Improved selection medium and technique for isolation of Azospirillum irakense

An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogen semi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .Th...

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Bibliographic Details
Main Authors: Khalil M. Khammas, Nada Z. Mehdi, Ali H. Alwan
Format: Article
Language:English
Published: College of Pharmacy / Mustansiriyah University 2012-06-01
Series:Al-Mustansiriyah Journal of Pharmaceutical Sciences
Online Access:https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/235
Description
Summary:An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogen semi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum. The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost
ISSN:1815-0993
2959-183X