Analysis of the RNA Editing Sites and Orthologous Gene Function of Transcriptome and Chloroplast Genomes in the Evolution of Five <i>Deutzia</i> Species
In this study, the chloroplast genomes and transcriptomes of five <i>Deutzia</i> genus species were sequenced, characterized, combined, and analyzed. A phylogenetic tree was constructed, including 32 other chloroplast genome sequences of Hydrangeoideae species. The results showed that th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/16/12954 |
| Summary: | In this study, the chloroplast genomes and transcriptomes of five <i>Deutzia</i> genus species were sequenced, characterized, combined, and analyzed. A phylogenetic tree was constructed, including 32 other chloroplast genome sequences of Hydrangeoideae species. The results showed that the five <i>Deutzia</i> chloroplast genomes were typical circular genomes 156,860–157,025 bp in length, with 37.58–37.6% GC content. Repeat analysis showed that the <i>Deutzia</i> species had 41–45 scattered repeats and 199–201 simple sequence repeats. Comparative genomic and pi analyses indicated that the genomes are conservative and that the gene structures are stable. According to the phylogenetic tree, <i>Deutzia</i> species appear to be closely related to <i>Kirengeshoma palmata</i> and <i>Philadelphus</i>. By combining chloroplast genomic and transcriptomic analyses, 29–31 RNA editing events and 163–194 orthologous genes were identified. The <i>ndh</i>, <i>rpo</i>, <i>rps</i>, and <i>atp</i> genes had the most editing sites, and all RNA editing events were of the C-to-U type. Most of the orthologous genes were annotated to the chloroplast, mitochondria, and nucleus, with functions including energy production and conversion, translation, and protein transport. Genes related to the biosynthesis of monoterpenoids and flavonoids were also identified from the transcriptome of <i>Deutzia</i> spp. Our results will contribute to further studies of the genomic information and potential uses of the <i>Deutzia</i> spp. |
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| ISSN: | 1661-6596 1422-0067 |