A protocol for isolating and imaging large extracellular vesicles or midbody remnants from mammalian cell culture

A protocol for isolating and imaging large extracellular vesicles or midbody remnants from mammalian cell culture

Summary: Traditionally, midbody remnants (MBRs) are isolated from cell culture medium using ultracentrifugation, which is expensive and time consuming. Here, we present a protocol for isolating MBRs or large extracellular vesicles (EVs) from mammalian cell culture using either 1.5% polyethylene glyc...

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Bibliographic Details
Main Authors: Sungjin Park, Smit A. Patel, Elizabeth E. Torr, Ashley-Grace N. Dureke, Alina M. McIntyre, Ahna R. Skop
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Culture
Cell Isolation
Cell Separation/Fractionation
Cancer
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005294

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