Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions
Inorganic arsenic (iAs) contamination in drinking water is a global public health problem, and exposure to iAs is a known risk factor for bladder cancer. Perturbation of urinary microbiome and metabolome induced by iAs exposure may have a more direct effect on the development of bladder cancer. The...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323005146 |
| _version_ | 1797812802458484736 |
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| author | Xushen Chen Ying Cheng Xiaolin Tian Jia Li Xiaodong Ying Qiuyi Zhao Meng Wang Yan Liu Yulan Qiu Xiaoyan Yan Xuefeng Ren |
| author_facet | Xushen Chen Ying Cheng Xiaolin Tian Jia Li Xiaodong Ying Qiuyi Zhao Meng Wang Yan Liu Yulan Qiu Xiaoyan Yan Xuefeng Ren |
| author_sort | Xushen Chen |
| collection | DOAJ |
| description | Inorganic arsenic (iAs) contamination in drinking water is a global public health problem, and exposure to iAs is a known risk factor for bladder cancer. Perturbation of urinary microbiome and metabolome induced by iAs exposure may have a more direct effect on the development of bladder cancer. The aim of this study was to determine the impact of iAs exposure on urinary microbiome and metabolome, and to identify microbiota and metabolic signatures that are associated with iAs-induced bladder lesions. We evaluated and quantified the pathological changes of bladder, and performed 16S rDNA sequencing and mass spectrometry-based metabolomics profiling on urine samples from rats exposed to low (30 mg/L NaAsO2) or high (100 mg/L NaAsO2) iAs from early life (in utero and childhood) to puberty. Our results showed that iAs induced pathological bladder lesions, and more severe effects were noticed in the high-iAs group and male rats. Furthermore, six and seven featured urinary bacteria genera were identified in female and male offspring rats, respectively. Several characteristic urinary metabolites, including Menadione, Pilocarpine, N-Acetylornithine, Prostaglandin B1, Deoxyinosine, Biopterin, and 1-Methyluric acid, were identified significantly higher in the high-iAs groups. In addition, the correlation analysis demonstrated that the differential bacteria genera were highly correlated with the featured urinary metabolites. Collectively, these results suggest that exposure to iAs in early life not only causes bladder lesions, but also perturbs urinary microbiome composition and associated metabolic profiles, which shows a strong correlation. Those differential urinary genera and metabolites may contribute to bladder lesions, suggesting a potential for development of urinary biomarkers for iAs-induced bladder cancer. |
| first_indexed | 2024-03-13T07:43:49Z |
| format | Article |
| id | doaj.art-74c37edf7f8943769366267a3492262e |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T07:43:49Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-74c37edf7f8943769366267a3492262e2023-06-03T04:21:26ZengElsevierEcotoxicology and Environmental Safety0147-65132023-07-01259115010Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesionsXushen Chen0Ying Cheng1Xiaolin Tian2Jia Li3Xiaodong Ying4Qiuyi Zhao5Meng Wang6Yan Liu7Yulan Qiu8Xiaoyan Yan9Xuefeng Ren10School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY, United StatesSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, Shanxi, ChinaSchool of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China; Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY, United States; Corresponding author at: School of Public Health, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.Inorganic arsenic (iAs) contamination in drinking water is a global public health problem, and exposure to iAs is a known risk factor for bladder cancer. Perturbation of urinary microbiome and metabolome induced by iAs exposure may have a more direct effect on the development of bladder cancer. The aim of this study was to determine the impact of iAs exposure on urinary microbiome and metabolome, and to identify microbiota and metabolic signatures that are associated with iAs-induced bladder lesions. We evaluated and quantified the pathological changes of bladder, and performed 16S rDNA sequencing and mass spectrometry-based metabolomics profiling on urine samples from rats exposed to low (30 mg/L NaAsO2) or high (100 mg/L NaAsO2) iAs from early life (in utero and childhood) to puberty. Our results showed that iAs induced pathological bladder lesions, and more severe effects were noticed in the high-iAs group and male rats. Furthermore, six and seven featured urinary bacteria genera were identified in female and male offspring rats, respectively. Several characteristic urinary metabolites, including Menadione, Pilocarpine, N-Acetylornithine, Prostaglandin B1, Deoxyinosine, Biopterin, and 1-Methyluric acid, were identified significantly higher in the high-iAs groups. In addition, the correlation analysis demonstrated that the differential bacteria genera were highly correlated with the featured urinary metabolites. Collectively, these results suggest that exposure to iAs in early life not only causes bladder lesions, but also perturbs urinary microbiome composition and associated metabolic profiles, which shows a strong correlation. Those differential urinary genera and metabolites may contribute to bladder lesions, suggesting a potential for development of urinary biomarkers for iAs-induced bladder cancer.http://www.sciencedirect.com/science/article/pii/S0147651323005146Inorganic arsenicBladder lesionUrinary microbiomeUrinary metabolomeBiomarker |
| spellingShingle | Xushen Chen Ying Cheng Xiaolin Tian Jia Li Xiaodong Ying Qiuyi Zhao Meng Wang Yan Liu Yulan Qiu Xiaoyan Yan Xuefeng Ren Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions Ecotoxicology and Environmental Safety Inorganic arsenic Bladder lesion Urinary microbiome Urinary metabolome Biomarker |
| title | Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions |
| title_full | Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions |
| title_fullStr | Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions |
| title_full_unstemmed | Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions |
| title_short | Urinary microbiota and metabolic signatures associated with inorganic arsenic-induced early bladder lesions |
| title_sort | urinary microbiota and metabolic signatures associated with inorganic arsenic induced early bladder lesions |
| topic | Inorganic arsenic Bladder lesion Urinary microbiome Urinary metabolome Biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323005146 |
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