Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer
Abstract Background: In 1970, human papillomavirus (HPV) was introduced as the main etiologic factor of cervical carcinoma. Since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as PCR have particular importance...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Arak Medical University
2015-06-01
|
| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-3712-en.pdf |
| _version_ | 1818642897840373760 |
|---|---|
| author | Roohollah Dorostkar Mohammad Sadegh Hashemzadeh Mahdi Tat Mohammad Raza Shafaati Mohammad Najar Asl Samaneh Zahiri Yeganeh |
| author_facet | Roohollah Dorostkar Mohammad Sadegh Hashemzadeh Mahdi Tat Mohammad Raza Shafaati Mohammad Najar Asl Samaneh Zahiri Yeganeh |
| author_sort | Roohollah Dorostkar |
| collection | DOAJ |
| description | Abstract
Background: In 1970, human papillomavirus (HPV) was introduced as the main etiologic factor of cervical carcinoma. Since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as PCR have particular importance in accurate, early and definite diagnosis of the virus. So, in this research, our goal is to use a proprietary PCR assay based on L1 gene of human papillomavirus for molecular recognition of HPV and to evaluate its prevalence in patient samples.
Materials and Methods: In this experimental study, after collecting of samples from malignant cervical lesions, the viral DNA was extracted from paraffin blocks of 50 clinical samples and PCR was done by specific primers for L1 gene of human papillomavirus in all samples. After the analysis of PCR products by 2% agarose gel electrophoresis, sensitivity and specificity of the test were also evaluated.
Results: Among 50 patient samples, 33 cases were confirmed to be positive for HPV infection and 17 cases were negative, showing high frequency of HPV in this patient population (about 66%). The results of specificity assay were positive for papilloma samples and sensitivity of the assay was 20 copies of recombinant construct containing L1 per reaction.
Conclusion: This study showed that PCR by specific primers for L1 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between HPV and cervical carcinoma. |
| first_indexed | 2024-12-16T23:50:22Z |
| format | Article |
| id | doaj.art-74da5cd197b74e1092fc388b8a9ca7c2 |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-16T23:50:22Z |
| publishDate | 2015-06-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-74da5cd197b74e1092fc388b8a9ca7c22022-12-21T22:11:22ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2015-06-011832836Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical CancerRoohollah Dorostkar0Mohammad Sadegh Hashemzadeh1Mahdi Tat2Mohammad Raza Shafaati3Mohammad Najar Asl4Samaneh Zahiri Yeganeh5Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Abstract Background: In 1970, human papillomavirus (HPV) was introduced as the main etiologic factor of cervical carcinoma. Since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as PCR have particular importance in accurate, early and definite diagnosis of the virus. So, in this research, our goal is to use a proprietary PCR assay based on L1 gene of human papillomavirus for molecular recognition of HPV and to evaluate its prevalence in patient samples. Materials and Methods: In this experimental study, after collecting of samples from malignant cervical lesions, the viral DNA was extracted from paraffin blocks of 50 clinical samples and PCR was done by specific primers for L1 gene of human papillomavirus in all samples. After the analysis of PCR products by 2% agarose gel electrophoresis, sensitivity and specificity of the test were also evaluated. Results: Among 50 patient samples, 33 cases were confirmed to be positive for HPV infection and 17 cases were negative, showing high frequency of HPV in this patient population (about 66%). The results of specificity assay were positive for papilloma samples and sensitivity of the assay was 20 copies of recombinant construct containing L1 per reaction. Conclusion: This study showed that PCR by specific primers for L1 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between HPV and cervical carcinoma.http://amuj.arakmu.ac.ir/article-1-3712-en.pdfCancer of cervixHuman Papilloma VirusPCR |
| spellingShingle | Roohollah Dorostkar Mohammad Sadegh Hashemzadeh Mahdi Tat Mohammad Raza Shafaati Mohammad Najar Asl Samaneh Zahiri Yeganeh Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Cancer of cervix Human Papilloma Virus PCR |
| title | Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer |
| title_full | Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer |
| title_fullStr | Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer |
| title_full_unstemmed | Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer |
| title_short | Molecular Recognition of Human Papilloma Virus (HPV) Using Proprietary PCR Method Based on L1 Gene and the Evaluation of its Frequency in Tissue Samples from Patients with Cervical Cancer |
| title_sort | molecular recognition of human papilloma virus hpv using proprietary pcr method based on l1 gene and the evaluation of its frequency in tissue samples from patients with cervical cancer |
| topic | Cancer of cervix Human Papilloma Virus PCR |
| url | http://amuj.arakmu.ac.ir/article-1-3712-en.pdf |
| work_keys_str_mv | AT roohollahdorostkar molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer AT mohammadsadeghhashemzadeh molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer AT mahditat molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer AT mohammadrazashafaati molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer AT mohammadnajarasl molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer AT samanehzahiriyeganeh molecularrecognitionofhumanpapillomavirushpvusingproprietarypcrmethodbasedonl1geneandtheevaluationofitsfrequencyintissuesamplesfrompatientswithcervicalcancer |