Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for de...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2003-12-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008 |
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| author | Maria Imaculada Zucchi Rosana Pereira Vianello Brondani José Baldin Pinheiro Lázaro José Chaves Alexandre Siqueira Guedes Coelho Roland Vencovsky |
| author_facet | Maria Imaculada Zucchi Rosana Pereira Vianello Brondani José Baldin Pinheiro Lázaro José Chaves Alexandre Siqueira Guedes Coelho Roland Vencovsky |
| author_sort | Maria Imaculada Zucchi |
| collection | DOAJ |
| description | The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, R ST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure. |
| first_indexed | 2024-12-10T22:17:28Z |
| format | Article |
| id | doaj.art-74d4c79e78a2408ab762fc62c625ac0e |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-10T22:17:28Z |
| publishDate | 2003-12-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-74d4c79e78a2408ab762fc62c625ac0e2022-12-22T01:31:25ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-12-0126444945710.1590/S1415-47572003000400008Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markersMaria Imaculada ZucchiRosana Pereira Vianello BrondaniJosé Baldin PinheiroLázaro José ChavesAlexandre Siqueira Guedes CoelhoRoland VencovskyThe ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, R ST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008SSRMyrtaceaeCerradotropical treegenetic diversity and transferability |
| spellingShingle | Maria Imaculada Zucchi Rosana Pereira Vianello Brondani José Baldin Pinheiro Lázaro José Chaves Alexandre Siqueira Guedes Coelho Roland Vencovsky Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers Genetics and Molecular Biology SSR Myrtaceae Cerrado tropical tree genetic diversity and transferability |
| title | Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers |
| title_full | Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers |
| title_fullStr | Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers |
| title_full_unstemmed | Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers |
| title_short | Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers |
| title_sort | genetic structure and gene flow in eugenia dysenterica dc in the brazilian cerrado utilizing ssr markers |
| topic | SSR Myrtaceae Cerrado tropical tree genetic diversity and transferability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008 |
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