Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia
Hypoxia, a major inducer of angiogenesis, triggers major changes in gene expression at the transcriptional level. Furthermore, under hypoxia, global protein synthesis is blocked while internal ribosome entry sites (IRES) allow specific mRNAs to be translated. Here, we report the transcriptome and tr...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/50094 |
| _version_ | 1811181461012742144 |
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| author | Fransky Hantelys Anne-Claire Godet Florian David Florence Tatin Edith Renaud-Gabardos Françoise Pujol Leila H Diallo Isabelle Ader Laetitia Ligat Anthony K Henras Yasufumi Sato Angelo Parini Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats |
| author_facet | Fransky Hantelys Anne-Claire Godet Florian David Florence Tatin Edith Renaud-Gabardos Françoise Pujol Leila H Diallo Isabelle Ader Laetitia Ligat Anthony K Henras Yasufumi Sato Angelo Parini Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats |
| author_sort | Fransky Hantelys |
| collection | DOAJ |
| description | Hypoxia, a major inducer of angiogenesis, triggers major changes in gene expression at the transcriptional level. Furthermore, under hypoxia, global protein synthesis is blocked while internal ribosome entry sites (IRES) allow specific mRNAs to be translated. Here, we report the transcriptome and translatome signatures of (lymph)angiogenic genes in hypoxic HL-1 mouse cardiomyocytes: most genes are induced at the translatome level, including all IRES-containing mRNAs. Our data reveal activation of (lymph)angiogenic factor mRNA IRESs in early hypoxia. We identify vasohibin1 (VASH1) as an IRES trans-acting factor (ITAF) that is able to bind RNA and to activate the FGF1 IRES in hypoxia, but which tends to inhibit several IRESs in normoxia. VASH1 depletion has a wide impact on the translatome of (lymph)angiogenesis genes, suggesting that this protein can regulate translation positively or negatively in early hypoxia. Translational control thus appears as a pivotal process triggering new vessel formation in ischemic heart. |
| first_indexed | 2024-04-11T09:18:07Z |
| format | Article |
| id | doaj.art-74e2fd9e102344969fe2b18398f709b8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:18:07Z |
| publishDate | 2019-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-74e2fd9e102344969fe2b18398f709b82022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2019-12-01810.7554/eLife.50094Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxiaFransky Hantelys0Anne-Claire Godet1Florian David2Florence Tatin3Edith Renaud-Gabardos4Françoise Pujol5Leila H Diallo6Isabelle Ader7Laetitia Ligat8Anthony K Henras9Yasufumi Sato10Angelo Parini11Eric Lacazette12Barbara Garmy-Susini13Anne-Catherine Prats14https://orcid.org/0000-0002-5282-3776UMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1031-STROMALAB, Inserm, CNRS ERL5311, Etablissement Français du Sang-Occitanie (EFS), National Veterinary School of Toulouse (ENVT), Université de Toulouse, UPS, Toulouse, FranceUMR 1037-CRCT, Inserm, CNRS, Université de Toulouse, UPS, Pôle Technologique-Plateau Protéomique, Toulouse, FranceUMR 5099-LBME, CBI, CNRS, Université de Toulouse, UPS, Toulouse, FranceDepartment of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, JapanUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceUMR 1048-I2MC, Inserm, Université de Toulouse, UPS, Toulouse, FranceHypoxia, a major inducer of angiogenesis, triggers major changes in gene expression at the transcriptional level. Furthermore, under hypoxia, global protein synthesis is blocked while internal ribosome entry sites (IRES) allow specific mRNAs to be translated. Here, we report the transcriptome and translatome signatures of (lymph)angiogenic genes in hypoxic HL-1 mouse cardiomyocytes: most genes are induced at the translatome level, including all IRES-containing mRNAs. Our data reveal activation of (lymph)angiogenic factor mRNA IRESs in early hypoxia. We identify vasohibin1 (VASH1) as an IRES trans-acting factor (ITAF) that is able to bind RNA and to activate the FGF1 IRES in hypoxia, but which tends to inhibit several IRESs in normoxia. VASH1 depletion has a wide impact on the translatome of (lymph)angiogenesis genes, suggesting that this protein can regulate translation positively or negatively in early hypoxia. Translational control thus appears as a pivotal process triggering new vessel formation in ischemic heart.https://elifesciences.org/articles/50094translational controlhypoxiaangiogenesiscardiomyocyteIRESvasohibin |
| spellingShingle | Fransky Hantelys Anne-Claire Godet Florian David Florence Tatin Edith Renaud-Gabardos Françoise Pujol Leila H Diallo Isabelle Ader Laetitia Ligat Anthony K Henras Yasufumi Sato Angelo Parini Eric Lacazette Barbara Garmy-Susini Anne-Catherine Prats Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia eLife translational control hypoxia angiogenesis cardiomyocyte IRES vasohibin |
| title | Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia |
| title_full | Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia |
| title_fullStr | Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia |
| title_full_unstemmed | Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia |
| title_short | Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia |
| title_sort | vasohibin1 a new mouse cardiomyocyte ires trans acting factor that regulates translation in early hypoxia |
| topic | translational control hypoxia angiogenesis cardiomyocyte IRES vasohibin |
| url | https://elifesciences.org/articles/50094 |
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