Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants
<i>Listeria</i> monocytogenes is a Gram-positive bacterial pathogen and the causative agent of listeriosis, a severe foodborne infection. <i>L. monocytogenes</i> is notorious for its ability to persist in food processing environments (FPEs) via a variety of adaptive traits. E...
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MDPI AG
2021-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/2/199 |
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| author | Phillip Brown Yi Chen Cameron Parsons Eric Brown Martin J. Loessner Yang Shen Sophia Kathariou |
| author_facet | Phillip Brown Yi Chen Cameron Parsons Eric Brown Martin J. Loessner Yang Shen Sophia Kathariou |
| author_sort | Phillip Brown |
| collection | DOAJ |
| description | <i>Listeria</i> monocytogenes is a Gram-positive bacterial pathogen and the causative agent of listeriosis, a severe foodborne infection. <i>L. monocytogenes</i> is notorious for its ability to persist in food processing environments (FPEs) via a variety of adaptive traits. Even though traits such as cold tolerance, biofilm formation and sanitizer resistance have been extensively investigated for their roles in persistence of <i>L. monocytogenes</i> in FPEs, much less is known about resistance to bacteriophages. Previous studies explored phage resistance mechanisms in laboratory-created mutants but it is imperative to investigate phage resistance that is naturally exhibited in FPE-derived strains. Here, we integrated the analysis of whole genome sequence data from a panel of serotype 1/2a strains of sequence types 321 and 391 from turkey processing plants, with the determination of cell surface substituents required for phage adsorption and phage infection assays with the four wide-host-range phages A511, P100, 20422-1 and 805405-1. Using a specific set of recombinant phage protein probes, we discovered that phage-resistant strains lacked one or both of the serogroup 1/2-specific wall teichoic acid carbohydrate decorations, <i>N</i>-acetylglucosamine and rhamnose. Furthermore, these phage-resistant strains harbored substitutions in <i>lmo1080</i>, <i>lmo1081</i>, and <i>lmo2550</i>, which mediate carbohydrate decoration of the wall teichoic acids. |
| first_indexed | 2024-03-09T00:54:33Z |
| format | Article |
| id | doaj.art-74ba8627da0343478b6d72f6b3043625 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:54:33Z |
| publishDate | 2021-02-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-74ba8627da0343478b6d72f6b30436252023-12-11T16:58:29ZengMDPI AGPathogens2076-08172021-02-0110219910.3390/pathogens10020199Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing PlantsPhillip Brown0Yi Chen1Cameron Parsons2Eric Brown3Martin J. Loessner4Yang Shen5Sophia Kathariou6Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USADivision of Microbiology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USADepartment of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USADivision of Microbiology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USALaboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, CH-8092 Zurich, SwitzerlandLaboratory of Food Microbiology, Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, CH-8092 Zurich, SwitzerlandDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA<i>Listeria</i> monocytogenes is a Gram-positive bacterial pathogen and the causative agent of listeriosis, a severe foodborne infection. <i>L. monocytogenes</i> is notorious for its ability to persist in food processing environments (FPEs) via a variety of adaptive traits. Even though traits such as cold tolerance, biofilm formation and sanitizer resistance have been extensively investigated for their roles in persistence of <i>L. monocytogenes</i> in FPEs, much less is known about resistance to bacteriophages. Previous studies explored phage resistance mechanisms in laboratory-created mutants but it is imperative to investigate phage resistance that is naturally exhibited in FPE-derived strains. Here, we integrated the analysis of whole genome sequence data from a panel of serotype 1/2a strains of sequence types 321 and 391 from turkey processing plants, with the determination of cell surface substituents required for phage adsorption and phage infection assays with the four wide-host-range phages A511, P100, 20422-1 and 805405-1. Using a specific set of recombinant phage protein probes, we discovered that phage-resistant strains lacked one or both of the serogroup 1/2-specific wall teichoic acid carbohydrate decorations, <i>N</i>-acetylglucosamine and rhamnose. Furthermore, these phage-resistant strains harbored substitutions in <i>lmo1080</i>, <i>lmo1081</i>, and <i>lmo2550</i>, which mediate carbohydrate decoration of the wall teichoic acids.https://www.mdpi.com/2076-0817/10/2/199<i>Listeria</i>bacteriophagephage resistancewhole genome sequencingfood processing plantInlA |
| spellingShingle | Phillip Brown Yi Chen Cameron Parsons Eric Brown Martin J. Loessner Yang Shen Sophia Kathariou Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants Pathogens <i>Listeria</i> bacteriophage phage resistance whole genome sequencing food processing plant InlA |
| title | Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants |
| title_full | Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants |
| title_fullStr | Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants |
| title_full_unstemmed | Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants |
| title_short | Whole Genome Sequence Analysis of Phage-Resistant <i>Listeria monocytogenes</i> Serotype 1/2a Strains from Turkey Processing Plants |
| title_sort | whole genome sequence analysis of phage resistant i listeria monocytogenes i serotype 1 2a strains from turkey processing plants |
| topic | <i>Listeria</i> bacteriophage phage resistance whole genome sequencing food processing plant InlA |
| url | https://www.mdpi.com/2076-0817/10/2/199 |
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