Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia
(1) Background: The bioavailability of nitric oxide (NO) and oxidative stress are important events related to the pathophysiology of preeclampsia (PE). In this present study, we aimed to evaluate the antioxidant effect of glibenclamide (GB) on the NO synthesis, oxidative stress, and antioxidant capa...
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| author | Priscila Rezeck Nunes Thaina Omia Bueno Pereira Mariana Bertozzi Matheus Nubia Alves Grandini Juliana Silva Siqueira Camila Renata Correa Joelcio Francisco Abbade Valeria Cristina Sandrim |
| author_facet | Priscila Rezeck Nunes Thaina Omia Bueno Pereira Mariana Bertozzi Matheus Nubia Alves Grandini Juliana Silva Siqueira Camila Renata Correa Joelcio Francisco Abbade Valeria Cristina Sandrim |
| author_sort | Priscila Rezeck Nunes |
| collection | DOAJ |
| description | (1) Background: The bioavailability of nitric oxide (NO) and oxidative stress are important events related to the pathophysiology of preeclampsia (PE). In this present study, we aimed to evaluate the antioxidant effect of glibenclamide (GB) on the NO synthesis, oxidative stress, and antioxidant capacity in endothelial cells incubated with plasma from preeclamptic (PE) and normotensive pregnant women (NT). (2) Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with a plasma pool from 10 NT and 10 PE pregnant women; NO/NOx quantification and ROS levels were assessed by a fluorescence compound; lipid peroxidation was evaluated employing thiobarbituric acid (TBA); and total antioxidant capacity was measured by ferric reduction ability power (FRAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). (3) Results: We found that endothelial cells incubated with plasma from PE showed lower NO and NOx levels compared with the NT group. However, GB treatment increased these levels, as well as the antioxidant capacity. Furthermore, a decrease was observed in ROS generation and lipid peroxidation (4) Conclusions: The GB treatment exerted a positive effect on the NO/NOx production by HUVEC incubated with plasma from NT and PE pregnant women, as well as in the reduction in oxidative stress and increase in the antioxidant capacity. |
| first_indexed | 2024-03-09T11:58:33Z |
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| issn | 2076-3921 |
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| last_indexed | 2024-03-09T11:58:33Z |
| publishDate | 2022-08-01 |
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| series | Antioxidants |
| spelling | doaj.art-74d71863b140430da0537e7570db928e2023-11-30T23:06:51ZengMDPI AGAntioxidants2076-39212022-08-01118162010.3390/antiox11081620Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of PreeclampsiaPriscila Rezeck Nunes0Thaina Omia Bueno Pereira1Mariana Bertozzi Matheus2Nubia Alves Grandini3Juliana Silva Siqueira4Camila Renata Correa5Joelcio Francisco Abbade6Valeria Cristina Sandrim7Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (Unesp), Sao Paulo 18618-689, BrazilDepartment of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (Unesp), Sao Paulo 18618-689, BrazilDepartment of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (Unesp), Sao Paulo 18618-689, BrazilDepartment of Pathology, Medical School, Sao Paulo State University (Unesp), Sao Paulo 18618-687, BrazilDepartment of Pathology, Medical School, Sao Paulo State University (Unesp), Sao Paulo 18618-687, BrazilDepartment of Pathology, Medical School, Sao Paulo State University (Unesp), Sao Paulo 18618-687, BrazilDepartment of Gynecology and Obstetrics, Medical School, Sao Paulo State University (Unesp), Sao Paulo 18618-687, BrazilDepartment of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (Unesp), Sao Paulo 18618-689, Brazil(1) Background: The bioavailability of nitric oxide (NO) and oxidative stress are important events related to the pathophysiology of preeclampsia (PE). In this present study, we aimed to evaluate the antioxidant effect of glibenclamide (GB) on the NO synthesis, oxidative stress, and antioxidant capacity in endothelial cells incubated with plasma from preeclamptic (PE) and normotensive pregnant women (NT). (2) Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with a plasma pool from 10 NT and 10 PE pregnant women; NO/NOx quantification and ROS levels were assessed by a fluorescence compound; lipid peroxidation was evaluated employing thiobarbituric acid (TBA); and total antioxidant capacity was measured by ferric reduction ability power (FRAP) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). (3) Results: We found that endothelial cells incubated with plasma from PE showed lower NO and NOx levels compared with the NT group. However, GB treatment increased these levels, as well as the antioxidant capacity. Furthermore, a decrease was observed in ROS generation and lipid peroxidation (4) Conclusions: The GB treatment exerted a positive effect on the NO/NOx production by HUVEC incubated with plasma from NT and PE pregnant women, as well as in the reduction in oxidative stress and increase in the antioxidant capacity.https://www.mdpi.com/2076-3921/11/8/1620glibenclamidepreeclampsianitric oxidereactive oxygen speciesantioxidant capacityHUVEC |
| spellingShingle | Priscila Rezeck Nunes Thaina Omia Bueno Pereira Mariana Bertozzi Matheus Nubia Alves Grandini Juliana Silva Siqueira Camila Renata Correa Joelcio Francisco Abbade Valeria Cristina Sandrim Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia Antioxidants glibenclamide preeclampsia nitric oxide reactive oxygen species antioxidant capacity HUVEC |
| title | Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia |
| title_full | Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia |
| title_fullStr | Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia |
| title_full_unstemmed | Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia |
| title_short | Glibenclamide Increases Nitric Oxide Levels and Decreases Oxidative Stress in an In Vitro Model of Preeclampsia |
| title_sort | glibenclamide increases nitric oxide levels and decreases oxidative stress in an in vitro model of preeclampsia |
| topic | glibenclamide preeclampsia nitric oxide reactive oxygen species antioxidant capacity HUVEC |
| url | https://www.mdpi.com/2076-3921/11/8/1620 |
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