assessing the feasibility of the identified protein in controlling QS and biofilm-mediated multidrug resistant bacterial infections in mono or multi-species conditions. |
| first_indexed | 2024-12-14T15:27:59Z |
| format | Article |
| id | doaj.art-74b9107c5120496da00f83f10758b231 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T15:27:59Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-74b9107c5120496da00f83f10758b2312022-12-21T22:55:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-04-011210.3389/fmicb.2021.634593634593AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial PathogensIssac Abraham Sybiya Vasantha Packiavathy0Arunachalam Kannappan1Arunachalam Kannappan2Sivaprakasam Thiyagarajan3Ramanathan Srinivasan4Ramanathan Srinivasan5Ramanathan Srinivasan6Danaraj Jeyapragash7John Bosco John Paul8Pazhanivel Velmurugan9Pazhanivel Velmurugan10Arumugam Veera Ravi11Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, IndiaDepartment of Biotechnology, Alagappa University, Karaikudi, IndiaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, IndiaDepartment of Biotechnology, Alagappa University, Karaikudi, IndiaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou, ChinaDepartment of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, IndiaDepartment of Electronics and Communication Engineering, Karunya Institute of Technology and Sciences, Coimbatore, IndiaDepartment of Biotechnology, Alagappa University, Karaikudi, IndiaCentre for Materials Engineering and Regnerative Medicine, Bharath Institute of Higher Education and Research, Chennai, IndiaDepartment of Biotechnology, Alagappa University, Karaikudi, IndiaQuorum sensing (QS) is a signaling mechanism governed by bacteria used to converse at inter- and intra-species levels through small self-produced chemicals called N-acylhomoserine lactones (AHLs). Through QS, bacteria regulate and organize the virulence factors’ production, including biofilm formation. AHLs can be degraded by an action called quorum quenching (QQ) and hence QQ strategy can effectively be employed to combat biofilm-associated bacterial pathogenesis. The present study aimed to identify novel bacterial species with QQ potential. Screening of Palk Bay marine sediment bacteria for QQ activity ended up with the identification of marine bacterial isolate 28 (MSB-28), which exhibited a profound QQ activity against QS biomarker strain Chromobacterium violaceum ATCC 12472. The isolate MSB-28 was identified as Psychrobacter sp. through 16S-rRNA sequencing. Psychrobacter sp. also demonstrated a pronounced activity in controlling the biofilm formation in different bacteria and biofilm-associated virulence factors’ production in P. aeruginosa PAO1. Solvent extraction, heat inactivation, and proteinase K treatment assays clearly evidence the enzymatic nature of the bioactive lead. Furthermore, AHL’s lactone ring cleavage was confirmed with experiments including ring closure assay and chromatographic analysis, and thus the AHL-lactonase enzyme production in Psychrobacter sp. To conclude, this is the first report stating the AHL-lactonase mediated QQ activity from marine sediment bacteria Psychrobacter sp. Future work deals with the characterization, purification, and mass cultivation of the purified protein and should pave the way to assessing the feasibility of the identified protein in controlling QS and biofilm-mediated multidrug resistant bacterial infections in mono or multi-species conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2021.634593/fullAHL-lactonasemarine Palk Bay sedimentPseudomonas aeruginosaPsychrobacter sp.quorum quenching |
| spellingShingle | Issac Abraham Sybiya Vasantha Packiavathy Arunachalam Kannappan Arunachalam Kannappan Sivaprakasam Thiyagarajan Ramanathan Srinivasan Ramanathan Srinivasan Ramanathan Srinivasan Danaraj Jeyapragash John Bosco John Paul Pazhanivel Velmurugan Pazhanivel Velmurugan Arumugam Veera Ravi AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens Frontiers in Microbiology AHL-lactonase marine Palk Bay sediment Pseudomonas aeruginosa Psychrobacter sp. quorum quenching |
| title | AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens |
| title_full | AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens |
| title_fullStr | AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens |
| title_full_unstemmed | AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens |
| title_short | AHL-Lactonase Producing Psychrobacter sp. From Palk Bay Sediment Mitigates Quorum Sensing-Mediated Virulence Production in Gram Negative Bacterial Pathogens |
| title_sort | ahl lactonase producing psychrobacter sp from palk bay sediment mitigates quorum sensing mediated virulence production in gram negative bacterial pathogens |
| topic | AHL-lactonase marine Palk Bay sediment Pseudomonas aeruginosa Psychrobacter sp. quorum quenching |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.634593/full |
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