Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses
Morels (Morchella spp.) are iconic edible mushrooms with a long history of human consumption. Some microbial taxa are hypothesized to be important in triggering the formation of morel primordia and development of fruiting bodies, thus, there is interest in the microbial ecology of these fungi. To id...
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| Format: | Article |
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PeerJ Inc.
2019-09-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/7744.pdf |
| _version_ | 1797423843348840448 |
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| author | Gian Maria Niccolò Benucci Reid Longley Peng Zhang Qi Zhao Gregory Bonito Fuqiang Yu |
| author_facet | Gian Maria Niccolò Benucci Reid Longley Peng Zhang Qi Zhao Gregory Bonito Fuqiang Yu |
| author_sort | Gian Maria Niccolò Benucci |
| collection | DOAJ |
| description | Morels (Morchella spp.) are iconic edible mushrooms with a long history of human consumption. Some microbial taxa are hypothesized to be important in triggering the formation of morel primordia and development of fruiting bodies, thus, there is interest in the microbial ecology of these fungi. To identify and compare fungal and prokaryotic communities in soils where Morchella sextelata is cultivated in outdoor greenhouses, ITS and 16S rDNA high throughput amplicon sequencing and microbiome analyses were performed. Pedobacter, Pseudomonas, Stenotrophomonas, and Flavobacterium were found to comprise the core microbiome of M. sextelata ascocarps. These bacterial taxa were also abundant in the soil beneath growing fruiting bodies. A total of 29 bacterial taxa were found to be statistically associated to Morchella fruiting bodies. Bacterial community network analysis revealed high modularity with some 16S rDNA operational taxonomic unit clusters living in specialized fungal niches (e.g., pileus, stipe). Other fungi dominating the soil mycobiome beneath morels included Morchella, Phialophora, and Mortierella. This research informs understanding of microbial indicators and potential facilitators of Morchella ecology and fruiting body production. |
| first_indexed | 2024-03-09T07:53:37Z |
| format | Article |
| id | doaj.art-74dad3e07ccd4c2a98356619bac40288 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:53:37Z |
| publishDate | 2019-09-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-74dad3e07ccd4c2a98356619bac402882023-12-03T01:20:51ZengPeerJ Inc.PeerJ2167-83592019-09-017e774410.7717/peerj.7744Microbial communities associated with the black morel Morchella sextelata cultivated in greenhousesGian Maria Niccolò Benucci0Reid Longley1Peng Zhang2Qi Zhao3Gregory Bonito4Fuqiang Yu5Plant Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USAMicrobiology and Molecular Genetics, Michigan State University, East Lansing, MI, USACAS Key Laboratory for East Asia Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan, ChinaCAS Key Laboratory for East Asia Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan, ChinaPlant Soil and Microbial Sciences, Michigan State University, East Lansing, MI, USACAS Key Laboratory for East Asia Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan, ChinaMorels (Morchella spp.) are iconic edible mushrooms with a long history of human consumption. Some microbial taxa are hypothesized to be important in triggering the formation of morel primordia and development of fruiting bodies, thus, there is interest in the microbial ecology of these fungi. To identify and compare fungal and prokaryotic communities in soils where Morchella sextelata is cultivated in outdoor greenhouses, ITS and 16S rDNA high throughput amplicon sequencing and microbiome analyses were performed. Pedobacter, Pseudomonas, Stenotrophomonas, and Flavobacterium were found to comprise the core microbiome of M. sextelata ascocarps. These bacterial taxa were also abundant in the soil beneath growing fruiting bodies. A total of 29 bacterial taxa were found to be statistically associated to Morchella fruiting bodies. Bacterial community network analysis revealed high modularity with some 16S rDNA operational taxonomic unit clusters living in specialized fungal niches (e.g., pileus, stipe). Other fungi dominating the soil mycobiome beneath morels included Morchella, Phialophora, and Mortierella. This research informs understanding of microbial indicators and potential facilitators of Morchella ecology and fruiting body production.https://peerj.com/articles/7744.pdfMicrobiomeMicrobial ecologyMorchellaUSEARCHMushroom cultivationCONSTAX |
| spellingShingle | Gian Maria Niccolò Benucci Reid Longley Peng Zhang Qi Zhao Gregory Bonito Fuqiang Yu Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses PeerJ Microbiome Microbial ecology Morchella USEARCH Mushroom cultivation CONSTAX |
| title | Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses |
| title_full | Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses |
| title_fullStr | Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses |
| title_full_unstemmed | Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses |
| title_short | Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses |
| title_sort | microbial communities associated with the black morel morchella sextelata cultivated in greenhouses |
| topic | Microbiome Microbial ecology Morchella USEARCH Mushroom cultivation CONSTAX |
| url | https://peerj.com/articles/7744.pdf |
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