The sampling strategy of oral microbiome
Abstract There are multiple habitats in the oral cavity with bacteria, fungi, viruses, and protozoa residing in, which together constitute the oral micro‐ecosystem. These microflorae in the oral cavity primarily include saliva, supragingival dental plaque, subgingival dental plaque, submucosal plaqu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
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| Series: | iMeta |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/imt2.23 |
| _version_ | 1811238178295644160 |
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| author | Hongye Lu Peihui Zou Yifei Zhang Qian Zhang Zhibin Chen Feng Chen |
| author_facet | Hongye Lu Peihui Zou Yifei Zhang Qian Zhang Zhibin Chen Feng Chen |
| author_sort | Hongye Lu |
| collection | DOAJ |
| description | Abstract There are multiple habitats in the oral cavity with bacteria, fungi, viruses, and protozoa residing in, which together constitute the oral micro‐ecosystem. These microflorae in the oral cavity primarily include saliva, supragingival dental plaque, subgingival dental plaque, submucosal plaque around implants, plaque in root canals, and plaque on the mucosal surface. The interest and knowledge of the microbiome have dynamically increased with the advancement of technology. Therefore, a reliable, feasible, and practical sampling strategy for the oral microbiome is required for the investigation. This paper introduced the sampling strategy of oral microorganisms, consisting of sample collection, transport, processing, and storage. The materials and devices involved in this study are all commonly used in clinical practice or laboratory. The feasibility and reliability of the sampling methods described in this paper have been verified by multiple studies. |
| first_indexed | 2024-04-12T12:37:21Z |
| format | Article |
| id | doaj.art-74c63b4b41fb47eeb802f04c997c5224 |
| institution | Directory Open Access Journal |
| issn | 2770-596X |
| language | English |
| last_indexed | 2024-04-12T12:37:21Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | iMeta |
| spelling | doaj.art-74c63b4b41fb47eeb802f04c997c52242022-12-22T03:32:52ZengWileyiMeta2770-596X2022-06-0112n/an/a10.1002/imt2.23The sampling strategy of oral microbiomeHongye Lu0Peihui Zou1Yifei Zhang2Qian Zhang3Zhibin Chen4Feng Chen5Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University Hangzhou ChinaDepartment of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology Peking University School and Hospital of Stomatology Beijing ChinaCentral Laboratory, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology Beijing ChinaCentral Laboratory, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology Beijing ChinaDepartment of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology Peking University School and Hospital of Stomatology Beijing ChinaCentral Laboratory, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology Beijing ChinaAbstract There are multiple habitats in the oral cavity with bacteria, fungi, viruses, and protozoa residing in, which together constitute the oral micro‐ecosystem. These microflorae in the oral cavity primarily include saliva, supragingival dental plaque, subgingival dental plaque, submucosal plaque around implants, plaque in root canals, and plaque on the mucosal surface. The interest and knowledge of the microbiome have dynamically increased with the advancement of technology. Therefore, a reliable, feasible, and practical sampling strategy for the oral microbiome is required for the investigation. This paper introduced the sampling strategy of oral microorganisms, consisting of sample collection, transport, processing, and storage. The materials and devices involved in this study are all commonly used in clinical practice or laboratory. The feasibility and reliability of the sampling methods described in this paper have been verified by multiple studies.https://doi.org/10.1002/imt2.23oral cavitymicrobiomesalivaplaquesampling strategy |
| spellingShingle | Hongye Lu Peihui Zou Yifei Zhang Qian Zhang Zhibin Chen Feng Chen The sampling strategy of oral microbiome iMeta oral cavity microbiome saliva plaque sampling strategy |
| title | The sampling strategy of oral microbiome |
| title_full | The sampling strategy of oral microbiome |
| title_fullStr | The sampling strategy of oral microbiome |
| title_full_unstemmed | The sampling strategy of oral microbiome |
| title_short | The sampling strategy of oral microbiome |
| title_sort | sampling strategy of oral microbiome |
| topic | oral cavity microbiome saliva plaque sampling strategy |
| url | https://doi.org/10.1002/imt2.23 |
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