Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza
Abstract Background Vaccination against seasonal influenza strains is recommended for “high risk” patient groups such as infants, elderly and those with respiratory or circulatory diseases. However, efficacy of the trivalent influenza vaccine (TIV) is poor in many cases and in the event of an influe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-03-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-017-2329-5 |
| _version_ | 1818550674143576064 |
|---|---|
| author | M. D Heath N. J. Swan A. C. Marriott N. J. Silman B. Hallis C. Prevosto K. E. Gooch M. A. Skinner |
| author_facet | M. D Heath N. J. Swan A. C. Marriott N. J. Silman B. Hallis C. Prevosto K. E. Gooch M. A. Skinner |
| author_sort | M. D Heath |
| collection | DOAJ |
| description | Abstract Background Vaccination against seasonal influenza strains is recommended for “high risk” patient groups such as infants, elderly and those with respiratory or circulatory diseases. However, efficacy of the trivalent influenza vaccine (TIV) is poor in many cases and in the event of an influenza pandemic, mono-valent vaccines have been rapidly developed and deployed. One of the main issues with use of vaccine in pandemic situations is the lack of a suitable quantity of vaccine early enough during the pandemic to exert a major influence on the transmission of virus and disease outcome. One approach is to use a dose-sparing regimen which inevitably involves enhancing the efficacy using adjuvants. Methods In this study we compare the use of a novel microcrystalline tyrosine (MCT) adjuvant, which is currently used in a niche area of allergy immunotherapy, for its ability to enhance the efficacy of a seasonal TIV preparation. The efficacy of the MCT adjuvant formulation was compared to alum adjuvanted TIV and to TIV administered without adjuvant using a ferret challenge model to determine vaccine efficacy. Results The MCT was found to possess high protein-binding capacity. In the two groups where TIV was formulated with adjuvant, the immune response was found to be higher (as determined by HAI titre) than vaccine administered without adjuvant and especially so after challenge with a live influenza virus. Vaccinated animals exhibited lower viral loads (as determined using RT-PCR) than control animals where no vaccine was administered. Conclusions The attributes of each adjuvant in stimulating single-dose protection against a poorly immunogenic vaccine was demonstrated. The properties of MCT that lead to the reported effectiveness warrants further exploration in this and other vaccine targets - particularly where appropriate immunogenic, biodegradable and stable alternative adjuvants are sought. |
| first_indexed | 2024-12-12T08:49:38Z |
| format | Article |
| id | doaj.art-74c6227431644a2f83b8a45b1af52176 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T08:49:38Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-74c6227431644a2f83b8a45b1af521762022-12-22T00:30:17ZengBMCBMC Infectious Diseases1471-23342017-03-011711910.1186/s12879-017-2329-5Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenzaM. D Heath0N. J. Swan1A. C. Marriott2N. J. Silman3B. Hallis4C. Prevosto5K. E. Gooch6M. A. Skinner7Allergy Therapeutics Ltd, Dominion WayAllergy Therapeutics Ltd, Dominion WayPublic Health England, PHE PortonPublic Health England, PHE PortonPublic Health England, PHE PortonPublic Health England, PHE PortonPublic Health England, PHE PortonAllergy Therapeutics Ltd, Dominion WayAbstract Background Vaccination against seasonal influenza strains is recommended for “high risk” patient groups such as infants, elderly and those with respiratory or circulatory diseases. However, efficacy of the trivalent influenza vaccine (TIV) is poor in many cases and in the event of an influenza pandemic, mono-valent vaccines have been rapidly developed and deployed. One of the main issues with use of vaccine in pandemic situations is the lack of a suitable quantity of vaccine early enough during the pandemic to exert a major influence on the transmission of virus and disease outcome. One approach is to use a dose-sparing regimen which inevitably involves enhancing the efficacy using adjuvants. Methods In this study we compare the use of a novel microcrystalline tyrosine (MCT) adjuvant, which is currently used in a niche area of allergy immunotherapy, for its ability to enhance the efficacy of a seasonal TIV preparation. The efficacy of the MCT adjuvant formulation was compared to alum adjuvanted TIV and to TIV administered without adjuvant using a ferret challenge model to determine vaccine efficacy. Results The MCT was found to possess high protein-binding capacity. In the two groups where TIV was formulated with adjuvant, the immune response was found to be higher (as determined by HAI titre) than vaccine administered without adjuvant and especially so after challenge with a live influenza virus. Vaccinated animals exhibited lower viral loads (as determined using RT-PCR) than control animals where no vaccine was administered. Conclusions The attributes of each adjuvant in stimulating single-dose protection against a poorly immunogenic vaccine was demonstrated. The properties of MCT that lead to the reported effectiveness warrants further exploration in this and other vaccine targets - particularly where appropriate immunogenic, biodegradable and stable alternative adjuvants are sought.http://link.springer.com/article/10.1186/s12879-017-2329-5VaccineInfluenzaAdjuvantMicrocrystalline tyrosineAluminumFerret |
| spellingShingle | M. D Heath N. J. Swan A. C. Marriott N. J. Silman B. Hallis C. Prevosto K. E. Gooch M. A. Skinner Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza BMC Infectious Diseases Vaccine Influenza Adjuvant Microcrystalline tyrosine Aluminum Ferret |
| title | Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| title_full | Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| title_fullStr | Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| title_full_unstemmed | Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| title_short | Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| title_sort | comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza |
| topic | Vaccine Influenza Adjuvant Microcrystalline tyrosine Aluminum Ferret |
| url | http://link.springer.com/article/10.1186/s12879-017-2329-5 |
| work_keys_str_mv | AT mdheath comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT njswan comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT acmarriott comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT njsilman comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT bhallis comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT cprevosto comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT kegooch comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza AT maskinner comparisonofanovelmicrocrystallinetyrosineadjuvantwithaluminiumhydroxideforenhancingvaccinationagainstseasonalinfluenza |