Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i>
The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus <i>Cichlidogyrus</i> were investigated through a microarray hybridization approach using genomic information from the nematode <i>Caenorhabditis elegans</i>. The effects of two conce...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5889 |
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| author | Citlalic A. Pimentel-Acosta Jorge Ramírez-Salcedo Francisco Neptalí Morales-Serna Emma J. Fajer-Ávila Cristina Chávez-Sánchez Humberto H. Lara Alejandra García-Gasca |
| author_facet | Citlalic A. Pimentel-Acosta Jorge Ramírez-Salcedo Francisco Neptalí Morales-Serna Emma J. Fajer-Ávila Cristina Chávez-Sánchez Humberto H. Lara Alejandra García-Gasca |
| author_sort | Citlalic A. Pimentel-Acosta |
| collection | DOAJ |
| description | The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus <i>Cichlidogyrus</i> were investigated through a microarray hybridization approach using genomic information from the nematode <i>Caenorhabditis elegans</i>. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish. |
| first_indexed | 2024-03-10T17:21:28Z |
| format | Article |
| id | doaj.art-74c61c703eca49ed94608c0f963199a2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:21:28Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-74c61c703eca49ed94608c0f963199a22023-11-20T10:19:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116588910.3390/ijms21165889Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i>Citlalic A. Pimentel-Acosta0Jorge Ramírez-Salcedo1Francisco Neptalí Morales-Serna2Emma J. Fajer-Ávila3Cristina Chávez-Sánchez4Humberto H. Lara5Alejandra García-Gasca6Centro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, MexicoUnidad de Microarreglos, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Mexico City 04510, MexicoCentro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, MexicoCentro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, MexicoCentro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, MexicoDepartment of Biology and South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, TX 78249, USACentro de Investigación en Alimentación y Desarrollo, Unidad Mazatlán en Acuicultura y Manejo Ambiental, Mazatlán, Sinaloa 82112, MexicoThe mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus <i>Cichlidogyrus</i> were investigated through a microarray hybridization approach using genomic information from the nematode <i>Caenorhabditis elegans</i>. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish.https://www.mdpi.com/1422-0067/21/16/5889silver nanoparticlesmonogenean parasitesgene expressionmicroarray analysis<i>Caenorhabditis elegans</i><i>Cichlidogyrus</i> |
| spellingShingle | Citlalic A. Pimentel-Acosta Jorge Ramírez-Salcedo Francisco Neptalí Morales-Serna Emma J. Fajer-Ávila Cristina Chávez-Sánchez Humberto H. Lara Alejandra García-Gasca Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> International Journal of Molecular Sciences silver nanoparticles monogenean parasites gene expression microarray analysis <i>Caenorhabditis elegans</i> <i>Cichlidogyrus</i> |
| title | Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> |
| title_full | Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> |
| title_fullStr | Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> |
| title_short | Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from <i>Caenorhabditis elegans</i> |
| title_sort | molecular effects of silver nanoparticles on monogenean parasites lessons from i caenorhabditis elegans i |
| topic | silver nanoparticles monogenean parasites gene expression microarray analysis <i>Caenorhabditis elegans</i> <i>Cichlidogyrus</i> |
| url | https://www.mdpi.com/1422-0067/21/16/5889 |
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