Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview
Prenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of hig...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/12/2239 |
| _version_ | 1797505466696204288 |
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| author | Giulia Sabbatinelli Donatella Fantasia Chiara Palka Elisena Morizio Melissa Alfonsi Giuseppe Calabrese |
| author_facet | Giulia Sabbatinelli Donatella Fantasia Chiara Palka Elisena Morizio Melissa Alfonsi Giuseppe Calabrese |
| author_sort | Giulia Sabbatinelli |
| collection | DOAJ |
| description | Prenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of high-efficiency separation technologies is necessary to obtain the scarce fetal cells from the maternal circulation. Over the years, multiple approaches have been applied, including choice of the ideal cell targets, different cell recovering technologies, and refined cell isolation yield procedures. In order to provide a useful tool and to give insights about limitations and advantages of the technologies available today, we review the genetic research on the creation and validation of non-invasive prenatal diagnostic testing protocols based on the rare and labile circulating fetal cells during pregnancy. |
| first_indexed | 2024-03-10T04:18:59Z |
| format | Article |
| id | doaj.art-74d59ea66bcd46dbb2562a3dbb2687f2 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T04:18:59Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-74d59ea66bcd46dbb2562a3dbb2687f22023-11-23T07:53:20ZengMDPI AGDiagnostics2075-44182021-11-011112223910.3390/diagnostics11122239Isolation and Enrichment of Circulating Fetal Cells for NIPD: An OverviewGiulia Sabbatinelli0Donatella Fantasia1Chiara Palka2Elisena Morizio3Melissa Alfonsi4Giuseppe Calabrese5Dipartimento di Neuroscienze, Imaging & Scienze Cliniche, Scuola Superiore G. D’Annunzio, University of Chieti, 66100 Chieti, ItalyUOSD Genetica Oncoematologica, Dipartimento di Oncologico-Ematologico, Ospedale Spirito Santo, ASL Pescara, 65124 Pescara, ItalyUOC Genetica Medica, Ospedale S.S. Annunziata, ASL2 Chieti, 66100 Chieti, ItalyGenetica Medica, Dipartimento di Tecnologie Avanzate in Medicina e Odontoiatria, School of Medicine, University of Chieti, 66100 Chieti, ItalyUOC Genetica Medica, Ospedale S.S. Annunziata, ASL2 Chieti, 66100 Chieti, ItalyUOSD Genetica Oncoematologica, Dipartimento di Oncologico-Ematologico, Ospedale Spirito Santo, ASL Pescara, 65124 Pescara, ItalyPrenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of high-efficiency separation technologies is necessary to obtain the scarce fetal cells from the maternal circulation. Over the years, multiple approaches have been applied, including choice of the ideal cell targets, different cell recovering technologies, and refined cell isolation yield procedures. In order to provide a useful tool and to give insights about limitations and advantages of the technologies available today, we review the genetic research on the creation and validation of non-invasive prenatal diagnostic testing protocols based on the rare and labile circulating fetal cells during pregnancy.https://www.mdpi.com/2075-4418/11/12/2239prenatal diagnosiscirculating fetal cellsNIPD |
| spellingShingle | Giulia Sabbatinelli Donatella Fantasia Chiara Palka Elisena Morizio Melissa Alfonsi Giuseppe Calabrese Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview Diagnostics prenatal diagnosis circulating fetal cells NIPD |
| title | Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview |
| title_full | Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview |
| title_fullStr | Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview |
| title_full_unstemmed | Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview |
| title_short | Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview |
| title_sort | isolation and enrichment of circulating fetal cells for nipd an overview |
| topic | prenatal diagnosis circulating fetal cells NIPD |
| url | https://www.mdpi.com/2075-4418/11/12/2239 |
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