Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i>
Understanding non-target-site resistance (NTSR) to herbicides represents a pressing challenge as NTSR is widespread in many weeds. Using giant duckweed (<i>Spirodela polyrhiza</i>) as a model, we systematically investigated genetic and molecular mechanisms of diquat resistance, which can...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/6/845 |
| _version_ | 1797239583609454592 |
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| author | Martin Höfer Martin Schäfer Yangzi Wang Samuel Wink Shuqing Xu |
| author_facet | Martin Höfer Martin Schäfer Yangzi Wang Samuel Wink Shuqing Xu |
| author_sort | Martin Höfer |
| collection | DOAJ |
| description | Understanding non-target-site resistance (NTSR) to herbicides represents a pressing challenge as NTSR is widespread in many weeds. Using giant duckweed (<i>Spirodela polyrhiza</i>) as a model, we systematically investigated genetic and molecular mechanisms of diquat resistance, which can only be achieved via NTSR. Quantifying the diquat resistance of 138 genotypes, we revealed an 8.5-fold difference in resistance levels between the most resistant and most susceptible genotypes. Further experiments suggested that diquat uptake and antioxidant-related processes jointly contributed to diquat resistance in <i>S. polyrhiza</i>. Using a genome-wide association approach, we identified several candidate genes, including a homolog of dienelactone hydrolase, that are associated with diquat resistance in <i>S. polyrhiza</i>. Together, these results provide new insights into the mechanisms and evolution of NTSR in plants. |
| first_indexed | 2024-04-24T17:53:51Z |
| format | Article |
| id | doaj.art-74e09122552a41bd9126799d93232c89 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T17:53:51Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-74e09122552a41bd9126799d93232c892024-03-27T14:00:50ZengMDPI AGPlants2223-77472024-03-0113684510.3390/plants13060845Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i>Martin Höfer0Martin Schäfer1Yangzi Wang2Samuel Wink3Shuqing Xu4Institute for Organismic and Molecular Evolution (iomE), Johannes Gutenberg University, 55128 Mainz, GermanyInstitute for Organismic and Molecular Evolution (iomE), Johannes Gutenberg University, 55128 Mainz, GermanyInstitute for Organismic and Molecular Evolution (iomE), Johannes Gutenberg University, 55128 Mainz, GermanyInstitute for Evolution and Biodiversity, University of Münster, 48149 Münster, GermanyInstitute for Organismic and Molecular Evolution (iomE), Johannes Gutenberg University, 55128 Mainz, GermanyUnderstanding non-target-site resistance (NTSR) to herbicides represents a pressing challenge as NTSR is widespread in many weeds. Using giant duckweed (<i>Spirodela polyrhiza</i>) as a model, we systematically investigated genetic and molecular mechanisms of diquat resistance, which can only be achieved via NTSR. Quantifying the diquat resistance of 138 genotypes, we revealed an 8.5-fold difference in resistance levels between the most resistant and most susceptible genotypes. Further experiments suggested that diquat uptake and antioxidant-related processes jointly contributed to diquat resistance in <i>S. polyrhiza</i>. Using a genome-wide association approach, we identified several candidate genes, including a homolog of dienelactone hydrolase, that are associated with diquat resistance in <i>S. polyrhiza</i>. Together, these results provide new insights into the mechanisms and evolution of NTSR in plants.https://www.mdpi.com/2223-7747/13/6/845non-targeted-site herbicide resistancediquat<i>Spirodela polyrhiza</i>duckweedGWASdose–response measurements |
| spellingShingle | Martin Höfer Martin Schäfer Yangzi Wang Samuel Wink Shuqing Xu Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> Plants non-targeted-site herbicide resistance diquat <i>Spirodela polyrhiza</i> duckweed GWAS dose–response measurements |
| title | Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> |
| title_full | Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> |
| title_fullStr | Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> |
| title_full_unstemmed | Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> |
| title_short | Genetic Mechanism of Non-Targeted-Site Resistance to Diquat in <i>Spirodela polyrhiza</i> |
| title_sort | genetic mechanism of non targeted site resistance to diquat in i spirodela polyrhiza i |
| topic | non-targeted-site herbicide resistance diquat <i>Spirodela polyrhiza</i> duckweed GWAS dose–response measurements |
| url | https://www.mdpi.com/2223-7747/13/6/845 |
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