Improved selection medium and technique for isolation of Azospirillum irakense

An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogen semi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .Th...

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Main Authors: Khalil M. Khammas, Nada Z. Mehdi, Ali H. Alwan
Format: Article
Language:English
Published: College of Pharmacy / Mustansiriyah University 2012-06-01
Series:Al-Mustansiriyah Journal of Pharmaceutical Sciences
Online Access:https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/235
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author Khalil M. Khammas
Nada Z. Mehdi
Ali H. Alwan
author_facet Khalil M. Khammas
Nada Z. Mehdi
Ali H. Alwan
author_sort Khalil M. Khammas
collection DOAJ
description An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogen semi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum. The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost
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spelling doaj.art-74df30fb66e547b29d8ce8bef22333942024-04-04T06:58:52ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2012-06-0111110.32947/ajps.v11i1.235Improved selection medium and technique for isolation of Azospirillum irakenseKhalil M. Khammas0Nada Z. Mehdi1Ali H. Alwan2Dep. of Biology, College of Science, AL-Mustansyria UniversityDep. of Biology, College of Science, AL-Mustansyria UniversityDep. of Biology, College of Science, AL-Mustansyria University An improved selection medium and technique for Isolation of Azospirillum irakense was developed . This technique is based on the utilization of free-nitrogen semi-solid medium supplement with disaccharide, lactulose, as a sole source of carbon and energy instead of malate with a pH value of 7.4 .This selected medium was found to encourage the growth of A. irakense alone and to prevent the other species of Azospirillum. The improved medium was superior than the other available media .The technique was simply applied to detect A.irakense, showed a high increase in numbers of A.irakense isolates obtaining from the soil rhizosphere and roots, and decreased the time cost https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/235
spellingShingle Khalil M. Khammas
Nada Z. Mehdi
Ali H. Alwan
Improved selection medium and technique for isolation of Azospirillum irakense
Al-Mustansiriyah Journal of Pharmaceutical Sciences
title Improved selection medium and technique for isolation of Azospirillum irakense
title_full Improved selection medium and technique for isolation of Azospirillum irakense
title_fullStr Improved selection medium and technique for isolation of Azospirillum irakense
title_full_unstemmed Improved selection medium and technique for isolation of Azospirillum irakense
title_short Improved selection medium and technique for isolation of Azospirillum irakense
title_sort improved selection medium and technique for isolation of azospirillum irakense
url https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/235
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AT nadazmehdi improvedselectionmediumandtechniqueforisolationofazospirillumirakense
AT alihalwan improvedselectionmediumandtechniqueforisolationofazospirillumirakense