MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development
Integrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-05-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/50561 |
| _version_ | 1811199672593678336 |
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| author | Kanella Prodromidou Ioannis S Vlachos Maria Gaitanou Georgia Kouroupi Artemis G Hatzigeorgiou Rebecca Matsas |
| author_facet | Kanella Prodromidou Ioannis S Vlachos Maria Gaitanou Georgia Kouroupi Artemis G Hatzigeorgiou Rebecca Matsas |
| author_sort | Kanella Prodromidou |
| collection | DOAJ |
| description | Integrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological relevance of this finding by comparison with data from early to mid-gestation human cortical tissue. Further we find that miR-934 directly controls progenitor to neuroblast transition and impacts on neurite growth of newborn neurons. In agreement, miR-934 targets are involved in progenitor proliferation and neuronal differentiation whilst miR-934 inhibition results in profound global transcriptome changes associated with neurogenesis, axonogenesis, neuronal migration and neurotransmission. Interestingly, miR-934 inhibition affects the expression of genes associated with the subplate zone, a transient compartment most prominent in primates that emerges during early corticogenesis. Our data suggest that mir-934 is a novel regulator of early human neurogenesis with potential implications for a species-specific evolutionary role in brain function. |
| first_indexed | 2024-04-12T01:51:41Z |
| format | Article |
| id | doaj.art-74d31c8439434485ac522ecb044ead93 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:51:41Z |
| publishDate | 2020-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-74d31c8439434485ac522ecb044ead932022-12-22T03:52:55ZengeLife Sciences Publications LtdeLife2050-084X2020-05-01910.7554/eLife.50561MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early developmentKanella Prodromidou0https://orcid.org/0000-0001-5721-9124Ioannis S Vlachos1Maria Gaitanou2Georgia Kouroupi3Artemis G Hatzigeorgiou4Rebecca Matsas5https://orcid.org/0000-0002-4027-348XLaboratory of Cellular and Molecular Neurobiology-Stem Cells, Department of Neurobiology, Hellenic Pasteur Institute, Athens, GreeceDepartment of Pathology, Beth Israel Deaconess Medical Center, Boston, United States; DIANA-Lab, Hellenic Pasteur Institute, Athens, Greece; Harvard Medical School, Boston, United States; Broad Institute of MIT and Harvard, Cambridge, United StatesLaboratory of Cellular and Molecular Neurobiology-Stem Cells, Department of Neurobiology, Hellenic Pasteur Institute, Athens, GreeceLaboratory of Cellular and Molecular Neurobiology-Stem Cells, Department of Neurobiology, Hellenic Pasteur Institute, Athens, GreeceDIANA-Lab, Hellenic Pasteur Institute, Athens, GreeceLaboratory of Cellular and Molecular Neurobiology-Stem Cells, Department of Neurobiology, Hellenic Pasteur Institute, Athens, GreeceIntegrating differential RNA and miRNA expression during neuronal lineage induction of human embryonic stem cells we identified miR-934, a primate-specific miRNA that displays a stage-specific expression pattern during progenitor expansion and early neuron generation. We demonstrate the biological relevance of this finding by comparison with data from early to mid-gestation human cortical tissue. Further we find that miR-934 directly controls progenitor to neuroblast transition and impacts on neurite growth of newborn neurons. In agreement, miR-934 targets are involved in progenitor proliferation and neuronal differentiation whilst miR-934 inhibition results in profound global transcriptome changes associated with neurogenesis, axonogenesis, neuronal migration and neurotransmission. Interestingly, miR-934 inhibition affects the expression of genes associated with the subplate zone, a transient compartment most prominent in primates that emerges during early corticogenesis. Our data suggest that mir-934 is a novel regulator of early human neurogenesis with potential implications for a species-specific evolutionary role in brain function.https://elifesciences.org/articles/50561early neuronsneuritogenesisPAX6doublecortinprogenitor cellneural induction |
| spellingShingle | Kanella Prodromidou Ioannis S Vlachos Maria Gaitanou Georgia Kouroupi Artemis G Hatzigeorgiou Rebecca Matsas MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development eLife early neurons neuritogenesis PAX6 doublecortin progenitor cell neural induction |
| title | MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development |
| title_full | MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development |
| title_fullStr | MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development |
| title_full_unstemmed | MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development |
| title_short | MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development |
| title_sort | microrna 934 is a novel primate specific small non coding rna with neurogenic function during early development |
| topic | early neurons neuritogenesis PAX6 doublecortin progenitor cell neural induction |
| url | https://elifesciences.org/articles/50561 |
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