Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley)
In this study, we explored the effect of acid hydrolysis on the molecular, structural, rheological, thermal, and antioxidant characteristics of Qingke β-glucan. The acid hydrolysis reduced the molecular weights of β-glucans from 510 to 155 KDa. The results of the structural analysis by nuclear magne...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1052901/full |
| _version_ | 1797988955954610176 |
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| author | Lan Zhao Shuwei Lin Jingying Lin Jia Wu Huibin Chen |
| author_facet | Lan Zhao Shuwei Lin Jingying Lin Jia Wu Huibin Chen |
| author_sort | Lan Zhao |
| collection | DOAJ |
| description | In this study, we explored the effect of acid hydrolysis on the molecular, structural, rheological, thermal, and antioxidant characteristics of Qingke β-glucan. The acid hydrolysis reduced the molecular weights of β-glucans from 510 to 155 KDa. The results of the structural analysis by nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction, and fourier transforms infrared (FTIR) spectroscopy indicated that acid hydrolysis did not change the primary functional groups of β-glucans. The rheological behavior of β-glucan without and with acid hydrolysis can be described as pseudoplastic and Newtonian, respectively. The DSC curves of the β-glucans with high molecular weights showed the highest transition temperature. The 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity and the reducing power of soluble β-glucans in Qingke showed a dose-dependent pattern. Meanwhile, the antioxidant activities of Qingke β-glucan of different molecular weights were similar. This study demostrated that the acid hydrolysis almost have no effect on antioxidant activity of Qingke β-glucans. |
| first_indexed | 2024-04-11T08:11:36Z |
| format | Article |
| id | doaj.art-74d2404d311e411a9a4e89bd704e8ea6 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T08:11:36Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-74d2404d311e411a9a4e89bd704e8ea62022-12-22T04:35:19ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-11-01910.3389/fnut.2022.10529011052901Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley)Lan Zhao0Shuwei Lin1Jingying Lin2Jia Wu3Huibin Chen4School of Food Science and Engineering, College of Life Sciences, Fujian Normal University, Fuzhou, ChinaSchool of Food Science and Engineering, College of Life Sciences, Fujian Normal University, Fuzhou, ChinaSchool of Food Science and Engineering, College of Life Sciences, Fujian Normal University, Fuzhou, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, ChinaSchool of Food Science and Engineering, College of Life Sciences, Fujian Normal University, Fuzhou, ChinaIn this study, we explored the effect of acid hydrolysis on the molecular, structural, rheological, thermal, and antioxidant characteristics of Qingke β-glucan. The acid hydrolysis reduced the molecular weights of β-glucans from 510 to 155 KDa. The results of the structural analysis by nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction, and fourier transforms infrared (FTIR) spectroscopy indicated that acid hydrolysis did not change the primary functional groups of β-glucans. The rheological behavior of β-glucan without and with acid hydrolysis can be described as pseudoplastic and Newtonian, respectively. The DSC curves of the β-glucans with high molecular weights showed the highest transition temperature. The 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity and the reducing power of soluble β-glucans in Qingke showed a dose-dependent pattern. Meanwhile, the antioxidant activities of Qingke β-glucan of different molecular weights were similar. This study demostrated that the acid hydrolysis almost have no effect on antioxidant activity of Qingke β-glucans.https://www.frontiersin.org/articles/10.3389/fnut.2022.1052901/fullQingke β-glucanphytochemicalsacid hydrolysisstructural characterizationantioxidant activity |
| spellingShingle | Lan Zhao Shuwei Lin Jingying Lin Jia Wu Huibin Chen Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) Frontiers in Nutrition Qingke β-glucan phytochemicals acid hydrolysis structural characterization antioxidant activity |
| title | Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) |
| title_full | Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) |
| title_fullStr | Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) |
| title_full_unstemmed | Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) |
| title_short | Effect of acid hydrolysis on the structural and antioxidant characteristics of β-glucan extracted from Qingke (Tibetan hulless barley) |
| title_sort | effect of acid hydrolysis on the structural and antioxidant characteristics of β glucan extracted from qingke tibetan hulless barley |
| topic | Qingke β-glucan phytochemicals acid hydrolysis structural characterization antioxidant activity |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1052901/full |
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