Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i>
DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in <i>Plutella xylostella</i> are still unknown. We identified the full-length cDNAs of <i>PxdsRNase1</i>...
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MDPI AG
2021-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/8/712 |
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| author | Jin-Zhi Chen Ying-Xia Jiang Miao-Wen Li Jian-Wen Li Ben-Hu Zha Guang Yang |
| author_facet | Jin-Zhi Chen Ying-Xia Jiang Miao-Wen Li Jian-Wen Li Ben-Hu Zha Guang Yang |
| author_sort | Jin-Zhi Chen |
| collection | DOAJ |
| description | DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in <i>Plutella xylostella</i> are still unknown. We identified the full-length cDNAs of <i>PxdsRNase1</i>, <i>PxdsRNase2</i>, <i>PxdsRNase3</i>, and <i>PxdsRNase4</i>. Gene expression profile showed that <i>PxdsRNase1</i> was mainly expressed in the hemolymph; and that <i>PxdsRNase2</i> and <i>PxdsRNase3</i> were mainly expressed in the intestinal tract. The expression of <i>PxCht</i> (<i>Chitinase</i> of <i>P. xylostella</i>) in <i>P. xylostella</i> larvae injected with the mixture of dsPxCht (dsRNA of <i>PxCht</i>) and dsPxdsRNase1 (dsRNA of <i>PxdsRNase1</i>), dsPxdsRNase2 (dsRNA of <i>PxdsRNase2</i>), or dsPxdsRNase3 (dsRNA of <i>PxdsRNase3</i>) was significantly higher than that in the larvae injected with the mixture of dsGFP (dsRNA of green fluorescent protein gene, <i>GFP</i>) and dsPxCht; the transcription level of <i>PxCht</i> in the larvae feeding on the mixture of dsPxCht and dsPxdsRNase1, dsPxdsRNase2, or dsPxdsRNase3 was significantly higher than that in the larvae feeding on the mixture of dsPxCht and dsGFP. The recombinant protein of PxdsRNase1 degraded dsRNA rapidly, PxdsRNase3 cleaved dsRNA without complete degradation, and PxdsRNase2 could not degrade dsRNA in vitro. These results suggested that <i>PxdsRNases1</i>, <i>PxdsRNases2</i>, and <i>PxdsRNases3</i> were involved in the dsRNA degradation to reduce RNAi efficiency with different mechanisms. |
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| id | doaj.art-74cf0df4111d475e8803fef5a9d0b9cf |
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| last_indexed | 2024-03-10T08:42:57Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-74cf0df4111d475e8803fef5a9d0b9cf2023-11-22T08:07:26ZengMDPI AGInsects2075-44502021-08-0112871210.3390/insects12080712Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i>Jin-Zhi Chen0Ying-Xia Jiang1Miao-Wen Li2Jian-Wen Li3Ben-Hu Zha4Guang Yang5State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in <i>Plutella xylostella</i> are still unknown. We identified the full-length cDNAs of <i>PxdsRNase1</i>, <i>PxdsRNase2</i>, <i>PxdsRNase3</i>, and <i>PxdsRNase4</i>. Gene expression profile showed that <i>PxdsRNase1</i> was mainly expressed in the hemolymph; and that <i>PxdsRNase2</i> and <i>PxdsRNase3</i> were mainly expressed in the intestinal tract. The expression of <i>PxCht</i> (<i>Chitinase</i> of <i>P. xylostella</i>) in <i>P. xylostella</i> larvae injected with the mixture of dsPxCht (dsRNA of <i>PxCht</i>) and dsPxdsRNase1 (dsRNA of <i>PxdsRNase1</i>), dsPxdsRNase2 (dsRNA of <i>PxdsRNase2</i>), or dsPxdsRNase3 (dsRNA of <i>PxdsRNase3</i>) was significantly higher than that in the larvae injected with the mixture of dsGFP (dsRNA of green fluorescent protein gene, <i>GFP</i>) and dsPxCht; the transcription level of <i>PxCht</i> in the larvae feeding on the mixture of dsPxCht and dsPxdsRNase1, dsPxdsRNase2, or dsPxdsRNase3 was significantly higher than that in the larvae feeding on the mixture of dsPxCht and dsGFP. The recombinant protein of PxdsRNase1 degraded dsRNA rapidly, PxdsRNase3 cleaved dsRNA without complete degradation, and PxdsRNase2 could not degrade dsRNA in vitro. These results suggested that <i>PxdsRNases1</i>, <i>PxdsRNases2</i>, and <i>PxdsRNases3</i> were involved in the dsRNA degradation to reduce RNAi efficiency with different mechanisms.https://www.mdpi.com/2075-4450/12/8/712dsRNaseRNA interferencediamondback mothRNA degradation |
| spellingShingle | Jin-Zhi Chen Ying-Xia Jiang Miao-Wen Li Jian-Wen Li Ben-Hu Zha Guang Yang Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> Insects dsRNase RNA interference diamondback moth RNA degradation |
| title | Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> |
| title_full | Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> |
| title_fullStr | Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> |
| title_full_unstemmed | Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> |
| title_short | Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in <i>Plutella xylostella</i> |
| title_sort | double stranded rna degrading enzymes reduce the efficiency of rna interference in i plutella xylostella i |
| topic | dsRNase RNA interference diamondback moth RNA degradation |
| url | https://www.mdpi.com/2075-4450/12/8/712 |
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