Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran
Background and Objectives: Various infectious and non-infectious factors can cause encephalitis in the central nervous system (CNS), the most important of which are viruses. Herpes viruses are one of the most important causes of encephalitis worldwide. PCR detected the virus on the cerebrospinal fl...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2023-02-01
|
| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3964 |
| _version_ | 1797902798300381184 |
|---|---|
| author | Kamal Fakhredini Hoorieh Soleimanjahi Taravat Bamdad |
| author_facet | Kamal Fakhredini Hoorieh Soleimanjahi Taravat Bamdad |
| author_sort | Kamal Fakhredini |
| collection | DOAJ |
| description |
Background and Objectives: Various infectious and non-infectious factors can cause encephalitis in the central nervous system (CNS), the most important of which are viruses. Herpes viruses are one of the most important causes of encephalitis worldwide. PCR detected the virus on the cerebrospinal fluid (CSF) sample. The aim of this study was to set up an in-house PCR to identify herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) and determine the prevalence of these viruses in suspected children of encephalitis.
Materials and Methods: This cross-sectional study was conducted on 160 suspected children with encephalitis cases referred to Dr. Kermanshahi Children Hospital, Kermanshah, Iran, from April to March 2021. CSF samples were extracted using a viral extraction kit, and a PCR was performed. The level of glucose and total protein of the samples was measured.
Results: The total prevalence of HSV was 16.25%. 17 samples were positive for HSV-1 (10.6%), and 9 samples for HSV-2 (5.6%). There was a significant correlation between glucose, total protein, and HSV PCR positive, but no significant correlation between age and HSV PCR positive results.
Conclusion: Rapid diagnosis of a virus may reduce the hospitalization rate and the use of unnecessary therapies and crease mortality, morbidity, and disability in children. Results in this study show that the distribution of HSV types in children with encephalitis predominantly was type 1 compared with type 2.
|
| first_indexed | 2024-04-10T09:23:19Z |
| format | Article |
| id | doaj.art-74dd02613b2c4b85a5af693ed4fbbd17 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-10T09:23:19Z |
| publishDate | 2023-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-74dd02613b2c4b85a5af693ed4fbbd172023-02-20T08:49:04ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-02-0115110.18502/ijm.v15i1.11931Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, IranKamal Fakhredini0Hoorieh Soleimanjahi1Taravat Bamdad2Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Background and Objectives: Various infectious and non-infectious factors can cause encephalitis in the central nervous system (CNS), the most important of which are viruses. Herpes viruses are one of the most important causes of encephalitis worldwide. PCR detected the virus on the cerebrospinal fluid (CSF) sample. The aim of this study was to set up an in-house PCR to identify herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) and determine the prevalence of these viruses in suspected children of encephalitis. Materials and Methods: This cross-sectional study was conducted on 160 suspected children with encephalitis cases referred to Dr. Kermanshahi Children Hospital, Kermanshah, Iran, from April to March 2021. CSF samples were extracted using a viral extraction kit, and a PCR was performed. The level of glucose and total protein of the samples was measured. Results: The total prevalence of HSV was 16.25%. 17 samples were positive for HSV-1 (10.6%), and 9 samples for HSV-2 (5.6%). There was a significant correlation between glucose, total protein, and HSV PCR positive, but no significant correlation between age and HSV PCR positive results. Conclusion: Rapid diagnosis of a virus may reduce the hospitalization rate and the use of unnecessary therapies and crease mortality, morbidity, and disability in children. Results in this study show that the distribution of HSV types in children with encephalitis predominantly was type 1 compared with type 2. https://ijm.tums.ac.ir/index.php/ijm/article/view/3964Encephalitis;Herpes simplex virus;Cerebrospinal fluid |
| spellingShingle | Kamal Fakhredini Hoorieh Soleimanjahi Taravat Bamdad Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran Iranian Journal of Microbiology Encephalitis; Herpes simplex virus; Cerebrospinal fluid |
| title | Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran |
| title_full | Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran |
| title_fullStr | Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran |
| title_full_unstemmed | Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran |
| title_short | Prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in Kermanshah, Iran |
| title_sort | prevalence of herpes simplex viruses types 1 and 2 infections among suspected children of encephalitis in kermanshah iran |
| topic | Encephalitis; Herpes simplex virus; Cerebrospinal fluid |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3964 |
| work_keys_str_mv | AT kamalfakhredini prevalenceofherpessimplexvirusestypes1and2infectionsamongsuspectedchildrenofencephalitisinkermanshahiran AT hooriehsoleimanjahi prevalenceofherpessimplexvirusestypes1and2infectionsamongsuspectedchildrenofencephalitisinkermanshahiran AT taravatbamdad prevalenceofherpessimplexvirusestypes1and2infectionsamongsuspectedchildrenofencephalitisinkermanshahiran |