Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements.
After the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. Thus the analysis of the evolution of the optical properties thanks to their...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5510878?pdf=render |
| _version_ | 1828492099090120704 |
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| author | William Moutier Lucile Duforêt-Gaurier Mélilotus Thyssen Hubert Loisel Xavier Mériaux Lucie Courcot David Dessailly Anne-Hélène Rêve Gérald Grégori Séverine Alvain Aude Barani Laurent Brutier Mathilde Dugenne |
| author_facet | William Moutier Lucile Duforêt-Gaurier Mélilotus Thyssen Hubert Loisel Xavier Mériaux Lucie Courcot David Dessailly Anne-Hélène Rêve Gérald Grégori Séverine Alvain Aude Barani Laurent Brutier Mathilde Dugenne |
| author_sort | William Moutier |
| collection | DOAJ |
| description | After the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. Thus the analysis of the evolution of the optical properties thanks to their morphological and/or intra-cellular variations remains poorly studied. Using the Cytosense flow cytometer (CytoBuoy b.v., NL), the temporal course of the forward and sideward efficiencies of two phytoplankton species (Thalassiosira pseudonana and Chlamydomonas concordia) were analyzed during a complete life-cycle. These two species differ considerably from a morphological point of view. Over the whole experiment, the forward and sideward efficiencies of Thalassiosira pseudonana were, on average, respectively 2.2 and 1.6 times higher than the efficiencies of Chlamydomonas concordia. Large intra-species variability of the efficiencies were observed over the life cycle of the considered species. It highlights the importance of considering the optical properties of phytoplankton cells as a function of the population growth stage of the considered species. Furthermore, flow cytometry measurements were combined with radiative transfer simulations and biogeochemical and optical measurements. Results showed that the real refractive index of the chloroplast is a key parameter driving the sideward signal and that a simplistic two-layered model (cytoplasm-chloroplast) seems particularly appropriate to represent the phytoplankton cells. |
| first_indexed | 2024-12-11T11:14:36Z |
| format | Article |
| id | doaj.art-74da4b8dbd3e48d6805f789ba69e3b36 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T11:14:36Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-74da4b8dbd3e48d6805f789ba69e3b362022-12-22T01:09:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018118010.1371/journal.pone.0181180Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements.William MoutierLucile Duforêt-GaurierMélilotus ThyssenHubert LoiselXavier MériauxLucie CourcotDavid DessaillyAnne-Hélène RêveGérald GrégoriSéverine AlvainAude BaraniLaurent BrutierMathilde DugenneAfter the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. Thus the analysis of the evolution of the optical properties thanks to their morphological and/or intra-cellular variations remains poorly studied. Using the Cytosense flow cytometer (CytoBuoy b.v., NL), the temporal course of the forward and sideward efficiencies of two phytoplankton species (Thalassiosira pseudonana and Chlamydomonas concordia) were analyzed during a complete life-cycle. These two species differ considerably from a morphological point of view. Over the whole experiment, the forward and sideward efficiencies of Thalassiosira pseudonana were, on average, respectively 2.2 and 1.6 times higher than the efficiencies of Chlamydomonas concordia. Large intra-species variability of the efficiencies were observed over the life cycle of the considered species. It highlights the importance of considering the optical properties of phytoplankton cells as a function of the population growth stage of the considered species. Furthermore, flow cytometry measurements were combined with radiative transfer simulations and biogeochemical and optical measurements. Results showed that the real refractive index of the chloroplast is a key parameter driving the sideward signal and that a simplistic two-layered model (cytoplasm-chloroplast) seems particularly appropriate to represent the phytoplankton cells.http://europepmc.org/articles/PMC5510878?pdf=render |
| spellingShingle | William Moutier Lucile Duforêt-Gaurier Mélilotus Thyssen Hubert Loisel Xavier Mériaux Lucie Courcot David Dessailly Anne-Hélène Rêve Gérald Grégori Séverine Alvain Aude Barani Laurent Brutier Mathilde Dugenne Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. PLoS ONE |
| title | Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. |
| title_full | Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. |
| title_fullStr | Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. |
| title_full_unstemmed | Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. |
| title_short | Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements. |
| title_sort | evolution of the scattering properties of phytoplankton cells from flow cytometry measurements |
| url | http://europepmc.org/articles/PMC5510878?pdf=render |
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