Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD
Chronic obstructive pulmonary disease (COPD) is caused by exposure to toxic particles, such as coal fly ash (CFA), diesel-exhaust particle (DEP), and cigarette smoke (CS), leading to chronic bronchitis, mucus production, and a subsequent lung dysfunction. This study, using a mouse model of COPD, aim...
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MDPI AG
2020-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/4/926 |
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| author | Seung-Hyung Kim Jung-Hee Hong Won-Kyung Yang Jeong-Ho Geum Hye-Rim Kim Su-Young Choi Yun-Mi Kang Hyo-Jin An Young-Cheol Lee |
| author_facet | Seung-Hyung Kim Jung-Hee Hong Won-Kyung Yang Jeong-Ho Geum Hye-Rim Kim Su-Young Choi Yun-Mi Kang Hyo-Jin An Young-Cheol Lee |
| author_sort | Seung-Hyung Kim |
| collection | DOAJ |
| description | Chronic obstructive pulmonary disease (COPD) is caused by exposure to toxic particles, such as coal fly ash (CFA), diesel-exhaust particle (DEP), and cigarette smoke (CS), leading to chronic bronchitis, mucus production, and a subsequent lung dysfunction. This study, using a mouse model of COPD, aimed to evaluate the effect of herbal combinational medication of <i>Glycyrrhiza glabra</i> (GG), <i>Agastache rugosa</i> (AR) containing glycyrrhizic acid (GA), and tilianin (TN) as active ingredients. GA, a major active component of GG, possesses a range of pharmacological and biological activities including anti-inflammatory, anti-allergic, anti-oxidative. TN is a major flavonoid that is present in AR. It has been reported to have anti-inflammatory effects of potential utility as an anti-COPD agent. The COPD in the mice model was induced by a challenge with CFA and DEP. BALB/c mice received CFA and DEP alternately three times for 2 weeks to induce COPD. The herbal mixture of GG, AR, and TN significantly decreased the number of neutrophils in the lungs and bronchoalveolar lavage (BAL) fluid. It also significantly reduced the production of C-X-C motif chemokine ligand 2 (CXCL-2), IL-17A, CXCL-1, TNF-α, symmetric dimethylarginine (SDMA) in BALF and CXCL-2, IL-17A, CXCL-1, MUC5AC, transient receptor potential vanilloid-1 (TRPV1), IL-6, COX-2, NOS-II, and TNF-α mRNA expression in the lung tissue. Notably, a combination of GG and AR was more effective at regulating such therapeutic targets than GG or AR alone. The histolopathological lung injury was alleviated by treatment with the herbal mixture and their active ingredients (especially TN). In this study, the herbal combinational mixture more effectively inhibited neutrophilic airway inflammation by regulating the expression of inflammatory cytokines and CXCL-2 by blocking the IL-17/STAT3 pathway. Therefore, a herbal mixture of GG and AR may be a potential therapeutic agent to treat COPD. |
| first_indexed | 2024-03-11T10:11:48Z |
| format | Article |
| id | doaj.art-74c104d83eb6489aa418ba9cb6bfc5c1 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T10:11:48Z |
| publishDate | 2020-03-01 |
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| series | Nutrients |
| spelling | doaj.art-74c104d83eb6489aa418ba9cb6bfc5c12023-11-16T14:27:59ZengMDPI AGNutrients2072-66432020-03-0112492610.3390/nu12040926Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPDSeung-Hyung Kim0Jung-Hee Hong1Won-Kyung Yang2Jeong-Ho Geum3Hye-Rim Kim4Su-Young Choi5Yun-Mi Kang6Hyo-Jin An7Young-Cheol Lee8Institute of Traditional Medicine & Bioscience, Daejeon University, Daejeon 34520, KoreaDepartment of Herbology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju, Gangwon-do 26339, KoreaInstitute of Traditional Medicine & Bioscience, Daejeon University, Daejeon 34520, KoreaCOSMAX NBT, INC., Seoul 06132, KoreaCOSMAX NS, INC., Seoul 06132, KoreaCOSMAX NBT, INC., Seoul 06132, KoreaDepartment of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju, Gangwon-do 26339, KoreaDepartment of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju, Gangwon-do 26339, KoreaDepartment of Herbology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju, Gangwon-do 26339, KoreaChronic obstructive pulmonary disease (COPD) is caused by exposure to toxic particles, such as coal fly ash (CFA), diesel-exhaust particle (DEP), and cigarette smoke (CS), leading to chronic bronchitis, mucus production, and a subsequent lung dysfunction. This study, using a mouse model of COPD, aimed to evaluate the effect of herbal combinational medication of <i>Glycyrrhiza glabra</i> (GG), <i>Agastache rugosa</i> (AR) containing glycyrrhizic acid (GA), and tilianin (TN) as active ingredients. GA, a major active component of GG, possesses a range of pharmacological and biological activities including anti-inflammatory, anti-allergic, anti-oxidative. TN is a major flavonoid that is present in AR. It has been reported to have anti-inflammatory effects of potential utility as an anti-COPD agent. The COPD in the mice model was induced by a challenge with CFA and DEP. BALB/c mice received CFA and DEP alternately three times for 2 weeks to induce COPD. The herbal mixture of GG, AR, and TN significantly decreased the number of neutrophils in the lungs and bronchoalveolar lavage (BAL) fluid. It also significantly reduced the production of C-X-C motif chemokine ligand 2 (CXCL-2), IL-17A, CXCL-1, TNF-α, symmetric dimethylarginine (SDMA) in BALF and CXCL-2, IL-17A, CXCL-1, MUC5AC, transient receptor potential vanilloid-1 (TRPV1), IL-6, COX-2, NOS-II, and TNF-α mRNA expression in the lung tissue. Notably, a combination of GG and AR was more effective at regulating such therapeutic targets than GG or AR alone. The histolopathological lung injury was alleviated by treatment with the herbal mixture and their active ingredients (especially TN). In this study, the herbal combinational mixture more effectively inhibited neutrophilic airway inflammation by regulating the expression of inflammatory cytokines and CXCL-2 by blocking the IL-17/STAT3 pathway. Therefore, a herbal mixture of GG and AR may be a potential therapeutic agent to treat COPD.https://www.mdpi.com/2072-6643/12/4/926<i>Glycyrrhiza glabra</i><i>Agastacha rugosa</i>COPDCXCL-2IL-17STAT3 |
| spellingShingle | Seung-Hyung Kim Jung-Hee Hong Won-Kyung Yang Jeong-Ho Geum Hye-Rim Kim Su-Young Choi Yun-Mi Kang Hyo-Jin An Young-Cheol Lee Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD Nutrients <i>Glycyrrhiza glabra</i> <i>Agastacha rugosa</i> COPD CXCL-2 IL-17 STAT3 |
| title | Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD |
| title_full | Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD |
| title_fullStr | Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD |
| title_full_unstemmed | Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD |
| title_short | Herbal Combinational Medication of <i>Glycyrrhiza glabra</i>, <i>Agastache rugosa</i> Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD |
| title_sort | herbal combinational medication of i glycyrrhiza glabra i i agastache rugosa i containing glycyrrhizic acid tilianin inhibits neutrophilic lung inflammation by affecting cxcl2 interleukin 17 stat3 signal pathways in a murine model of copd |
| topic | <i>Glycyrrhiza glabra</i> <i>Agastacha rugosa</i> COPD CXCL-2 IL-17 STAT3 |
| url | https://www.mdpi.com/2072-6643/12/4/926 |
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