New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i>
Yeasts can have additional genetic information in the form of cytoplasmic linear dsDNA molecules called virus-like elements (VLEs). Some of them encode killer toxins. The aim of this work was to investigate the prevalence of such elements in <i>D. hansenii</i> killer yeast deposited in c...
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MDPI AG
2021-09-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/9/615 |
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| author | Xymena Połomska Cécile Neuvéglise Joanna Zyzak Barbara Żarowska Serge Casaregola Zbigniew Lazar |
| author_facet | Xymena Połomska Cécile Neuvéglise Joanna Zyzak Barbara Żarowska Serge Casaregola Zbigniew Lazar |
| author_sort | Xymena Połomska |
| collection | DOAJ |
| description | Yeasts can have additional genetic information in the form of cytoplasmic linear dsDNA molecules called virus-like elements (VLEs). Some of them encode killer toxins. The aim of this work was to investigate the prevalence of such elements in <i>D. hansenii</i> killer yeast deposited in culture collections as well as in strains freshly isolated from blue cheeses. Possible benefits to the host from harboring such VLEs were analyzed. VLEs occurred frequently among fresh <i>D. hansenii</i> isolates (15/60 strains), as opposed to strains obtained from culture collections (0/75 strains). Eight new different systems were identified: four composed of two elements and four of three elements. Full sequences of three new VLE systems obtained by NGS revealed extremely high conservation among the largest molecules in these systems except for one ORF, probably encoding a protein resembling immunity determinant to killer toxins of VLE origin in other yeast species. ORFs that could be potentially involved in killer activity due to similarity to genes encoding proteins with domains of chitin-binding/digesting and deoxyribonuclease NucA/NucB activity, could be distinguished in smaller molecules. However, the discovered VLEs were not involved in the biocontrol of <i>Yarrowia lipolytica</i> and <i>Penicillium roqueforti</i> present in blue cheeses. |
| first_indexed | 2024-03-10T07:09:42Z |
| format | Article |
| id | doaj.art-74e1d7908a8448cea9f28efd08e66c71 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T07:09:42Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-74e1d7908a8448cea9f28efd08e66c712023-11-22T15:30:59ZengMDPI AGToxins2072-66512021-09-0113961510.3390/toxins13090615New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i>Xymena Połomska0Cécile Neuvéglise1Joanna Zyzak2Barbara Żarowska3Serge Casaregola4Zbigniew Lazar5Department of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences (WUELS), 50-375 Wroclaw, PolandSPO, INRAE, Montpellier SupAgro, Université de Montpellier, 34060 Montpellier, FranceDepartment of Microbiology, Laboratory of Microbiome Immunobiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandDepartment of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences (WUELS), 50-375 Wroclaw, PolandINRAE, AgroParisTech, Micalis Institute, CIRM-Levures, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceDepartment of Biotechnology and Food Microbiology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences (WUELS), 50-375 Wroclaw, PolandYeasts can have additional genetic information in the form of cytoplasmic linear dsDNA molecules called virus-like elements (VLEs). Some of them encode killer toxins. The aim of this work was to investigate the prevalence of such elements in <i>D. hansenii</i> killer yeast deposited in culture collections as well as in strains freshly isolated from blue cheeses. Possible benefits to the host from harboring such VLEs were analyzed. VLEs occurred frequently among fresh <i>D. hansenii</i> isolates (15/60 strains), as opposed to strains obtained from culture collections (0/75 strains). Eight new different systems were identified: four composed of two elements and four of three elements. Full sequences of three new VLE systems obtained by NGS revealed extremely high conservation among the largest molecules in these systems except for one ORF, probably encoding a protein resembling immunity determinant to killer toxins of VLE origin in other yeast species. ORFs that could be potentially involved in killer activity due to similarity to genes encoding proteins with domains of chitin-binding/digesting and deoxyribonuclease NucA/NucB activity, could be distinguished in smaller molecules. However, the discovered VLEs were not involved in the biocontrol of <i>Yarrowia lipolytica</i> and <i>Penicillium roqueforti</i> present in blue cheeses.https://www.mdpi.com/2072-6651/13/9/615yeast<i>Debaryomyces hansenii</i>virus-like elements (VLEs)linear dsDNA plasmidskiller activityosmotolerance |
| spellingShingle | Xymena Połomska Cécile Neuvéglise Joanna Zyzak Barbara Żarowska Serge Casaregola Zbigniew Lazar New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> Toxins yeast <i>Debaryomyces hansenii</i> virus-like elements (VLEs) linear dsDNA plasmids killer activity osmotolerance |
| title | New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> |
| title_full | New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> |
| title_fullStr | New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> |
| title_full_unstemmed | New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> |
| title_short | New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast <i>Debaryomyces hansenii</i> |
| title_sort | new cytoplasmic virus like elements vles in the yeast i debaryomyces hansenii i |
| topic | yeast <i>Debaryomyces hansenii</i> virus-like elements (VLEs) linear dsDNA plasmids killer activity osmotolerance |
| url | https://www.mdpi.com/2072-6651/13/9/615 |
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